Skip to main content
Log in

A Comparative Study of the Isocitrate Dehydrogenases of Chlorobium limicola forma thiosulfatophilum and Rhodopseudomonas palustris

  • Published:
Microbiology Aims and scope Submit manuscript

Abstract

The carboxylation of 2-oxoglutarate in the reductive tricarboxylic acid cycle in the obligate photolithotroph Chlorobium limicola forma thiosulfatophilum and the oxidation of isocitrate in the tricarboxylic acid cycle in the photoheterotroph Rhodopseudomonas palustris are catalyzed by isocitrate dehydrogenases. A comparative study of these enzymes isolated from the two bacteria showed that they virtually do not differ in enzymatic and kinetic properties.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

REFERENCES

  1. Evans, M.C.W., Buchanan, V.V., Arnon, D.J., A New Ferredoxin-Dependent Carbon Reduction Cycle in a Photosynthetic Bacterium, Proc. Natl. Acad. Sci. USA, 1966, vol. 55, pp. 928–934.

    Google Scholar 

  2. Ivanovsky, R.N., Sintsov, N.V., and Kondratieva, E.N., ATP-linked Citrate Lyase Activity in the Green Sulfur Bacterium Chlorobium limicola forma thiosulfatophilum, Arch. Microbiol., 1980, vol. 128, pp. 239–241.

    Google Scholar 

  3. Schauder, R., Widdel, F., and Fuchs, G., Carbon Assimilation Pathways in the Sulfate-reducing Bacteria: II. Enzymes of a Reductive Citric Acid Cycle in the Autotrophic Desulfobacter hydrogenophilus, Arch. Microbiol., 1987, vol. 148, pp. 218–225.

    Google Scholar 

  4. Shiba, H., Kawasumi T., Igarashi Y., Kodoma T., Minoda, Y., The CO2 Assimilation via the Reductive Tricarboxylic Acid Cycle in an Obligately Autotrophic Aerobic Hydrogen-oxidizing Bacterium Hydrogenobacter thermophilus, Arch. Microbiol., 1985, vol. 142, pp. 198–203.

    Google Scholar 

  5. Ormerod, J.G., Ormerod, K.S., and Gest, H., Light-Dependent Utilization of Organic Compounds and Photoproduction of Molecular Hydrogen by Photosynthetic Bacteria: Relationships with Nitrogen Metabolism, Arch. Biochem. Biophys., 1961, vol. 94, pp. 449–463.

    Google Scholar 

  6. Larsen, H.J., On the Culture and General Physiology of the Green Sulfur Bacteria, J. Bacteriol., 1952, vol. 64, pp. 187–196.

    Google Scholar 

  7. Tulchin, N., Ornstein, L., and Davis, B.J., A Microgel System for Disc Electrophoresis, Anal. Biochem., 1976, vol. 72, pp. 485–490.

    Google Scholar 

  8. Garnak, M. and Reeves, H.C., Purification and Properties of Phosphorylated Isocitrate Dehydrogenase of Escherichia coli, J. Biol. Chem., 1979, vol. 254, pp. 7915–7920.

    Google Scholar 

  9. Bradford, M.M., A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding, Anal. Biochem., 1976, vol. 72, pp. 248–254.

    Google Scholar 

  10. Nimmo, H.G., Kinetic Mechanism of Escherichia coli Isocitrate Dehydrogenase and Its Inhibition by Glyoxylate and Oxaloacetate, Biochem. J., 1986, vol. 234, pp. 317–323.

    Google Scholar 

  11. Ruffo, A., Testa, E., Adinolfi, A., Pelizza, G., and Moratti, R., Control of the Citric Acid Cycle by Glyoxylate: Mechanism of the Inhibition by Oxalomalate and γ-Hydro-α-Oxoglutarate, Biochem. J., 1967, vol. 103, pp. 19–23.

    Google Scholar 

  12. Nimmo, H.G. and Craig, J.S., Structure and Regulation of Escherichia coli Isocitrate Dehydrogenase, Biochem. Soc. Trans., 1989, vol. 17, pp. 311–313.

    Google Scholar 

  13. Thauer, R.K., Jungermann, K., and Decker, K., Energy Conservation in Chemotrophic Anaerobic Bacteria, Bacteriol. Rev., 1977, vol. 41, pp. 100–180.

    Google Scholar 

  14. Reeves, H.C., Daumi, G.O., Lin, C.C., and Houston, M., NADP+-Specific Isocitrate Dehydrogenase of Escherichia coli: Purification and Characterization, Biochim. Biophys. Acta, 1972, vol. 258, pp. 27–39.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lebedeva, N.V., Malinina, N.V. & Ivanovsky, R.N. A Comparative Study of the Isocitrate Dehydrogenases of Chlorobium limicola forma thiosulfatophilum and Rhodopseudomonas palustris. Microbiology 71, 657–662 (2002). https://doi.org/10.1023/A:1021471621183

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1021471621183

Navigation