Abstract
The effect of two different dissolved oxygen (DO) concentrations (50 and 80%) on differentiation of somatic embryos (SE) from cell suspensions of coffee (Coffea arabica cv. Catimor 9722) was analyzed. Two bioreactors CMF-100 (CHEMAP AG) designed for the culture of cells, with 2-l glass vessels and a maximum work volume of 1.8 l were used. Each one was equipped with a gas blending unit (air, O2, N2, CO2) for the control of DO concentration. The inoculation density of embryogenic cells was 1.0 gram of fresh weight per liter (g FW l−1). The number of somatic embryos was greater (71 072 SE l−1) with 80% DO, but the major proportion were globular and heart shaped SE (66 399 SE l−1) and only 6.6% with regard to total was torpedo shaped SE. However, the 50% DO produced the higher number in the torpedo shaped SE (7389 SE l−1) what represented 20.0% with regard to total. Thus, higher concentrations of DO induced globular and heart shaped SE differentiation, but for production of torpedo shaped SE lower concentrations DO are needed. The somatic embryos obtained in the bioreactor with 50% DO showed similar behavior to the somatic embryos obtained in the rotary shaker. After 8 weeks of culture, 49.2% germination was obtained, which allowed a total of 1725 plantlet to be transferred to conditions ex vitro. After 6 months of culture, 89.2% of conversion was achieved and 1539 plants obtained were transferred to field conditions.
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References
Acuña R & de Peña M (1991) Plant regeneration from of embryogenic cell suspensions of Coffea arabica L. cv. Caturra. Plant Cell Rep. 10 345-348
Archambault J, Lavoie L, Williams RD & Chavarie C (1995) Nutritional aspects of Daucus carota somatic embryo cultures performed in bioreactors. In: Terzi M et al. (eds) Current Issues in Plant Molecular and Cellular Biology, (pp 681-687). Kluwer Academic Publishers, Dordrecht, The Netherlands
Berthouly M (1997) Biotecnologías y técnicas de reproducción de materiales promisorios en Coffea arabica (pp 25-49). XVIII Simposio Latinoamericano de Caficultura, Costa Rica
Carman JG (1988) Improved somatic embryogenesis in wheat by partial simulation of the in-ovulo oxygen, growth-regulator and dessication environments. Planta 175: 417-424
Cevallos AM (2000) Establecimiento de una metodología eficiente en el proceso de embriogénesis somática del cafeto (Coffea spp.), mediante el uso de marcadores morfohistológicos y moleculares. Tesis de doctorado. (pp 1-101). Instituto Nacional de Ciencias Agrícolas, La Habana, Cuba
Ducos JP, Z amarripa A, Eskes AB & Petiard V (1993) Production of somatic embryos of coffee in a bioreactor. 15th conference ASIC, Montpellier. pp 89–95
Etienne H, Solano W, Pereira A, Barry-Etienne D, Bertrand B, Anthony F, Côte F & Berthouly M (1997) Utilización de la embriogénesis somática en medio líquido para la propagación masal de los híbridos F1 de Coffea arabica (pp 253-261). XVIII Simposio Latinoamericano de Caficultura, Costa Rica
Hohe A, Winkelmann T & Schwenkel HG (1999) The effect of partial pressure in boreactors on cell proliferation and subsequent differentiation of somatic embryos of Cyclamen persicum. Plant Cell Tiss. Org. Cult. 59: 39-45
Jay V, Genestier S & Courduroux JC (1992) Bioreactor studies on the effect of dissolved oxygen concentrations on growth and differentiation of carrot (Daucus carota L.) cell cultures. Plant Cell Rep. 11: 605-608
Jay V, Genestier S & Courduroux JC (1994) Bioreactor studies of the effect of medium pH on carrot (Daucus carota L.) somatic embryogenesis. Plant Cell Tiss. Org. Cult. 36: 205-209
Jiménez E & de Feria M (1998) Empleo de Biorreactores para la propagación masiva. In: Pérez JN (ed) Propagación y Mejora Genética de Plantas por Biotecnología (pp 207-224)
