Plant Cell, Tissue and Organ Culture

, Volume 72, Issue 1, pp 19–25 | Cite as

Induction of tetraploids in ornamental Alocasia through colchicine and oryzalin treatments

  • Nguyen Thi Phuong ThaoEmail author
  • Kenji Ureshino
  • Ikuo Miyajima
  • Yukio Ozaki
  • Hiroshi Okubo


Tetraploids were induced successfully from in vitro plants of diploid Alocasia `Green Velvet' (2n=28) by treating shoot tips with colchicine or oryzalin. Colchicine at a concentration of 0.01, 0.05, 0.1% and oryzalin at 0.005, 0.01, 0.05% (both were dissolved in 1% DMSO, dimethylsulfoxide) were tested for 24, 48 and 72 h. Ploidy levels could be easily determined by flow cytometry. In total, 396 plantlets were investigated. Controls with DMSO maintained the diploid status; 22 tetraploids and 22 chimeras were obtained from treatments with colchicine and oryzalin. The most efficient condition for inducing tetraploids seemed to be treatment with 0.01% oryzalin for 24 h. Variation in the morphological characteristic of leaf shape was found among the multiplied plants under the same growing condition. While the leaves of diploids had elongated-heart shape, the leaves of tetraploids and chimeras tended to be round.

Araceae chimera flow cytometry 


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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Nguyen Thi Phuong Thao
    • 1
    Email author
  • Kenji Ureshino
    • 1
  • Ikuo Miyajima
    • 1
  • Yukio Ozaki
    • 1
  • Hiroshi Okubo
    • 1
  1. 1.Laboratory of Horticultural Science, Faculty of AgricultureKyushu UniversityFukuokaJapan

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