Abstract
Purpose: The present study assessed whether the poor semen quality in patients with cancer results from the inhibition of sperm maturation as indicated by creatine kinase or from increased oxidative stress as assessed by lipid peroxidation of the sperm membrane.
Methods: Cryopreserved semen specimens from patients with testicular (n = 10) and nontesticular (n = 12) cancer and normal healthy donors (n = 14) were analyzed for lipid peroxidation and creatine kinase levels.
Results: The levels of creatine kinase and malonaldehyde did not differ among testicular or nontesticular patients with cancer or normal healthy donors.
Conclusions: Poor semen quality in testicular and nontesticular patients with cancer is not related to creatine kinase or lipid peroxidation levels; it may be related to other factors.
Similar content being viewed by others
REFERENCES
Carter M: Fertility in the testicular cancer patient. World J Urol 1993;11:70–75
Myers SE, Schilsky RL: Prospects for fertility after cancer chemotherapy. Semin Oncol 1992;19:597–603
Carson SA, Gentry WL; Smith AL, Buster JE: Feasibility of semen collection and cryopreservation during chemotherapy. Hum Reprod 1991;6:992–994
Agarwal A, Loughlin KR: Acrosin activity in patients with idiopathic infertility. Arch Androl 1991;27:97–101
Aitken J, Krausz C, Buckingham D: Relationships between biochemical markers for residual sperm cytoplasm, reactive oxygen species generation, and the presence of leukocytes and precursor germ cells in human sperm suspensions. Mol Reprod Dev 1994;39:268–279
Huszar G, Vigue L, Corrales M: Sperm creatine kinase activity in fertile and infertile oligospermic men. J Androl 1990;11:40–46
Huszar G, Vigue L: Correlation between the rate of lipid peroxidation and cellular maturity as measured by creatine kinase activity in human spermatozoa. J Androl 1994;15:71–77
Tummon IS, Yuzpe AA, Daniel SA, Deutsch A: Total acrosin activity correlates with fertility potential after fertilization in vitro. Fertil Stertil 1991;56:933–938
Jones R, Mann T, Sherins R: Peroxidative breakdown of phospholipids in human spermatozoa, spermicidal properties of fatty acid peroxides, and protective action of seminal plasma. Fertil Stertil 1979;31:531–537
World Health Organization: WHO Laboratory Manual for the Examination of Human Semen and Semen-Cervical Mucus Interaction, 3rd ed. Cambridge, Cambridge University Press, 1992
Parinaud J, Richoilley G, Vieitez G, Labal B, Moutaffian H: Relevance of acrosome function in the evaluation of semen in vitro fertilizing ability. Fertil Stertil 1995;63:598–603
Agarwal A, Shekarriz M, Sidhu RS, Thomas AJ: Value of clinical diagnosis in predicting the quality of cryopreserved sperm from cancer patients. J Urol 1996;155:934–938
Bell M, Sikka SC, Rajasekaran M, Hellstrom WJG: Time course study of hydrogen peroxide induced changes in the lipid peroxidation of human sperm membranes. Assist Reprod Technol Androl 1992;5:144–150
Hellstrom WJ, Bell M, Wang R, Sikka SC: Effect of sodium nitroprusside on sperm motility, viability and lipid peroxidation. Fertil Steril 1994;61:1117–1122
Chapman RM, Sutcliffe SB, Rees LH, Edwards CR, Malpas JS: Cyclical combination chemotherapy and gonadal function. Retrospective study in males. Lancet 1979;1:285–289
Huszar G, Vigue L, Corrlaes M: Sperm creatine phosphokinase activity as a measure of sperm quality in normospermic, variablespermic, and oligospermic men. Biol Reprod 1988;38:1061–1066
Huszar G, Vigue L: Spermatogenesis-related change in the synthesis of the creatine kinase B-type and M-type isoforms in human spermatozoa. Mol Reprod Dev 1990;25:258–262
Huszar G, Vigue L, Morshedi M: Sperm creatine phosphokinase M-isoform ratios and fertilizing potential of men: a blinded study of 84 couples treated with in vitro fertilization. Fertil Stertil 1992;57:882–888
Alvarez JG, Storey BT: Evidence of increased lipid peroxidative damage and loss of superoxide dismutase activity as a mode of sublethal cryodamage to human sperm during cryopreservation. J Androl 1992;13:232–241
Irvine DS, Aitken RJ: Seminal fluid analysis and sperm function testing. Endocrinol Metab Clin North Am 1994;23:725–748
Agarwal A, Newton RA: Effect of cancer on semen quality after cryopreservation of sperm. Andrologia 1991;23:329–332
Aitken RJ: Pathophysiology of human spermatozoa. Curr Opin Obstet Gynecol 1994;6:128–135
Bell M, Wang R, Hellstrom WJ, Sikka SC: Effect of cryoprotective additives and cryopreservation protocol on sperm membrane lipid peroxidation and recovery of motile human sperm. J Androl 1993;14:472–478
Rao B, Soufir JC, Martin M, David G: Lipid peroxidation in human spermatozoa as related to midpiece abnormalities and motility. Gamete Res 1989;24:127–134
Agarwal A, Tolentino MV, Sidhu RS, Ayzman I, Lee J-C, Thomas AJ, Shekarriz M: Effect of cryopreservation on semen quality in patients with testicular cancer. Urology 1995;46:382–389
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sidhu, R.S., Wang, Y. & Agarwal, A. Creatine Kinase Level and Lipid Peroxidation Rate in Human Spermatozoa from Patients with Cancer. J Assist Reprod Genet 14, 538–542 (1997). https://doi.org/10.1023/A:1021183528047
Issue Date:
DOI: https://doi.org/10.1023/A:1021183528047