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Light- and cytokinin-regulated ftsZ gene expression in excised cucumber cotyledons (Cucumis sativus)

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Abstract

Significant similarities are now known to exist between bacterial celldivision and the division of plastids in higher plants. This is primarily duetothe recently proven requirement of homologues of the bacterial cell divisiongene ftsZ, for plastid division. This report describes thegeneration of three partial FtsZ cDNAs from cucumber byreverse-transcriptase mediated- polymerase chain reaction (RT-PCR) and thesubsequent monitoring of the transcript levels of one ftsZgene by high sensitivity quantitative RT-PCR, in etiolated, excised cucumbercotyledon tissue in response to the exposure to light and the exogenousapplication of the phytohormones, cytokinin and auxin. The results obtainedclearly showed an increase in the transcript levels of theftsZ gene examined in response to exposure to light andtreatment with cytokinin, unlike in the case of auxin treatment, where nosignificant change was observed. These results are further discussed in termsofthe possible involvement of the ftsZ gene in light- andcytokinin- induced plastid division.

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  1. Department of Biochemistry, Indian Institute of Science, Bangalore, 560 012, India

    Rajesh Ullanat & C. Jayabaskaran

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  1. Rajesh Ullanat
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  2. C. Jayabaskaran
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Correspondence to C. Jayabaskaran.

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Ullanat, R., Jayabaskaran, C. Light- and cytokinin-regulated ftsZ gene expression in excised cucumber cotyledons (Cucumis sativus). Plant Growth Regulation 38, 209–218 (2002). https://doi.org/10.1023/A:1021575816558

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