Kvaalen H & von Arnold S (1991) Effects of various partial pressures of oxygen and carbon dioxide on different stages of somatic embryogenesis in Picea abies. Plant Cell Tiss. Org. Cult. 27: 49-57
Mavituna F & Buyukalaca S (1996) Somatic embryogenesis of pepper in bioreactors: a study of bioreactor type and oxygen-uptake rates. Appl. Microbiol. Biotechnol. 46 (4): 327-333
Merkle S, Parrott W & Flinn B (1995) Morphogenic aspects of somatic embryogenesis. In: Thorpe T (ed) In vitro Embryogenesis in Plants (pp 155-203)
Montes S, Martínez M, Rojas R, Santana N & Cuba M (1995) Obtención de embriones somáticos a partir de suspensiones celulares de Coffea canephora variedad Robusta. Cultivos Tropicales 16(3): 77-81
Murashige T & Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant 15: 473-497
Okamoto A, Kishine S, Hirosawa T & Nakazono A (1996) Effect of oxygen-enriched aeration on regeneration of rice (Oriza sativa L.) cell culture. Plant Cell Rep. 15: 731-736
Preil W (1991) Application of bioreactors in plant propagation. In: Debergh PC & Zimmerman RH (eds) Micropropagation (pp 425-445)
Preil W, Florek P, Wix U & Beck A (1988) Towards mass propagation by use of bioreactors. Acta Horticulturae 226: 99-106
Santana N, Cortés S, Martín JV & Montes S (1996) Adaptación de vitroplantas de embriones somáticos de cafeto (Coffea arabica L.) variedad Catimor (9722). Cultivos Tropicales 17(2): 83-85
Scragg AH (1995) The problems associated with high biomass levels in plant cell suspensions. Plant Cell Tiss. Org. Cult. 43(2): 163–170.
Shimazu T & Kurata Kenji (1999) Relationship between production of carrot somatic embryos and dissolved oxygen concentration in liquid culture. Plant Cell Tiss. Org. Cult. 57 29–38
Söndahl MR, Nakamura T & Sharp WR (1991) Propagación in vitro del café. In: Roca WM & Mroginski LA (eds) Cultivo de Tejidos en la Agricultura, Vol. 27 (pp 621-642)
Stuart DA & Strickland SG (1984) Embryogenesis from cell culture of Medicago sativa. The role of amonio acid additions to the regeneration medium. Plant Sci. Lett. 34:74
Stuart DA, Strickland SG & Walker KA (1987) Bioreactor production of alfalfa somatyc embryos. Hortscience 22: 800-803
Tahara M, Yasuda T, Uchinda N & Yamaguchi T (1994) Formation of somatic embryos from protoplasts of Coffea arabica. L Hortscience 29: 172-174
Teng WL, Lin CP & Liu AJ (1993) Regenerating lettuce from suspension culture in a 2-liter bioreactor. HortiScience 28: 669-671
Van Boxtel J & Berthouly M (1996) High frequency somatic embryogenesis from coffee leaves: factors influencing embryogenesis, and subsequent and regeneration in liquid medium. Plant Cell Tiss. Org. Cult. 44: 7-17
Vasil IK (1994) Automation of plant micropropagation. Plant Cell Tiss. Org. Cult. 39: 105-108
Vasquez N, Salazar K, Solano W, Pescira A, Bertand B & Etienne H (1998) Embriogénesis de alta frecuencia en híbridos F1 seleccionados de Coffea arabica a partir de explantes de hoja: reactividad y eventos histológicos. In: III Encuentro Latinoamericano de Biotecnología Vegetal, REDBIO 98. La Habana (1-5 Junio) (516 p)
Zamarripa A, Ducos JP, Tesserau H, Bollon H, Eskes AB & Petiard V (1991a) Developpement D'un procede de multiplication en masse du cafeier par embrygenese somatique en milieu liquide. ASIC. 14th conference, San Francisco, pp 392-402
Zamarripa A, Ducos JP, Bollon H, Dufom M & Petiard V (1991b) Production d'embryos somatiques de cafeier en milieu liquide: effects dente d'insulation et renourellement du milieu. Cafe Cacao Tfe, vol XXXV, no. 4, oct-dec. 1991
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de Feria, M., Jiménez, E., Barbón, R. et al. Effect of dissolved oxygen concentration on differentiation of somatic embryos of Coffea arabica cv. Catimor 9722. Plant Cell, Tissue and Organ Culture 72, 1–6 (2003). https://doi.org/10.1023/A:1021202305692
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DOI: https://doi.org/10.1023/A:1021202305692