Lactobacillus delbrueckii subsp. delbrueckii KCTC 1047, grown in de Man, Rogosa and Sharpe (MRS) or soymilk media, completely hydrolyzed the isoflavone glucosides, genistin and daidzin at 50 μg ml−1, into their respective aglycones, genistein and daidzein within 30 min. Other lactic acid bacteria did not produce β-glucosidase, the enzyme responsible for the hydrolysis of isoflavone glucosides, when cultured in MRS medium. Glucoside-hydrolyzing activity was induced in some lactic acid bacteria when cultured in soymilk medium. These strains hydrolyzed 70–80% of genistin into genistein and 25–40% of daidzin into daidzein.
This is a preview of subscription content,to check access.
Access this article
Similar content being viewed by others
Adlercreutz H (1995) Phytoestrogens: epidermiology and a possible role in cancer protection. Environ. Health Perspect. 103: 103–112.
Adlercreutz H, Mousavi Y, Clark J, Hockerstedt K, Hamalainen E, Wahala K, Makela T, Hase T (1992) Dietary phytoestrogens and cancer: in vitro and in vivo studies. J. Steroid Biochem. Mol. Biol. 41: 331–337.
Akiyama T, Ishida J, Nakagawa S, Ogawara H, Watanabe S, Itoh NM, Shibuya M, Fukami Y (1987) Genistein a specific inhibitor of tyrosine specific protein kinases. J. Biol. Chem. 262: 5592–5595.
Axelson M, Setchell KDR (1981) The excretion of lignans in rats: evidence for an intestinal bacterial source for this new group of compounds. FEBS Lett. 123: 337–342.
Hutchins AM, Slavin JL, Lampe JW (1995) Urinary isoflavonoid phytoestrogen and lignan excretion after consumption of fermented and unfermented soy products. J. Am. Diet Assoc. 95: 545–551.
Izumi T, Piskula MK, Osawa S, Obata A, Tobe K, Saito M, Kataoka S, Kubota Y, Kikuchi M (2000) Soy isoflavone aglycones are absorbed faster and in higher amounts than their glucosides in humans. J. Nutr. 130: 1695–1699.
King RA, Broadbent JL, Head RJ (1996) Absorption and excretion of the soy isoflavone genistein in rats. J. Nutr. 126: 176–182.
Kiyosawa I, Matsuyama J, Arai C, Setoguchi T (1995) Suppressive effects of the methanol extracts from soybean products on SOS response of Salmonella typhimurium induced by mutagens and their contents of isoflavones. Nippon Shokuhin Kagaku Kaishi 42: 835–842.
Kurzer MS, Xu X (1997) Dietary phytoestrogen. Annu. Rev. Nutr. 17: 353–381.
Matsuda S, Norimoto F, Matsumoto Y, Ohba R, Teramoto Y, Ohta N, Ueda S (1994) Solubilization of a novel isoflavone glucoside-hydrolyzing ?-glucosidase from Lactobacillus casei subsp. rhamnosus. J. Ferment. Bioeng. 77: 439–441.
Matsuura M, Obata A (1993) ?-Glucosidases from soybeans hydrolyze daidzin and genistin. J. Food Sci. 58: 144–147.
Matsuura M, Sasaki J, Murao S (1995) Studies on ?-glucosidases from soybeans that hydrolyze daidzin and genistin: isolation and characterization of an isozyme. Biosci. Biotech. Biochem. 59: 1623–1627.
Potter SM (1995) Overview of proposed mechanisms for the hypocholesterolemic effect of soy. J. Nutr. 125: 606–611.
Wang G, Kuan SS, Francis OJ, Ware GM, Carman AS (1990) A simplified HPLCmethod for the determination of phytoestrogens in soybean and its processed products. J. Agric. Food Chem. 38: 185–190.
About this article
Cite this article
Choi, YB., Kim, KS. & Rhee, JS. Hydrolysis of soybean isoflavone glucosides by lactic acid bacteria. Biotechnology Letters 24, 2113–2116 (2002). https://doi.org/10.1023/A:1021390120400