Abstract
This study reports on the investigation of the polymorphism and geographical distribution of β-amylase isozymes by isoelectric-focusing (IEF) analysis in samples of world barley. The isozyme pattern of high thermostability type A and low thermostability type C varieties was restricted in isozyme type II. However, the isozyme pattern of the middle thermostability type B varieties was polymorphic. The β-amylase isozyme of 1,554 lines of the middle thermostability group was investigated next. Besides types I and II, new isozyme types III and IV were observed. Most lines were grouped into type II in all growing areas, except for Europe where type I and type II were even in number. Types III and IV were observed in only one line in India and Afghanistan, respectively. In Northern Europe, especially in Denmark, type I was major. It was concluded that a mutant IEF isozyme type I originated from the type II prototype of the thermostability B group in Northern Europe.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Eglinton J.K., Langridge P. and Evans D.E. 1998. Thermostability variation in alleles of barley beta-amylase. J. Cereal Science 28: 301–309.
Han F., Romagosa I., Ullrich S.E., Jones B.L., Hayes P.M. and Wesenburg D.M. 1997. Molecular marker assisted selection for malting quality traits in barley. Mol. Breed. 3: 427–437.
Kaneko T., Kihara M. and Ito K. 2000a. Genetic analysis of b-amylase thermostability to develop DNA marker for malt fermentability improvement in barley (Hordeum vulgare L.). Plant Breed. 119: 197–201.
Kaneko T., Kihara M., Ito K. and Takeda K. 2000b. Molecular and chemical analysis of b-amylase-less mutant barley in Tibet. Plant Breed. 119: 383–387.
Kaneko T., Zhang WS., Ito K. and Takeda K. 2001a. Worldwide distribution of b-amylase thermostability in barley. Euphytica 121: 223–228.
Kaneko T., Zhang WS., Takahashi H., Ito K. and Takeda K. 2001b. QTL Mapping for Enzyme Activity and Thermostability of b-Amylase in Barley (Hordeum vulgare L.). Breed. Sci. 51: 99–105.
Kihara M., Kaneko T. and Ito K. 1998. Genetic variation of b-amylase thermostability among varieties of barley (Hordeum vulgare L.) and relation to malting quality. Plant Breed. 117: 425–428.
Kihara M., Kaneko T., Ito K., Aida Y. and Takeda K. 1999. Geographical variation of b-amylase thermostability among varieties of barley (Hordeum vulgare L.) and b-amylase deficiency in barley. Plant Breed. 118: 453–455.
Nielsen G., Johansen H. and Jensen J. 1986. Proposal for the identification of barley varieties based on the genotypes for 2 hordein and 39 isoenzyme loci of 47 reference varieties. Euphytica 35: 717–728.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kaneko, T., Zhang, W., Ishii, M. et al. Differentiation and geographical distribution of β-amylase isozyme in barley. Genetic Resources and Crop Evolution 49, 599–605 (2002). https://doi.org/10.1023/A:1021256800893
Issue Date:
DOI: https://doi.org/10.1023/A:1021256800893