Behavior Genetics

, Volume 29, Issue 1, pp 47–57 | Cite as

Selective Breeding for High and Low Alcohol Preference in Mice

  • Nicholas J. Grahame
  • T.-K. Li
  • Lawrence Lumeng


High and low alcohol preference (HAP and LAP, respectively) mice were created by 10 generations of bidirectional selection for differences in two-bottle choice alcohol consumption. The progenitors used for selection were HS/lbg mice, which are a genetically defined, out-bred stock. During selection, mice had 24-h, daily access to 10% alcohol (v/v) and water ad libitum for 30 days and were selected based on the alcohol (g/kg) consumed per day over the entire period. Food was available ad libitum. At S10, line means for alcohol consumption differed greatly, with consumption of over 12 g/kg per day in the HAP mice and less than 2 g/kg per day in the LAP mice. Realized heritability for bidirectional selection was approximately 0.2. Female mice consumed more alcohol than male mice. There were no differences between lines in alcohol elimination rate, nor were there line differences in intake of salt or quinine solutions. However, consumption of saccharin solutions was greater in HAP mice than LAP mice, consistent with previous findings of a genetic correlation between sweet preference and alcohol drinking. Because the mouse genome is relatively well characterized, these selected lines should prove a useful tool for assessment of the genetic basis of, and phenotypes that correlate with, alcohol drinking.

Alcohol preference selective breeding taste alcohol metabolism heritability genetic differences 


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Copyright information

© Plenum Publishing Corporation 1999

Authors and Affiliations

  • Nicholas J. Grahame
    • 1
    • 2
  • T.-K. Li
    • 1
    • 2
  • Lawrence Lumeng
    • 1
  1. 1.Department of MedicineIndiana University School of Medicine and the Richard Roudebush Veterans Administration Medical CenterIndianapolisIndiana
  2. 2.Department of MedicineDiv. GIIndianapolisIndiana 46202

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