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Influence of the culture medium constituents and inoculum size on the accumulation of blue pigment and cell growth of Lavandula spica

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Abstract

The effects of the concentration of PO4 3-, NO3 −, Fe 2+, sucrose and inoculum size on the accumulation of blue pigment and growth of suspension cultures of Lavandula spica D.C. (\( \equiv \) L. latifolia Vill.), were studied. The combination of 2.5 mM PO4 3-, 14.1 mM NO3 −, 1 mM Fe 2+, 30 g l−1 sucrose and 10 g l−1fresh weight of inoculum, promoted a 7-fold enhancement on the productivity of blue pigment in comparison to the control medium. Among all the culture medium constituents tested, phosphate exerted the highest beneficial effect and Fe2+ showed to be essential for the accumulation of the pigment. High levels of sucrose (60 or 90 g l−1) did not stimulate the accumulation of blue pigment. In these conditions, cell growth and cell viability were drastically affected.

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Authors and Affiliations

  1. Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, P.O. Box 24, Yautepec, Morelos, México, Z.C. 62731

    Gabriela Trejo-Tapia, Carlos Arias-Castro & Martha Rodríguez-Mendiola

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  1. Gabriela Trejo-Tapia
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  2. Carlos Arias-Castro
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  3. Martha Rodríguez-Mendiola
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Correspondence to Gabriela Trejo-Tapia.

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Trejo-Tapia, G., Arias-Castro, C. & Rodríguez-Mendiola, M. Influence of the culture medium constituents and inoculum size on the accumulation of blue pigment and cell growth of Lavandula spica . Plant Cell, Tissue and Organ Culture 72, 7–12 (2003). https://doi.org/10.1023/A:1021270907918

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  • DOI: https://doi.org/10.1023/A:1021270907918

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