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A fiber-FISH contig spanning the non-recombining region of the human Y chromosome

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Abstract

Using fluorescence in-situ hybridization on interphase chromatin fibers (fiber-FISH), we have constructed an overlapping fiber-FISH contig spanning the non-recombining region of the human Y chromosome (NRY). We first established a standard FISH-signal pattern for a distinct panel of DNA clones on prometaphase Y chromosomes in six healthy fertile men. Clones in the panel were selected from all R-bands as well as deletion intervals 1 through 7 plus PAR1 and PAR2 of the human Y chromosome. We next used signals of these marker clones to build a fiber-FISH contig for the multicopy gene families, CDY, DAZ, RBMY, TSPY and XKRY, along the NRY. Our fiber-FISH contig of human NRY may help to close the four gaps that still exist in the current physical map of the human Y chromosome. Furthermore, it provides a more complete picture with respect to the positions and arrangements of the multicopy gene families along the human NRY.

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Authors and Affiliations

  1. Institute of Human Genetics and Anthropology, University of Freiburg, Breisacher Str. 33, 79106, Freiburg, Germany

    Susanne Röttger & Werner Schempp

  2. Division of Medical Genetics, Harbor-UCLA Medical Center, 1000 West Carson Street, Torrance, CA, 90502-2064, USA

    Pauline H. Yen

Authors
  1. Susanne Röttger
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  2. Pauline H. Yen
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  3. Werner Schempp
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Röttger, S., Yen, P.H. & Schempp, W. A fiber-FISH contig spanning the non-recombining region of the human Y chromosome. Chromosome Res 10, 621–635 (2002). https://doi.org/10.1023/A:1021556108571

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  • DOI: https://doi.org/10.1023/A:1021556108571

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