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RFLP/AFLP mapping of a brown planthopper (Nilaparvata lugens Stål) resistance gene Bph1 in rice

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Abstract

We have constructed a linkage map of the rice brown planthopper (BPH)resistance gene, Bph1. RFLP and AFLP markers were selected by thebulked segregant analysis and used in the mapping study of 262 F2sthat were derived from a cross of `Tsukushibare', a susceptible japonica cultivar, and `Norin-PL3', an authentic japonicaBph1-introgression line. Twenty markers were mapped within a 28.9-cMregion containing the Bph1 locus on the long arm of rice chromosome12. Combining the result of segregation analysis of BPH resistance by themass seedling test and that of the markers, the Bph1 locus wasmapped within a 5.8-cM region between two flanking markers. The closestAFLP markers, em5814N and em2802N, was at 2.7 cM proximal to theBph1 locus. Together with the previously constructed high-resolutionmap of bph2 locating the locus at ca. 10 cM proximal to the Bph1 locus, this improved version of the linkage map would facilitatepyramiding these two important BPH resistance genes.

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References

  • Alam, S.N. & M.B. Cohen, 1998. Detection and analysis of QTLs for resistance to the brown planthopper, Nilaparvata lugens, in a doubled-haploid rice population. Theor Appl Genet 97: 1370-1379.

    Google Scholar 

  • Athwal, D.S., M.D. Pathak, E. Bacalangco & C.D. Pura, 1971. Genetics of resistance to brown planthoppers and green leaf hoppers in Oryza sativa L. Crop Sci 11: 747-750.

    Google Scholar 

  • Harushima, Y., M. Yano, A. Shomura, M. Sato, T. Shimano, Y. Kuboki, T. Yamamoto, S.Y. Lin, B.A. Antonio, A. Parco, H. Kajiya, N. Huang, K. Tamamoto, Y. Nagamura, N. Kurata, G. S. Khush & T. Sakaki, 1998. A high-density rice genetic map with 2275 markers using a single F2 population. Genetics 148: 479-494.

    Google Scholar 

  • Hirabayashi, H. & T. Ogawas, 1995. RFLP mapping of Bph-1 (brown planthopper resistance gene) in rice. Jpn J Breed 45: 369-371.

    Google Scholar 

  • Hirabayashi, H., E.R. Angeles, R. Kaji, T. Ogawa, D.S. Brar & G.S. Khush, 1998. Identification of brown planthopper resistance gene derived from O. officinalis using molecular markers in rice. Breed Sci 48 (Suppl. 1), p. 82 (abstract in Japanese).

    Google Scholar 

  • Hirabayashi, H., R. Kaji, E.R. Angeles, T. Ogawa, D.S. Brar & G.S. Khush, 1999. RFLP analysis of a new gene for resistance to brown planthopper derived from O. officinalis on rice chromosome 4. Breed Sci 49 (Suppl. 1), p. 48 (abstract in Japanese).

    Google Scholar 

  • International Rice Research Institute (IRRI), 1976. Selection and studies on sources of resistance: Biotypes. IRRI Ann Rep 1975: 107-108.

    Google Scholar 

  • Ishii, T., D.S. Brar, D.S. Multani & G.S. Khush, 1994. Molecular tagging of genes for brown planthopper resistance and earliness introgressed from Oryza australiensis into cultivated rice, O. sativa. Genome 37: 217-221.

    Google Scholar 

  • Kaneda, C., H. Nemoto, R. Ikeda, M. Yokoo & A. Kobayashi, 1985. Breeding of rice Norin-PL3, a new germplasm with brown planthopper resistance. Bull Natl Agric Res Cent 5: 93-103. (in Japanese)

    Google Scholar 

  • Kawaguchi, M., K. Murata, T. Ishii, S. Takumi, N. Mori & C. Nakamura, 2001. Assignment of a brown planthopper (Nilaparvata lugens Stål) resistance gene bph4 to the rice chromosome 6. Breed Sci 51: 13-18.

    Google Scholar 

  • Ketipearachchi, Y., C. Kaneda & C. Nakamura, 1998. Adaptation of the brown planthopper (BPH), Nilaparvata lugens Stål (Homoptera: Delphacidae), to BPH resistant rice cultivars carrying bph8 or Bph9. Appl Entomol Zool 33: 497-505.

    Google Scholar 

  • Kosambi, D.D., 1944. The estimation of map distance from recombination values. Ann Eegen 12: 172-175.

    Google Scholar 

  • Lander, E.S., P. Green, J. Abrahamson, A. Barlow, M.J. Daly, S.E. Lincoln & L. Newburg, 1987. MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1: 174-181.

