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Is midazolam effective as an antioxidant in preventing reperfusion injury in rat kidney?

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Abstract

This experimental study was designed to investigate whether midazolam hasantioxidant effects in reperfused rat kidneys following ischemia. TwentyWistar Albino rats were included in the study. Rats were anesthetized withthe mixture of ketamine 90 mg/kg and xylazine 10 mg/kg administeredintraperitoneally. Following anesthesia, the rats were divided into twogroups. The first group was considered as the control group, whereas thesecond group received additional midazolam 3.5 mg/kg intraperitoneally.The left kidney was approached via a transabdominal incision and the leftrenal artery was dissected. Left renal ischemia was created by clamping theleft renal artery for 45 minutes. Following the ischemia period, the kidneywas reperfused for one hour. Both kidneys were then removed. Half of theleft kidneys were immediately immersed in liquid nitrogen fortransportation and then frozen at −70 C until measurements of tissuemalondialdehyde (MDA) and glutathione (GSH) levels. The remaining halvesof the left kidneys and right kidneys were fixed in 10% formalin. Thechanges which developed during the ischemia-reperfusion period in theleft kidney were investigated by histopathological examination andcompared with those of the normal contralateral kidney.When compared with the control group, tissue MDA and GSH levels weresimilar in the midazolam group (p > 0.05). Tubular damage with tubulitis andfocal interstitial inflammatory infiltration were observed inhistopathological examinations of reperfused left kidneys of the controlgroup. There was PMNL infiltration only in perirenal fat tissue of themidazolam group. Right kidneys were histopathologically normal in bothgroups.We concluded that within this dosage midazolam does not have anyantioxidant effect in reperfused rat kidneys following ischemia.

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Authors and Affiliations

  1. Department of Anesthesiology and Reanimation, University of Marmara, School of Medicine, Istanbul, Turkey

    Ugur Erol

  2. Department of Urology, Haydarpasa Numune Education and Research Hospital, Istanbul, Turkey (author for correspondence: Phone

    Mesut Gurdal & Ruknettin Aslan

  3. Department of Urology, University of Abant Izzet Baysal, Scholl of Medicine, Düzce, Turkey

    Ali Erol

  4. Department of Biochemistry, University of Istanbul Cerrahpasa, School of Medicine, Istanbul, Turkey

    Dildar Konukoğlu

  5. Department of Pathology, University of İnönü, School of Medicine, Malatya, Turkey

    Hale Onmus

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  1. Ugur Erol
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  2. Mesut Gurdal
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  3. Ali Erol
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Correspondence to Mesut Gurdal.

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Erol, U., Gurdal, M., Erol, A. et al. Is midazolam effective as an antioxidant in preventing reperfusion injury in rat kidney?. Int Urol Nephrol 34, 121–127 (2002). https://doi.org/10.1023/A:1021338806558

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