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Differential Light Scattering Spectroscopy for Studying Biospecific Assembling of Gold Nanoparticles with Protein or Oligonucleotide Probes

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Abstract

A new method is proposed for studying biospecific interactions in systems of conjugates of colloidal gold nanoparticles. The method is based on measuring the differential spectra of light scattered at 90° within wavelength range 350–800 nm. Addition of complementary component to bioconjugate probe results in the nanoparticle aggregation that can be monitored by the light scattering or extinction spectra. To this end, we developed a special attachment to a Specord M-40 spectrophotometer and a corresponding measurement procedure called by us the differential light scattering spectroscopy. The method is compared with the common spectrophotometry as applied to colloidal gold conjugates to various polymers including proteins and oligonucleotides. Our experiments with the gold nanoparticles of different sizes showed a higher potential sensitivity of the suggested method as compared to spectrophotometry. It is expected that the differential light scattering spectroscopy can be used to develop sensitive analytical biospecific test for various biopolymers.

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Authors and Affiliations

  1. Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410015, Russia

    V. A. Bogatyrev, L. A. Dykman, Ya. M. Krasnov & N. G. Khlebtsov

  2. Saratov State University, Astrakhanskaya ul. 83, Saratov, 410026, Russia

    V. A. Bogatyrev & N. G. Khlebtsov

  3. Luk'yanenko Krasnodar Research Institute of Agriculture, Russian Academy of Agricultural Sciences, Krasnodar, KNIISKh-12, 350012, Russia

    V. K. Plotnikov

Authors
  1. V. A. Bogatyrev
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  2. L. A. Dykman
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  3. Ya. M. Krasnov
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  4. V. K. Plotnikov
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  5. N. G. Khlebtsov
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Bogatyrev, V.A., Dykman, L.A., Krasnov, Y.M. et al. Differential Light Scattering Spectroscopy for Studying Biospecific Assembling of Gold Nanoparticles with Protein or Oligonucleotide Probes. Colloid Journal 64, 671–680 (2002). https://doi.org/10.1023/A:1021585702894

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