    Google Scholar 

  • Mano, Y., S. Kawasaki, F. Takaiwa & T. Komatsuda, 2001. Construction of a genetic map of barley (Hordeum vulgare L.) cross0020'Azumamugi' x 'Kanto Nakate Gold' using a simple and efficient amplified fragment-length polymorphism system. Genome 44: 284-292.

    Google Scholar 

  • Michelmore, R.W., I. Paran & R.V. Kesseli, 1991. Identification of markers liked to disease resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88: 9828-9832.

    Google Scholar 

  • Murai, H., Z. Hashimoto, P.N. Sharma, T. Shimizu, K. Murata, S. Takumi, N. Mori, S. Kawasaki & C. Nakamura, 2001. Construction of a high-resolution linkage map of a rice brown planthopper (Nilaparvata lugens Stål) resistance gene bph2. Theor Appl Genet 103: 526-532.

    Google Scholar 

  • Murata, K., C. Nakamura, M. Fujiwara, N. Mori N. & C. Kaneda, 1997. Tagging and mapping of brown planthopper resistance genes in rice. In: J.-C. Su (Ed.), Proc. 5th Int. Symp. on Rice Molecular Biology, pp. 217-231. Yi-Hsien Pub, Taipei, Taiwan.

  • Murata, K., M. Fujiwara, C. Kaneda, S. Takumi, N. Mori & C. Nakamura, 1998. RFLP mapping of a brown planthopper (Nilaparvata lugens Stål) resistance gene bph2 of indica rice introgressed into a japonica breeding line 'Norin-PL4'. Genes Genet Syst 73: 359-364.

    Google Scholar 

  • Murata, K., M. Fujiwara, H. Murai, S. Takumi, N. Mori & C. Nakamura, 2001. Mapping of a brown planthopper (Nilaparvata lugens Stål) resistance gene Bph9 on the long arm of rice chromosome 12. Cereal Res Commun 29: 245-250.

    Google Scholar 

  • Panda, N. & G.S. Khush, 1995. Factors affecting expression of resistance. In: N. Panda & G.S. Khush (Eds.), Host Plant Resistance to Insects, pp. 207-228. CAB International, Wallingford, U.K.

    Google Scholar 

  • Pathak, M.D. & E.A. Heinrichs, 1982. Selection of biotype populations 2 and 3 of Nilaparvata lugens by exposure to resistant rice varieties. Environ Entomol 11: 85-90.

    Google Scholar 

  • Pathak, M.D. & G.S. Khush, 1979. Studies of varietal resistance in rice to the brown planthopper at IRRI. In: The Brown Planthopper: Threat to Rice Production in Asia, pp. 287-301. IRRI, Ros banos, Phillipines.

    Google Scholar 

  • Sogawa, K., 1982. The rice brown planthopper: Feeding physiology and host plant interactions. Ann Rev Entomol 27: 49-73.

    Google Scholar 

  • Takahashi, A., K. Ito, J. Tang, G. Hu & T. Wada, 1994. Biotypical property in the populations of the brown planthopper, Nilaparvata lugens Stål (Homoptera: Delphacidae), collected in China and Japan. Appl Entomol Zool 29: 461-463.

    Google Scholar 

  • Vos, P., R. Hogers, M. Bleeker, M. Reijans, T.V.D. Lee, M. Hornes, A. Frijters, J. Pot, J. Peleman, M. Kuiper & M. Zabeau, 1995. AFLP: A new technique for DNA fingerprinting. Nucleic Acid Res 23: 4407-4414.

    Google Scholar 

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Authors and Affiliations

  1. Laboratory of Plant Genetics, Department of Biological and Environmental Science, Faculty of Agriculture, and Graduate School of Science and Technology, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, 657-8501, Japan

    P.N. Sharma, A. Torii, S. Takumi, N. Mori & C. Nakamura

  2. Regional Agricultural Research and Development Center, Aralaqanwila, Sri Lanka

    Y. Ketipearachchi

  3. Toyama Prefectural Agriculture Research Center, Yoshioka 1124-1, Toyama, Japan, 939-8153

    K. Murata

Authors
  1. P.N. Sharma
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  2. Y. Ketipearachchi
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  3. K. Murata
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  4. A. Torii
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  5. S. Takumi
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  6. N. Mori
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  7. C. Nakamura
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Sharma, P., Ketipearachchi, Y., Murata, K. et al. RFLP/AFLP mapping of a brown planthopper (Nilaparvata lugens Stål) resistance gene Bph1 in rice. Euphytica 129, 109–117 (2003). https://doi.org/10.1023/A:1021514829783

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