Abstract
The isolate BOR-3, collected in Slovakia in 1996, was recently identified as a natural recombinant between an M and D type of Plum pox virus (PPV). Biological assays demonstrated its capacity to be aphid- and graft-transmitted to various Prunus spp. hosts. A study was carried out to determine the further presence of PPV recombinants in two epidemiologically distinct areas – Slovakia and France. Tools based on PPV-M and D subgroup typing, targeting P3–6K1, CI and CP regions of the PPV genome were used for recombinant identification. Closely related recombinant variants were detected in different Prunus spp. during a survey conducted in Slovakia in 2001, but not within a set of selected PPV isolates from France collected between 1985 and 2001. Sequence analysis of the (Cter)NIb–(Nter)CP region of 10 recombinant isolates from Slovakia showed their high homology, reaching more than 98%. All the recombinant isolates shared the same recombination breakpoint situated in the C terminus of the NIb gene. Our study demonstrates that the PPV recombinants are viable and competitive with conventional PPV-M and D isolates. The present work indicates that the occurrence of recombinants within PPV isolates might be more common than previously assumed.
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Aaziz R and Tepfer M (1999) Recombination in RNA viruses and in virus-resistant transgenic plants. Journal of GeneralVirology 80: 1339–1346
Allison RF, Janda M and Ahlquist P (1989) Sequence of cowpea chlorotic mottle virus RNAs 2 and 3 and evidence of a recombination event during bromovirus evolution. Virology 172: 321–330
Atreya PL, Lopez-Moya JJ, Chu M, Atreya CD and Pirone TP (1995) Mutational analysis of the coat protein N-terminal amino acids involved in potyvirus transmission by aphids. Journal of General Virology 76: 265–270
Bousalem M, Candresse T, Quiot-Douine L and Quiot JB (1994) Comparison of three methods for assessing plum pox virus variability: Further evidence for the existence of two major groups of isolates. Journal of Phytopathology 142: 163–172
Bousalem M, Douzery EJ and Fargette D (2000) High genetic diversity, distant phylogenetic relationships and intraspecies recombination events among natural populations of Yam mosaic virus: A contribution to understanding potyvirus evolution. Journal of General Virology 81: 243–255
Candresse T, Cambra M, Dallot S, Lanneau M, Asensio M, Gorris MT, Revers F, Macquaire G, Olmos A, Boscia D, Quiot JB and Dunez J (1998) Comparison of monoclonal antibodies and polymerase chain reaction assays for the typing of isolates belonging to the M and D serotypes of plum pox potyvirus. Phytopathology 88: 198–204
Cascone PJ, Haydar TF and Simon AE (1993) Sequences and structures required for recombination between virus-associated RNAs. Science 260: 801–805
Cervera MT, Riechmann JL, Martin MT and Garcia JA (1993) 3′terminal sequence of the plum pox virus PS and o6 isolates: Evidence for RNA recombination within the potyvirus group. Journal of General Virology 74: 329–334
Clark MF and Adams AN (1977) Characteristics of the microplate method of enzyme-linked immunosorbent assay for detection of plant viruses. Journal of General Virology 34: 475–483
Deborré G, Jelkmann W and Maiss E (1995) Biological and molecular biological investigations of several plum pox virus (PPV) isolates. Acta Horticulturae 386: 253–262
Devereux J, Haeberli P and Smithies O (1984) A comprehensive set of sequence analysis program for the VAX. Nucleic Acids Research 12: 387–395
Gibbs A and Mackenzie A (1997) A primer pair for amplifying part of the genome of all potyvirids by RT-PCR. Journal of Virological Methods 63: 9–16
Glasa M, Matisová J and Kúdela O (1998) Characterization of plum pox virus isolates from Slovakia. Acta Virologica 42: 226–229
Glasa M, Kúdela O, Marie-Jeanne V and Quiot JB (2001) Evidence of a naturally occurring recombinant isolate of Plum pox virus from Slovakia. Plant Disease 85: 920
Glasa M, Marie-Jeanne V, Moury B, Kúdela O and Quiot JB (2002) Molecular variability of the P3–6K1 genomic region among geographically and biologically distinct isolates of Plum pox virus. Archives of Virology 147: 563–575
Kölber M, Németh M, Chernets A, Kalashyan Y, Dulic-Markovic I, Glasa M, Isac M, Krška B, Malinowski T, Zawadzka B, Minoiu N, Myrta A, Navrátil M, Prichodko Y, Slováková L and Topchiiska M (2001) Current situation of plum pox disease on stone fruit species in Middle and Eastern Europe. Acta Horticulturae 550: 73–78
