Abstract
Ilex paraguariensis (yerba mate) is a speciesnative to South America of economic importance. Plants of yerba mate were grownfor 35 days in pots under photosynthetically active radiation (PAR) atintensities varying between 1, 4, 20, 50 and 100% of full sunlight in order tostudy growth in relation to gibberellins. Internode length of new shootsincreased under lower PAR. Uniconazole and Prohexadione-Ca (inhibitors ofgibberellin biosynthesis) inhibited shoot length under all light intensities,suggesting that increased levels of gibberellins active on shoot growth mayaccount for longer internodes in shaded plants. Gibberellin A1 andA3 were identified in shoots by capillary gas chromatography-massspectrometry. Increased levels of the GA1 + GA3 pool(measured by gas chromatography-selected ion monitoring with deuterogibberellins as internal standards) were found in shoots of plants grown underdecreasing PAR. However exogenous GA3 at 15, 150 and 1500mM was ineffective in reversing the stunting effect of 100% PAR.Inone experiment GA4 at 150 and 1500 mM lengthened theinternodes of plants grown under full sunlight comparable to those grown in theshade, suggesting a differential effect of “active” gibberellins ofdifferent molecular structure. These and previous results imply that thephotomorphogenetic control of shoot growth by light intensity in Ilexparaguariensis plants grown under different PAR is modulated throughthe levels of endogenous gibberellins.
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References
Clúa A., Bottini R., Brocchi G.N., Bogino J., Luna V. and Montaldi E.R. 1996. Growth habit of Lotus tenuis shoots and the influence of photosynthetic photon flux density, sucrose and endogenous levels of gibberellins A1 and A3. Physiol. Plant. 98: 381–388.
Foster K.R. and Morgan P.W. 1995. Genetic regulation of development in Sorghum bicolor. IX. The ma R 3 allele disrupts diurnal control of gibberellin biosynthesis. Plant Physiol. 108: 337–343.
Fujioka S., Yamane H., Spray C.R., Phinney B.O., Gaskin P., Mac-Millan J. et al. 1990. Gibberellin At3 is biosynthesized from gibberellin A20 via gibberellin A5 in shoots of Zea mays L. Plant Physiol. 94: 127–132.
Hedden P. and Kamiya Y. 1997. Gibberellin biosynthesis: enzymes, genes and their regulation. Ann. Rev. Plant Physiol. Plant Mol. Biol. 48: 431–460.
Ingram T.J., Reid J.B., Murfet I.C., Gaskin P., Willis C.L. and MacMillan J. 1984. Internode length in Pisum. The le gene controls the 3β-hydroxylation of gibberellin A20 to gibberellin A1. Planta 160: 455–463.
Junttila O. 1991. Gibberellins and the Regulation of Shoot Elongation in Woody Plants. In: Takahashi N., Phinney B.O. and MacMillan J. (eds), Gibberellins. Springer-Verlag, New York, pp. 199–210.
Kendrick R.E. and Kronenberg G.H.M. 1994. Photomorphogenesis in Plants. Kluwer Academic Publishers, Dordrecht, The Netherlands, p. 527.
Nakayama M., Yamane H., Murofushi N., Takahashi N., Mander L.N. and Seto H. 1991. Gibberellin biosynthetic pathway and the physiologically active gibberellin in the shoot of Cucumis sativus L. J. Plant Growth Regul. 10: 115–121.
Pharis R.P. 1991. Physiology of Gibberellins in Relation to Floral Initiation and Early Floral Differentiation. In: Takahashi N., Phinney B.O. and MacMillan J. (eds), Gibberellins. Springer-Verlag, New York, pp. 166–178.
Pharis R.P. and King R.W. 1985. Gibberellins and reproductive development in seed plants. Ann. Rev. Plant Physiol. 36: 517– 568.
Phillips A.L. 1998. Gibberellins in Arabidopsis. Plant Physiol. Biochem. 36: 115–124.
Phinney B.O. 1985. Gibberellin A1, dwarfism and shoot elongation in higher plants. Biol. Plant. 27: 172–179.
Piccoli P. and Bottini R. 1996. Gibberellin production in Azospirillum lipoferum cultures is enhanced by light. Biocell 20: 185–196.
Rademacher W. 1991. Inhibitors of gibberellin biosynthesis: applications in agriculture and horticulture. In: Takahashi N., Phinney B.O. and MacMillan J. (eds), Gibberellins. Springer-Verlag, New York, pp. 296–310.
Sansberro P.A. 2000. Estudios fisiológicos acerca del control de la brotación en yerba mate (Ilex paraguariensis St. Hil.). PhD Dissertation, Universidad Nacional de Río Cuarto.
Sansberro P.A., Mroginski L.A. and Bottini R. 2001. In vitro morphogenetic responses of Ilex paraguariensis nodal segments treated with different gibberellins and Prohexadione-Ca. Plant Growth Regul. 34: 209–214.
Smith V., Albone K. and MacMillan J. 1991. Enzymatic 3β-hydroxylation of gibberellin A20 and A5. In: Takahashi N., Phinney B.O. and MacMillan J. (eds), Gibberellins. Springer-Verlag, New York, pp. 62–71.
Spray C.R., Phinney B.O., Gaskin P., Gilmour S.J. and MacMillan J. 1984. Internode length in Zea mays L. The dwarf — 1 mutation controls the 3β-hydroxylation gibberellin A20 to gibberellin A1. Planta 160: 464–468.
Volmaro C., Pontin M., Luna V., Baraldi R. and Bottini R. 1998. Blue light control of hypocotyl elongation in etiolated seedlings of Lactuca sativa (L.) cv. Grand Rapids related to exogenous growth regulators and endogenous IAA, GA3 and abscisic acid. Plant Growth Regul. 26: 165–173.
Xu Y.-L., Li L., Gage D.A. and Zeevaart J.A.D. 1999. Feedback regulation of GA5 expression and metabolic engineering of gibberellin levels in Arabidopsis. Plant Cell 11: 927–935.
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Sansberro, P.A., Mroginski, L.A., Masciarelli, O.A. et al. Shoot growth in Ilex paraguariensis plants grown under varying photosynthetically active radiation is affected through gibberellin levels. Plant Growth Regulation 38, 231–236 (2002). https://doi.org/10.1023/A:1021567614741
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DOI: https://doi.org/10.1023/A:1021567614741