Skip to main content
Log in

Dynamic conservation of apricot Prunus armeniaca in saharian oases: use of AFLP markers to assess genetic diversity in traditional orchards

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

The genetic diversity of a traditional apricot (Prunus armeniaca) orchard in the Saharian oasis of El Golea was assessed using AFLP markers. We first showed that the technique can be used efficiently with Prunus armeniaca. Nineteen individuals sampled in three orchards were analyzed with seven primer combinations. A total of 197 bands were amplified, among which 97 revealed polymorphism. Genetic relationships among the individuals were assessed based on a Nei and Li's distance matrix using both the construction of a Neighbour Joining tree and an Analysis of Molecular Variance (AMOVA). The analyses show that there is no clear genetic structure between the orchards examined. These preliminary results are discussed in the view of implementing dynamic conservation programmes aimed at limiting the erosion of the genetic diversity of Saharian agrosystems.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Armstrong, J., A. Gibbs, R. Peakell & G. Weiller, 1994. The RAPDistance package. FTP://file.anu.edu.au/pub/software/RAPDistance.

  • Badenes, M.L., M.J. Asins, E.A. Carbonel & G. Glacer, 1996. Genetic diversity in apricot, Prunus armeniaca, aimed at improving resistance to plum pox virus. Plant Breed 115: 133–139.

    Article  CAS  Google Scholar 

  • Barbeau, G. & A. Elbouami, 1980. Prospections ‘Abricotier’ dans le Sud marocain. Fruits 35(2): 115–122.

    Google Scholar 

  • Bennett, M.D. & I.J. Leitch, 1995. Nuclear DNA amounts in angiosperms. Ann Bot 76: 113–176.

    Article  CAS  Google Scholar 

  • Cipriani, G., G. Lot, W.G. Huang, M.T. Marazzo, E. Peterlunger & R. Testolin, 1999. AC/GT and AG/CT microsatellite repeats in peach (Prunus persica (L.) Batsch): Isolation, characterization and cross-species amplification in Prunus. Theor Appl Genet 99: 65–72.

    Article  CAS  Google Scholar 

  • Clement Mullet, J.J., 1977. Le livre de l'agriculture, Ibn Al Awam, traduit de l'Arabe. Editions Bouslama, Tunis.

    Google Scholar 

  • Couranjou, J., J. Chapa, J.-M. Dufillol & H. Gall, 1975. Nouvelles variétés d'abricotier sélectionnées par l'Institut National de la Rechercher Agronomique. La Pomologie Franç aise 17(2): 29–37.

    Google Scholar 

  • Excoffier, L., P.E. Smouse & J.M. Quattro, 1992. Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131: 479–491.

    PubMed  CAS  Google Scholar 

  • Guénot, G., 1951. L'abricotier au Maroc. Fruits 6(11): 459–465.

    Google Scholar 

  • Hadrys, H., M. Balick & B. Schierwater, 1992. Applications of random amplified polymorphic DNA (RAPD) in molecular ecology. Mol Ecol 1: 55–63.

    PubMed  CAS  Google Scholar 

  • Jin, L.I. & R. Chakraborty, 1994. Population dynamics of DNA fingerprint patterns within and between populations. Genet Res Comb 63: 1–9.

    CAS  Google Scholar 

  • Le Thierry d'Ennequin, M., O. Panaud, B. Toupance & A. Sarr, 2000. Assessment of genetic relationships between Setaria italica and its wild relative S. viridis using AFLP markers. Theor Appl Genet 100(7): 1061–1066.

    Article  Google Scholar 

  • Loarce, Y., R. Gallego & E. Ferrer, 1996. A comparative analysis of the genetic relationships between rye cultivars using RFLP and RAPD markers. Euphytica 88: 107–115.

    Article  Google Scholar 

  • Lu, Z.-X., B. Sosinski, G.L. Reighard, W.V. Baird & A.G. Abbott, 1998. Construction of a genetic linkage map and identification of AFLP markers for resistance to root-knot nematodes in peach rootstocks. Genome 41: 199–207.

    Article  CAS  Google Scholar 

  • Mackill, D.J., Z. Zhang, E.D. Redona & P.M. Colowit, 1996. Level of polymorphism and genetic mapping of AFLP markers in rice. Genome 3: 969–977.

    Google Scholar 

  • Maughan, P.J., M.A. Saghai Maroof, G.R. Buss & G.M. Huestis, 1996. Amplified fragment length polymorphism (AFLP) in soybean: species diversity, inheritance, and near-isogenic line analysis. Theor Appl Genet 93: 392–401.

    Article  CAS  Google Scholar 

  • Milligan, B.G., J. Leebens-Mack & A.E. Strand, 1994. Conservation genetics: beyond the maintenance of marker diversity. Mol Ecol 3: 423–435.

    Google Scholar 

  • Nei, M. & W.H. Lit, 1979. Mathematical model for studying genetical variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 74: 5267–5273.

    Google Scholar 

  • Samer, F., 1992. Etude du pollen et de l'incompatibilité pollinique chez l'abricotier Prunus armeniaca. Application à la recherche de pollinisateurs et à la détermination de groupes d'intercompatibilité. D.A.A. Production végë tales. Ecole Nationale Supérieure Agronomique de Rennes, INRA (Station de Recherches Fruitiè res Méditérranéennes Montfavet Avignon.

    Google Scholar 

  • Tohme, J., D. Orlando Gonzales, S. Beebe & M.C. Duque, 1996. AFLP analysis of gene pools of a wild bean core collection. Crop Sci 36: 1375–1384.

    Article  CAS  Google Scholar 

  • Travis, S.E., J. Maschinski & P. Keim, 1996. An analysis of genetic variation in Astragalus cremnophylax var. cremnophylax, a critically endangered plant, using AFLP markers. Mol Ecol 5: 735–745.

    PubMed  CAS  Google Scholar 

  • Vos, P., R. Hogers, M. Bleeker, M. Reijans, T. van der Lee, M. Hornes, A. Frijters, J. Pot, J. Peleman, M. Kuiper & M. Zabeau, 1995. AFLP: a new technique for DNA fingerprinting. Nucl Acids Res 2: 4407–4414.

    Google Scholar 

  • Watkins, R., 1976. Cherry, plum, peach, apricot and almond. Prunus spp. (rosaceae). In: N.W. Simmonds (Ed.), Evolution of Crop Plants, pp. 242–247, Longman, London.

    Google Scholar 

  • Weber, J.L. & P.E. May, 1989. Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction. Am J Hum Genet 44: 388–396.

    PubMed  CAS  Google Scholar 

  • Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski & S.V. Tingey, 1990. DNA polymorphisms amplified by arbitrary primers and useful as genetic markers. Nucl Acids Res 18: 6531–6535.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Panaud, O., Chaib, A. & Sarr, A. Dynamic conservation of apricot Prunus armeniaca in saharian oases: use of AFLP markers to assess genetic diversity in traditional orchards. Euphytica 128, 301–305 (2002). https://doi.org/10.1023/A:1021247725148

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1021247725148

Navigation