Labonne G, Yvon M, Quiot JB, Avinent L and Llacer G (1995) Aphids as potential vectors of plum pox virus: Comparison of methods of testing and epidemiological consequences. Acta Horticulturae 386: 207–216
Lai MMC (1992) RNA recombination in animal and plant viruses. Microbiological Reviews 56: 61–79
Lansac M, Chalak L, Cardona B, Sorbier A, Bodin-Ferri M, Dosba F, Labonne G, Quiot L and Quiot JB (1998) In vitro inoculation of Prunus species with plum pox potyvirus. Acta Horticulturae 472: 455–459
Le Gall O, Lanneau M, Candresse T and Dunez J (1995) The nucleotide sequence of the RNA-2 of an isolate of the English serotype of tomato black ring virus: RNA recombination in the history of nepoviruses. Journal of General Virology 76: 1279–1283
Mathews DH, Sabina J, Zuker M and Turner DH (1999) Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structure. Journal of Molecular Biology 288: 911–940
Myrta A, Di Terlizzi B, Boscia D, Caglayan K, Gavriel I, Ghanem G, Varveri C and Savino V (1998) Detection and serotyping of Mediterranean plum pox virus isolates by means of strain-specific monoclonal antibodies. Acta Virologica 42: 251–253
Nagy PD and Simon AE (1997) Newinsights into the mechanisms of RNA recombination. Virology 235: 1–9
Nemchinov L, Crescenzi A, Hadidi A, Piazzolla P and Verderevskaya T (1998) Present status of the new cherry subgroup of Plum pox virus (PPV-C). In: Hadidi A, Khetarpal RK and Koganezawa H (eds) Plant Virus Disease Control (pp 629–638) APS Press, St Paul, Minnesota
Nemchinov L, Hadidi A, Maiss E, Cambra M, Candresse T and Damsteegt V (1996) Sour cherry strain of plum pox potyvirus (PPV): Molecular and serological evidence for a new subgroup of PPV strains. Phytopathology 86: 1215–1221
Palkovics L, Burgyan J and Balazs E (1993) Comparative sequence analysis of four complete primary structure of plum pox virus strains. Virus Genes 7: 339–347
Quadt R and Jaspars EMJ (1989) RNA polymerases of plus-strand RNA viruses of plants. Molecular Plant-Microbe Interactions 2: 219–223
Revers F, Le Gall O, Candresse T, Le Romancer M and Dunez J (1996) Frequent occurrence of recombinant potyvirus isolates. Journal of General Virology 77: 1953–1965
Revers F, Le Gall O, Candresse T and Maule AJ (1999) New advances in understanding the molecular biology of plant/potyvirus interactions. Molecular Plant-Microbe Interactions 12: 367–376
Riechmann JL, Lain S and Garcia JA (1992) Highlights and prospects of potyvirus molecular biology. Journal of General Virology 73: 1–16
Roossinck MJ (1997) Mechanism of plant virus evolution. Annual Reviews of Phytopathology 35: 191–209
Simon AE and Bujarski JJ (1994) RNA-RNA recombination and evolution in virus-infected plants. Annual Reviews of Phytopathology 32: 337–362
Smith I, Candresse T and Dosba F (1994) La sharka, où en est-on en Europe et ailleurs? Arboriculture fruitiÉre 471: 29–34 and 52
Swanson MM and MacFarlane SA (1999) The E116 isolate of Dutch pea early-browning virus is a recombinant virus. Virus Research 60: 87–94
Szemes M, Kalman M, Myrta A, Boscia D, Németh M, Kölber M and Dorgai L (2001) Integrated RT-PCR/nested PCR diagnosis for differentiating between subgroups of plum pox virus. Journal of Virological Methods 92: 165–175
Šubr Z and Matisová J (1999) Preparation of diagnostic monoclonal antibodies against two potyviruses. Acta Virologica 43: 255–257
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F and Higgins DG (1997) The Clustal X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 24: 4876–4882
Weiller GF (1998) Phylogenetic profiles: A graphical method for detecting genetic recombinations in homologous sequences. Molecular Biology and Evolution 15: 326–335
Wetzel T, Candresse T, Ravelonandro M, Delbos RP, Mazyad H, Aboul-Ata AE and Dunez J (1991) Nucleotide sequence of the 3′-terminal region of the RNA of the El Amar strain of plum pox potyvirus. Journal of General Virology 72: 1741–1746
Wetzel T, Candresse T, Macquaire G, Ravelonandro M and Dunez J (1992) A highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection. Journal of Virological Methods 39: 27–37
Worobey M and Holmes EC (1999) Evolutionary aspects of recombination in RNA viruses. Journal of General Virology 80: 2535–2543
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Glasa, M., Marie-Jeanne, V., Labonne, G. et al. A Natural Population of Recombinant Plum Pox Virus is Viable and Competitive under Field Conditions. European Journal of Plant Pathology 108, 843–853 (2002). https://doi.org/10.1023/A:1021294221878
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DOI: https://doi.org/10.1023/A:1021294221878