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Identification of a sequence-tagged site (STS) marker linked to a restorer gene for Ogura cytoplasmic male sterility in radish (Raphanus sativus L.) by non-radioactive AFLP analysis

Euphytica volume 129, pages 61–68 (2003)Cite this article

Abstract

To identify DNA markers linked to a fertility restorer (Rf) genefor Ogura cytoplasmic male sterility in radish (Raphanus sativus L.),a non-radioactive, amplified fragment length polymorphism (AFLP) analysiswas performed on bulked DNA samples from male-sterile and male-fertileradishes. Ten male-fertile and 10 male-sterile plants selected arbitrarilyfrom an F2 population made by selfing of F1 plant from a crossbetween a male-sterile (`MS-Gensuke') plant and a restorer (`Comet') plantwere used as material. Using 32 AFLP primer pairs, one AFLP fragment(AFLP190) which is specific to the bulked DNA samples from male-fertileF2 plants was identified. AFLP190 was characterized by molecularcloning and nucleotide sequencing, and was converted to a sequence-taggedsite (STS) marker, STS190. A linkage analysis performed in 126individuals of two independent F2 populations showed tight linkageof STS190 to the Rf gene. The rate of recombination between themarker and Rf was estimated to be less than 1%, making STS1901.2 cM from the gene.

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References

  • Ashikawa, I., Y. Fukuta, K. Tamura & T. Yagi, 2000. Application of AFLP technique that use non-radioactive fluorescent primers to the detection of genetic diversity in Japanese rice cultivars and cloning of DNA sequences derived from an Indica genome. Breed Sci 49: 225-231.

    Google Scholar 

  • Bonhomme, S., F. Budar, D. Lancelin, I. Small, M.C. Defrance & G. Pelletier, 1992. Sequence and transcript analysis of the Nco2.5 Ogura-specific fragment correlated with cytoplasmic male sterility in Brassica cybrids. Mol Gen Genet 235: 340-348.

    Google Scholar 

  • Brennicke, A. & U. Kück, 1993. Plant Mitochondria with Emphasis on RNA Editing and Cytoplasmic Male-Sterility, VCH, Weinheim.

    Google Scholar 

  • Chalhoub, B.A., S. Thibault, V. Laucou, C. Rameau, H. Hofte & R. Cousin, 1997. Silver staining and recovery of AFLP amplification products on large denaturing polyacrylamide gels. Biotechniques 22: 216-220.

    Google Scholar 

  • Dellaporta, S.J., J. Wood & J.B. Hicks, 1983. A plant DNA minipreparation: Version II. Plant Mol Biol Rep 1: 19-21.

    Google Scholar 

  • Delourme, R., A. Bouchereau, N. Hubert, M. Renard & B.S. Landry, 1994. Identification of RAPD markers linked to a fertility restorer gene for the Ogura radish cytoplasmic male sterility of rapeseed (Brassica napus L.). Theor Appl Genet 88: 741-748.

    Google Scholar 

  • Delourme, R. & F. Eber, 1992. Linkage between an isozyme marker and a restorer gene in radish cytoplasmic male sterility of rapeseed (Brassica napus L.). Theor Appl Genet 85: 222-228.

    Google Scholar 

  • Delourme, R., N. Foiset, R. Horvais, P. Barret, G. Champagne, W.Y. Cheung, B.S. Landry & M. Renard, 1998. Characterization of the radish introgression carrying the Rfo restorer gene for the Ogu-INRA cytoplasmic male sterility in rapeseed (Brassica napus L.). Theor Appl Genet 97: 129-134.

    Google Scholar 

  • Grelon, M., F. Budar, S. Bonhomme & G. Pelletier, 1994. Ogura cytoplasmic male-sterility (CMS)-associated orf138 is translated into a mitochondrial membrane polypeptide in male-sterile Brassica cybrids. Mol Gen Genet 243: 540-547.

    Google Scholar 

  • Iwabuchi, M., N. Koizuka, H. Fujimoto, T. Sakai & J. Imamura, 1999. Identification and expression of the kosena radish (Raphanus sativus cv. Kosena) homologue of the ogura radish CMS-associated gene, orf138. Plant Mol Biol 39: 183-188.

    Google Scholar 

  • Krishnasamy, S. & C.A. Makaroff, 1994. Organ-specific reduction in the abundance of a mitochondrial protein accompanies fertility restoration in cytoplasmic male-sterile radish. Plant Mol Biol 26: 935-946.

    Google Scholar 

  • Lander, E.S., P. Green, J. Abrahamson, A. Barlow, M.J. Daly, S.E. Lincoln & L. Newburg, 1987. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1: 174-181.

