Abstract
This study reports further information on “mareninne,” awater-soluble blue-green pigment synthesized by the diatom Hasleaostrearia, which is essential to the greening of maturing oysters inFrench production areas. The extraction process is reported, as well aspreliminary characterization of a partially purified marennine extract,including quantitative spectrophotometric analysis of intracellular pigment. Amean specific extinction coefficient, E 1% 1cm = 17.2 at 669 nm, is proposed. Results forquantitative determination of marennine accumulated in cells during batchcultures are presented, and their importance for pigment production isconsidered in relation to potential industrial applications.
Similar content being viewed by others
References
Bocat L. 1907. Sur la marennine de la diatomée bleue. Comparaison avec la phycocyanine. Comptes r. Soc. Biol. 62: 1073-1075.
Carbonnelle D., Pondaven P., Morançais M., Massé G., Bosch S., Jacquot C. et al. 1999. Antitumor and antiproliferative effects of an aqueous extract from the marine diatom Haslea ostrearia (Gaillon) Simonsen against solid tumors: lung carcinoma (NSCLC-N6), kidney carcinoma (E39) and melanoma (M 96) cell lines. Anticancer Res. 19: 621-624.
Génevès L., Choussy M., Barbier M., Neuville D. and Daste P. 1976. Ultrastructure et composition pigmentaire comparées des chromatophores de la diatomée Navicula ostrearia (Bory) normale et bleue. C. r. Acad. Sci., Paris 282: 449-452.
Guillard R.R.L. 1975. Culture of phytoplancton for feeding marine invertebrates. In: Smith W.L. and Chanley M.H. (eds), Culture of Marine Invertebrate Animals. Plenum Press, New York, pp. 26-60.
Lankester E. 1886. 0n green oysters. Quart. J. microsc. Sci. 26: 71-94.
Lebeau T., Junter G.-A., Jouenne T. and Robert J.-M. 1999. Marennine production by agar-entrapped Haslea ostraeria Simonsen. Biores. Technol. 67: 13-17.
Moreau J. 1970. Contributions aux recherches écologiques sur les claires à huîtres du bassin de Marennes-Oléron. PhD Dissertation, University of Nantes.
Mouget J.-L., Tremblin G., Morant-Manceau A., Morançais M. and Robert J.-M. 1999. Long-term photoacclimatization of Haslea ostrearia. (Bacilliariophyta): growth and pigment content, in relation with photosynthesis. Eur. J. Phycol. 34: 109-115.
Moreau C., Robert J.-M. and Rouillard I. 1994. Effect of iron and manganese on the growth of the diatom Haslea ostrearia in batch culture. Cah. Biol. mar. 35: 269-279.
Nassiri Y., Robert J.-M., Rincé Y. and Ginsburger-Vogel T. 1998. The cytoplasmic fine structure of the diatom Haslea ostrearia (Bacillariophyceae) in relation to marennine production. Phycologia 37: 84-91.
Neuville D. and Daste P.H. 1971. Variations structurales présentées par la diatomée Navicula ostrearia (Gaillon) Bory soumise a différentes conditions de culture. Comptes Rendusde la Société Biologique, Poitiers. 7: 1723-1726.
Ranson G. 1927. L'absorption de matières organiques dissoutes par la surface extérieure du corps chez les animaux aquatiques. Ann. Inst. Ocean. IV: 49-174.
Robert J.-M. 1974. Etude préliminaire des relations entre l'état de verdissement d'une claire ostréicole et l'examen biométrique de populations planctoniques et benthiques du Navicula ostrearia Bory, pigmenté; applications. C. r. Acad. Sci., Paris 278: 1405-1408.
Robert J.-M., Pages J. and Prat D. 1975. Applications de la biométrie cytologique à la définition des stades de développement de Navicula ostrearia (Bory): incidences de l'évolution pigmentaire sur le verdissement des claires à huîtres. Physiol. Vég. 13: 225-241.
Robert J.-M. and Hallet J.-N. 1981. Absorption spectrum in vivo of the blue pigment marennine of the pennate diatom Navicula ostrearia (Bory). J. Exp. Bot. 32: 341-345.
Robert J.-M. 1983. Fertilité des claires ostréicoles et verdissement: utilisation de l'azote par les diatomées dominantes. PhD Dissertation, University of Nantes.
Schubert H., Tremblin G., Robert J.-M., Sagert S. and Rincé Y. 1995. In vivo fluorescence measurement of photosynthesis of Haslea ostrearia Simonsen in relation to marennine content. Diatom. Res. 10: 341-349.
Tremblin G. and Robert J.-M. 1996. Comportement photosynthétique de Haslea ostrearia en relation avec sa pigmentation bleue. C. r. Acad. Sci. Paris 319: 939-944.
Tremblin G. and Robert J.-M. 2001. Carbon fixation by the peculiar marine diatom Haslea ostrearia. Photosynthetica 39: 215-220.
Tremblin G., Cannuel R., Mouget J.L., Rech M. and Robert J.-M. 2000. Change in light quality due to a blue-green pigment, marennine, released in oyster-ponds: effect on growth and photosynthesis in two diatoms, Haslea ostrearia and Skeletonema costatum. J. appl. Phycol. 12: 557-566.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Robert, JM., Morançais, M., Pradier, E. et al. Extraction and quantitative analysis of the blue-green pigment “marennine” synthesized by the diatom Haslea ostrearia . Journal of Applied Phycology 14, 299–305 (2002). https://doi.org/10.1023/A:1021184532581
Issue Date:
DOI: https://doi.org/10.1023/A:1021184532581