Abstract
The initial activity rate for the synthesis of geranyl butyrate by an esterase from Mucor miehei increased with increasing water activity and decreased above 0.5 water activity. The conversion yield after 75 h was about 75% for different, but low, initial water activities and decreased above 0.5 water activity. This behaviour is related to water-enzyme interactions.
Similar content being viewed by others
References
Bartling K, Thompson JUS, Pfromm PH, Czermak P, Rezac ME (2001) Lipase-catalyzed synthesis of geranyl acetate in n-hexane with membrane-mediated water removal. Biotechnol. Bioeng. 75: 676–681.
Borzeix F, Monot F, Vandecasteele JP (1992) Strategies for enzymatic esterification in organic solvents: comparison of microaqueous, biphasic, and micellar systems. Enzyme Microbiol. Technol. 14: 791–797.
de Castro HF, de Oliveira PC, Pereira EB (1997) Evaluation of different approaches for lipase catalysed synthesis of citronellyl acetate. Biotechnol. Lett. 19: 229–232.
Drapron R (1972) Réactions enzymatiques en milieu peu hydrate. Ann. Technol. Agric. 21: 487–499.
Gubicza L, Kabiri-Badr A, Keoves E, Belafi-Bako K (2000) Largescale enzymatic production of natural flavour esters in organic solvent with continuous water removal. J. Biotechnol. 847: 193–196.
Karra-Chaabouni M, Pulvin S, Touraud D, Thomas D (1996) Enzymatic synthesis of geraniol esters in a solvent-free system by lipases. Biotechnol. Lett. 18: 1083–1088.
Karra-Chaabouni M, Pulvin S, Touraud D, Thomas D (1998) Parameters affecting the synthesis of geranyl butyrate by esterase 30 000 from Mucor miehei. J. Am. Oil Chem. Soc. 75: 1201–1206.
Valivety R.H, Halling PJ, Macrae AR (1992) Reaction rate with suspended lipase catalysts shows similar dependence on water activity in different organic solvents. Biochim. Biophys. Acta 1118: 218–222.
Wehtje E & Adlercreutz P (1997a) Lipases have similar water activity profiles in different reactions. Biotechnol. Lett. 11: 537–540.
Wehtje E & Adlercreutz P (1997b) Water activity and substrate concentration effects on lipase activity. Biotechnol. Bioeng. 55: 798–806.
Wehtje E, Kaur J, Adlercreutz P, Chand S, Mattiasson B (1997) Water activity control in enzymatic esterification processes Enzyme Microbiol. Technol. 21: 502–510.
Zaks A, Klibanov AM (1988) The effect of water on enzyme action in organic media. J. Biol. Chem. 263: 8017–8021.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Karra-Chaabouni, M., Pulvin, S., Thomas, D. et al. Role of water activity on the synthesis of geranyl butyrate by a Mucor miehei esterase in a solvent-free system. Biotechnology Letters 24, 1951–1955 (2002). https://doi.org/10.1023/A:1021173615101
Issue Date:
DOI: https://doi.org/10.1023/A:1021173615101