Abstract
The carboxylation of 2-oxoglutarate in the reductive tricarboxylic acid cycle in the obligate photolithotroph Chlorobium limicola forma thiosulfatophilum and the oxidation of isocitrate in the tricarboxylic acid cycle in the photoheterotroph Rhodopseudomonas palustris are catalyzed by isocitrate dehydrogenases. A comparative study of these enzymes isolated from the two bacteria showed that they virtually do not differ in enzymatic and kinetic properties.
Similar content being viewed by others
REFERENCES
Evans, M.C.W., Buchanan, V.V., Arnon, D.J., A New Ferredoxin-Dependent Carbon Reduction Cycle in a Photosynthetic Bacterium, Proc. Natl. Acad. Sci. USA, 1966, vol. 55, pp. 928–934.
Ivanovsky, R.N., Sintsov, N.V., and Kondratieva, E.N., ATP-linked Citrate Lyase Activity in the Green Sulfur Bacterium Chlorobium limicola forma thiosulfatophilum, Arch. Microbiol., 1980, vol. 128, pp. 239–241.
Schauder, R., Widdel, F., and Fuchs, G., Carbon Assimilation Pathways in the Sulfate-reducing Bacteria: II. Enzymes of a Reductive Citric Acid Cycle in the Autotrophic Desulfobacter hydrogenophilus, Arch. Microbiol., 1987, vol. 148, pp. 218–225.
Shiba, H., Kawasumi T., Igarashi Y., Kodoma T., Minoda, Y., The CO2 Assimilation via the Reductive Tricarboxylic Acid Cycle in an Obligately Autotrophic Aerobic Hydrogen-oxidizing Bacterium Hydrogenobacter thermophilus, Arch. Microbiol., 1985, vol. 142, pp. 198–203.
Ormerod, J.G., Ormerod, K.S., and Gest, H., Light-Dependent Utilization of Organic Compounds and Photoproduction of Molecular Hydrogen by Photosynthetic Bacteria: Relationships with Nitrogen Metabolism, Arch. Biochem. Biophys., 1961, vol. 94, pp. 449–463.
Larsen, H.J., On the Culture and General Physiology of the Green Sulfur Bacteria, J. Bacteriol., 1952, vol. 64, pp. 187–196.
Tulchin, N., Ornstein, L., and Davis, B.J., A Microgel System for Disc Electrophoresis, Anal. Biochem., 1976, vol. 72, pp. 485–490.
Garnak, M. and Reeves, H.C., Purification and Properties of Phosphorylated Isocitrate Dehydrogenase of Escherichia coli, J. Biol. Chem., 1979, vol. 254, pp. 7915–7920.
Bradford, M.M., A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding, Anal. Biochem., 1976, vol. 72, pp. 248–254.
Nimmo, H.G., Kinetic Mechanism of Escherichia coli Isocitrate Dehydrogenase and Its Inhibition by Glyoxylate and Oxaloacetate, Biochem. J., 1986, vol. 234, pp. 317–323.
Ruffo, A., Testa, E., Adinolfi, A., Pelizza, G., and Moratti, R., Control of the Citric Acid Cycle by Glyoxylate: Mechanism of the Inhibition by Oxalomalate and γ-Hydro-α-Oxoglutarate, Biochem. J., 1967, vol. 103, pp. 19–23.
Nimmo, H.G. and Craig, J.S., Structure and Regulation of Escherichia coli Isocitrate Dehydrogenase, Biochem. Soc. Trans., 1989, vol. 17, pp. 311–313.
Thauer, R.K., Jungermann, K., and Decker, K., Energy Conservation in Chemotrophic Anaerobic Bacteria, Bacteriol. Rev., 1977, vol. 41, pp. 100–180.
Reeves, H.C., Daumi, G.O., Lin, C.C., and Houston, M., NADP+-Specific Isocitrate Dehydrogenase of Escherichia coli: Purification and Characterization, Biochim. Biophys. Acta, 1972, vol. 258, pp. 27–39.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lebedeva, N.V., Malinina, N.V. & Ivanovsky, R.N. A Comparative Study of the Isocitrate Dehydrogenases of Chlorobium limicola forma thiosulfatophilum and Rhodopseudomonas palustris. Microbiology 71, 657–662 (2002). https://doi.org/10.1023/A:1021471621183
Issue Date:
DOI: https://doi.org/10.1023/A:1021471621183