Skip to main content
SpringerLink
Log in

The effect of nitric oxide in testicular ischemia-reperfusion injury

  • Published:
International Urology and Nephrology Aims and scope Submit manuscript

Abstract

This experiment was carried out to investigate the effect of endogenous nitric oxide (NO) on the ischemia-reperfusion injury of testis.Testicular ischemia was achieved by twisting the right testis and spermatic cord 1080 counter-clockwise for 30 minutes and reperfusion was allowed for 30 minutes after detorsion of 33 rats. Animals were treated with normal saline in controls just before detorsion,NG-nitro-L-arginine methyl ester (L-NAME), and L-arginine (L-arg) in others.The tissue damage was evaluated with light microscopy, malondialdehyde (MDA) level in tissue, and the blood flow measurement using 133xenon (Xe) clearence technique.MDA indicator of reperfusion injury increased 25% after detorsion when only normal saline was given, L-NAME further increased MDA, L-arginin decreased MDA to control level.Conclusion: L-arginin infusion during the detorsion reduced the reperfusion injury of testis and improved the testicular blood flow after the detorsion.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Akgur FM, Kilinc K, Tanyel FC et al. Ipsilateral and contralateral testicular biochemical acute changes after unilateral testicular torsion and detorsion. Urology 1994; 44: 413.

    Google Scholar 

  2. Aydin S, Ugras S, Odabas O et al. Experimental testicular torsion and its effects on the contralateral testicle. Int Urol Nephrol 1997; 29: 661.

    Google Scholar 

  3. Becker EJ, Prillaman HM, Turner TT. Microvascular blood flow is altered after repair of testicular torsion in the rat. J Urol 1997; 157: 1493.

    Google Scholar 

  4. Bergh A, Damber JE, Marklund SE. Morphological changes induced by short term ischemia in rat testis are not affected by treatment with superoxide dismutase or catalase. J Androl 1988; 9: 15.

    Google Scholar 

  5. Carden DL, Granger DN. Pathophysiology of ischemia-reperfusioninjury. Jpathol 2000; 190: 255.

    Google Scholar 

  6. Ciftci AO, Muftuoglu S, Cakar N, Tanyel FC. Histological evidence of decreased contralateral testicular blood flow during ipsilateral testicular torsion. Br J Urol 1997; 80: 783.

    Google Scholar 

  7. Ferguson ND, Granton JT. Inhaled nitric oxide for hypoexemic respiratory failure: passing bad gas? C M A J 2000; 162: 85.

    Google Scholar 

  8. Janetschek G, Schreckenberg F, Grimm W, Marberger M. Hemodynamic effects of experimental testicular torsion. Urol Res 1987; 15: 303.

    Google Scholar 

  9. Kannan MS, Guiang S, Johnson DE. Nitric oxide: biological role and clinical uses. Indian J Pediatr 1998; 65: 333.

    Google Scholar 

  10. Kizilcan F, Bernay I, Tanyel FC et al. Ipsilateral and contralateral testicular blood flows during unilateral testicular torsion by 133Xe clearance technique. Int Urol Nephrol 1992; 24: 515.

    Google Scholar 

  11. Kosar A, Sarica K, Kupeli B et al. Testicular torsion: evaluation of contralateral testicular histology. Inti Urol Nephrol 1997; 29: 351.

    Google Scholar 

  12. Kubes P. The role of adhesion molecules nad nitric oxide in intestinal and hepatic ischemia-reperfusion. Hepatogastroenterology 1999; 46: 1458.

    Google Scholar 

  13. Mikuz G. Testicular torsion: simple grading for histological evaluation of tissue damage. Appl Pathol 1985; 3: 134.

    Google Scholar 

  14. Palmer JS, Plzak LF, Cromie WJ. Comparison of blood flow and histological changes in rat models of testicular ischemia. J Urol 1997; 158: 1138.

    Google Scholar 

  15. Prillaman HM, Turner TT. Rescue of testicular function after acute experimental torsion. J Urol 1997; 157: 340.

    Google Scholar 

  16. Saba M, Morales CR, De Lamirande E, Gagnon C. Morphological and biochemical changes following acute unilateral testicular torsion in prepubertal rats. J Urol 1997; 157: 1149.

    Google Scholar 

  17. Salman AB, Kilinc K, Tanyel FC. Torsion of only spermatic cord in the absence of testis and/epididymis results incontralateral testicular hypoxia. UrolRes 1997; 25: 413.

    Google Scholar 

  18. Schoenberg MH, Beger HG. Reperfusion injury after intestinal ischemia. Crit Care Med 1993; 21: 1376.

    Google Scholar 

  19. Tanyel FC, Buyukpamukcu N, Hicsonmez A. Contralateral testicular blood flow during unilateral testicular torsion. Br J Urol 1989; 63: 522.

    Google Scholar 

  20. Wax SH. Measurement of testicular blood flow by percutaneous injection of 133 Xe. Invest Urol 1971; 9: 167.

    Google Scholar 

  21. Weinbroum AA, Kluger Y, Shapira I, Rudick V. Methylene blue aboliches aortal tone impairment induced by liver ischemia-reperfusion in a dose response manner: an isolated perfused double-organ rat model study. Shock 2001; 15: 226.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pediatric Surgery, University of Ankara, School of Medicine, Ankara

    Meral Barlas & Celal Hatiboğlu

Authors
  1. Meral Barlas
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Celal Hatiboğlu
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Barlas, M., Hatiboğlu, C. The effect of nitric oxide in testicular ischemia-reperfusion injury. Int Urol Nephrol 34, 81–86 (2002). https://doi.org/10.1023/A:1021311029572

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1021311029572

Search

Navigation