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DNA Sequence-Specific Ligands: XI.* The Synthesis and Binding to DNA of bis-Netropsins with the C-Ends of Their Netropsin Fragments Tethered by Tetra- or Pentamethylene Linkers

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Abstract

Bis-Netropsins with the C-ends of their netropsin fragments tethered via tetra- or pentamethylene linkers and with Gly or L-Lys-Gly residues on their N-ends were synthesized. The footprinting technique was used to study the specificity of bis-netropsin binding to the specially constructed DNA fragments containing various clusters of A · T pairs. It was found that the linker length affects the binding of bis-netropsins, with the tetramethylene linker providing better protection than the pentamethylene linker. It was shown that the newly synthesized bis-netropsins bind tighter to the 5"-A 4 T 4-3" sequence, whereas the bis-netropsin with a linker between the netropsin N-ends binds better to 5"-T 4 A 4-3" sequences.

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Authors and Affiliations

  1. Moscow Center of Medical Research of Oslo University, ul. Vavilova 32, Moscow, 119991, Russia

    S. L. Grokhovsky

  2. Russian Academy of Sciences, Engelhardt Institute of Molecular Biology, ul. Vavilova 32, Moscow, 119991, Russia

    V. A. Nikolaev, B. P. Gottikh & A. L. Zhuze

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  1. S. L. Grokhovsky
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  2. V. A. Nikolaev
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  3. B. P. Gottikh
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  4. A. L. Zhuze
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Grokhovsky, S.L., Nikolaev, V.A., Gottikh, B.P. et al. DNA Sequence-Specific Ligands: XI.* The Synthesis and Binding to DNA of bis-Netropsins with the C-Ends of Their Netropsin Fragments Tethered by Tetra- or Pentamethylene Linkers. Russian Journal of Bioorganic Chemistry 28, 455–469 (2002). https://doi.org/10.1023/A:1021241515208

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