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Dormant Cells in the Developmental Cycle of Blakeslea trispora: Distinct Patterns of the Lipid and Carbohydrate Composition

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Abstract

We revealed differences in lipid and carbohydrate composition between cells of mucorous fungi during endogenous and exogenous dormancy. Endogenous dormancy (zygospores) is characterized by high contents of phosphatidylcholine (about 70% of the total phospholipids) and triacylglycerol (over 90% of the total neutral lipids). By contrast, exogenous dormancy (sporangiospores) is accompanied by elevated amounts of sterols, sterol esters, and free fatty acids, which account for over 70% of the total neutral lipids. We established for the first time significant differences in the phospholipid composition between sporangiospores obtained from stylosporangia and sporangioles. Based on the data obtained, we regard the retardation of life-sustaining activities as a biochemical adaptation based on the dormancy state. We also discuss the taxonomical position of Blakeslea trispora.

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REFERENCES

  1. Blakeslee, A.F. and Cartledge, J.L., Sexual Dimorphism in Mucorales, Bot. Gaz., 1927, no. 74, pp. 51–53.

  2. Feofilova, E.P., Tereshina, V.M., and Memorskaya, A.S., Hetorothallism in Mucorous Fungi: Physiological and Biochemical Characterization of the (+) and (-) Strains of Blakeslea trispora, Mikrobiologiya, 1997, vol. 66, no.6, pp. 835–839.

    Google Scholar 

  3. Ganger, W.L., The Germination of Zygospores of Rhizopus stolonifer, Am. J. Bot., 1961, vol. 48, no. 5, pp. 427–429.

    Google Scholar 

  4. Feofilova, E.P., Tereshina, V.M., Memorskaya, A.S., and Khokhlova, N.S., Different Mechanisms of the Biochemical Adaptation of Mycelial Fungi to Temperature Stress: Changes in the Cytosol Carbohydrate Composition, Mikrobiologiya, 2000, vol. 69, no. 5, pp. 597–605.

    Google Scholar 

  5. Feofilova, E.P., Tereshina, V.M., Memorskaya, A.S., and Khokhlova, N.S., Different Mechanisms of the Biochemical Adaptation of Mycelial Fungi to Temperature Stress: Changes in the Lipid Composition, Mikrobiologiya, 2000, vol. 69, no. 5, pp. 606–612.

    Google Scholar 

  6. Folch, J., Lees, M., and Sloane-Stanlet, G.H.S., J. Biol. Chem., 1957, vol. 226, no. 1, pp. 497–529.

    Google Scholar 

  7. Kates, M., Techniques of Lipidology. Isolation, Analysis and Identification of Lipids, Amsterdam: Elsevier, 1972. Translated under the title Tekhnika lipidologii, Moscow, Mir, 1975.

    Google Scholar 

  8. Vakulova, L.A., Kuznetsova, V.P., Kolot, F.B., Bab'eva, I.P., and Samokhvalov, G.I., A Rapid Method for Quantitative Determination of β-Carotene in Microorganisms, Mikrobiologiya, 1964, vol. 33, no. 6, p. 1061.

    Google Scholar 

  9. Foppen, F.H., Tables for the Identification of Carotenoid Pigments, Chromatograph. Rev., 1971, vol. 14, pp. 133–298.

    Google Scholar 

  10. Brobst, K.M., in Metody issledovaniya uglevodov (Methods of Carbohydrates Research), Khorlin, F.Ya., Ed., Moscow: Mir, pp. 9–13.

  11. Ashmarin, I.P. and Vorob'ev, A.A., Statisticheskie metody v mikrobiologicheskikh issledovaniyakh (Statistical Methods in Microbiological Investigations), Leningrad: Gos. Izd-vo Med. Lit., 1962.

    Google Scholar 

  12. Feofilova, E.P., Peculiarities of Secondary Metabolism of Microorganisms as Related to the Process of Pigment Formation, Doctoral (Biol.) Dissertation, Moscow: Inst. Microbiol., Acad. Sci. USSR, 1977.

    Google Scholar 

  13. Martin, H.D., Ruck, C., Schmidt, M., Seil, S., and Reutuer, S., et al., Chemistry of Carotenoid Oxidation and Free Radical Reaction, Pure Appl. Chem., 1990, vol. 71, no. 12, pp. 2253–2262.

    Google Scholar 

  14. Zinse, E., Paltauf, F., and Daum, G., Sterol Composition of Yeast Organelle Membrane and Subcellular Distribution of Enzymes Involved in Sterol Metabolism, J. Bacteriol., 1993, vol. 175, pp. 2853–2858.

    Google Scholar 

  15. Van Laere, A.J., Cyclic AMP, Phosphodiesterase, and Spore Activation in Phycomyces blakesleeanus, Experimental. Mycol., 1986, vol. 10, pp. 52–59.

    Google Scholar 

  16. Pisarevskaya, I.V., Changes in the Carbohydrate and Lipid Composition of Absidia coerulea Cell Wall as Related to the Reproduction Process, Cand. Sci. (Biol.) Dissertation, Moscow: Inst. Microbiol., Acad. Sci. USSR, 1985.

    Google Scholar 

  17. Tereshina, V.M., Comparative Study of the Composition and Structure of the Cell Wall of the Fungi of the Family Choanephoraceae during the Process of Differentiation, Cand. Sci. (Biol.) Dissertation, Moscow: Inst. Microbiol., Acad. Sci. USSR, 1983.

    Google Scholar 

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Authors and Affiliations

  1. Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia

    V. M. Tereshina, A. S. Memorskaya & E. P. Feofilova

  2. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, Moscow oblast, 142292, Russia

    G. A. Kochkina

Authors
  1. V. M. Tereshina
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  2. A. S. Memorskaya
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  3. G. A. Kochkina
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  4. E. P. Feofilova
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Tereshina, V.M., Memorskaya, A.S., Kochkina, G.A. et al. Dormant Cells in the Developmental Cycle of Blakeslea trispora: Distinct Patterns of the Lipid and Carbohydrate Composition. Microbiology 71, 684–689 (2002). https://doi.org/10.1023/A:1021432007070

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