    Google Scholar 

  • Makaroff, C.A. & J.D. Palmer, 1988. Mitochondrial DNA rearrangements and transcriptional alterations in the male-sterile cytoplasm of Ogura radish. Mol Cell Biol 8: 1474-1480.

    Google Scholar 

  • Michelmore, R.W., I. Paran & R.V. Kesseli, 1991. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88: 9828-9832.

    Google Scholar 

  • Murayama, S., H. Yamagishi & T. Terachi, 1999. Identification of RAPD and SCAR markers linked to a restorer gene for Ogura cytoplasmic male sterility in radish (Raphanus sativus L.) by bulked segregant analysis. Breed Sci 49: 115-121.

    Google Scholar 

  • Ogura, H., 1968. Studies on the new male-sterility in Japanese radish, with special references to the utilization of this sterility towards the practical raising of hybrid seeds. Mem Fac Agric Kagoshima Univ 6: 39-78. Sambrook, J., E.F. Fritsch & T. Maniatis, 1989. Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York.

    Google Scholar 

  • Vos, P., R. Hogers, M. Bleeker, M. Reijans, T. van de Lee, M. Hornes, A. Frijters, J. Pot, J. Peleman, M. Kuiper et al., 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23: 4407-4414.

    Google Scholar 

  • Vrieling, K., J. Peters & H. Sandbrink, 1997. Amplified fragment length polymorphisms (AFLPs) detected with non-radioactive digoxigenine labelled primers in three plant species. Plant Mol Biol Rep 15: 255-262.

    Google Scholar 

  • Wu, Y., L. Tulsieram, Q. Tao, H.B. Zhang & S.J. Rothstein, 2000. A binary vector-based large insert library for Brassica napus and identification of clones linked to a fertility restorer locus for Ogura cytoplasmic male sterility (CMS). Genome 43: 102-109.

    Google Scholar 

  • Wu, Y., L. Tulsieram & S.J. Rothstein, 2001. Identification and characterization of a putative light-harvesting chlorophyll a/bbinding protein gene encoded at a fertility restorer locus for the Ogura CMS in Brassica napus L. Theor Appl Genet 102: 759-766.

    Google Scholar 

  • Xu, M., E. Huaracha & S.S. Korban, 2001. Development of sequence-characterized amplified regions (SCARs) from amplified fragment length polymorphism (AFLP) markers tightly linked to the Vf gene in apple. Genome 44: 63-70.

    Google Scholar 

  • Yamagishi, H., 1998. Distribution and allelism of restorer genes for Ogura cytoplasmic male sterility in wild and cultivated radishes. Genes Genet Syst 73: 79-83.

    Google Scholar 

  • Yamagishi, H. & T. Terachi, 1994a. Molecular and biological studies on male sterile cytoplasm in Cruciferae. I. The origin and distribution of Ogura male sterile cytoplasm in Japanese wild radishes (Raphanus sativus L.) revealed by PCR-aided assay of their mitochondrial DNAs. Theor Appl Genet 87: 996-1000.

    Google Scholar 

  • Yamagishi, H. & T. Terachi, 1994b. Molecular and biological studies on male sterile cytoplasm in Cruciferae. II. The origin of Ogura male sterile cytoplasm inferred from the segregation pattern of male sterility in the F1 progeny of wild and cultivated radishes (Raphanus sativus L.). Euphytica 80: 201-206.

    Google Scholar 

  • Yamagishi, H. & T. Terachi, 1997. Molecular and biological studies on male sterile cytoplasm in Cruciferae. IV. Ogura-type cytoplasm found in the wild radish, Raphanus raphanistrum. Plant Breed 116: 323-329.

    Google Scholar 

  • Yamagishi, H., T. Terachi, Y. Fujita & H. Fukuya, 1993. Induction of male sterility in hybrids between Japanese wild and cultivated radishes (Raphanus sativus L.). Jpn J Breed 43: 635-639.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Engineering, Kyoto Sangyo University, Motoyama, Kamigamo, Kita-ku, Kyoto, 603-8555, Japan

    Seiji Murayama, Toshiyuki Habuchi, Hiroshi Yamagishi & Toru Terachi

  2. Laboratory of Plant Genecology, Hokkaido National Agricultural Experiment Station, Hitsuji-ga-oka, Toyohira-ku, Sapporo, 062-8555, Japan

    Seiji Murayama

Authors
  1. Seiji Murayama
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  2. Toshiyuki Habuchi
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  3. Hiroshi Yamagishi
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  4. Toru Terachi
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Murayama, S., Habuchi, T., Yamagishi, H. et al. Identification of a sequence-tagged site (STS) marker linked to a restorer gene for Ogura cytoplasmic male sterility in radish (Raphanus sativus L.) by non-radioactive AFLP analysis. Euphytica 129, 61–68 (2003). https://doi.org/10.1023/A:1021513331079

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