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Characterisation of the ACC oxidase activity in transgenic auxin overproducing tomato during ripening

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Abstract

As part of our studies on the role of auxin in regulating the ethylenebiosynthesis during fruit ripening, in this paper we describe the functionalproperties of the ACC oxidase activity extracted from transgenic tomato(Lycopersicum esculentum Mill. cv. Ailsa craig)overexpressing the tryptophan monooxygenase or iaaM protein fromAgrobacterium tumefaciens that increases the auxin levels.Maximal activity was recovered by extracting the enzyme at pH 8.0 from fruitspicked three days after the onset of the colour change. The enzyme exhibits ahalf-life of 85 min, two relative maxima at 30 and 38°C, an optimum pH of 7.9 and an apparent Km forACC of 118 μM. Our results also show the first evidence of anallosteric type kinetic of the ACC oxidase activity with respect to itscosubstrate ascorbate, with an apparent Km of 12.5mM,estimated as the concentration which gave 50% Vmax.

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Authors and Affiliations

  1. Dept. Fisiología y Tecnología de Productos Vegetales, Instituto de la Grasa. CSIC, E-41012, Seville, Spain

    José Maria Castellano & Blanca Vioque

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  1. José Maria Castellano
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  2. Blanca Vioque
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Correspondence to Blanca Vioque.

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Castellano, J.M., Vioque, B. Characterisation of the ACC oxidase activity in transgenic auxin overproducing tomato during ripening. Plant Growth Regulation 38, 203–208 (2002). https://doi.org/10.1023/A:1021519715650

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