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Occurrence of catecholaminergic nerve fibers in the human uveoscleral tissue in conditions of normal and raised intraocular pressure

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Abstract

The distribution of catecholaminergic nerve fibers (CNF) in human uveoscleral tissue was studied in six human eyes with normal intraocular pressure and in five eyes with increased pressure. The eyes with increased pressure had no visual field alterations and the patients did not have anyglaucoma-related opticneuropathies. The amount of norepinephrine in these structures was also analysed. Catecholaminergic nerve fibers were detected by means of fluorescence microscopy and were counted using the quantitative analysis of images. Our results demonstrate that the occurrence of catecholaminergic nerve fibers (expressed in Conventional Units = C.U.) in human uveoscleral tissue is 15.4 ± 1.6 C.U. in eyes with normal intraocular pressure. In eyes with increased intraocular pressure, these values were 12.2 ± 1.2 C.U. Moreover, the amount of norepinephrine in tissue homogenates of the same eyes was evaluated and found to be 21.7 ± 1.3 μg/gr tissue fresh weight of the human uveoscleral tissue in eyes with normal intraocular pressure. This value decreased to 18.8 ± 1.1 μg/gr tissue fresh weight in the same tissue in conditions of raised intraocular pressure. In these experiments, the small number of eyes examined made it difficult to draw general conclusions. However, the role of human uveoscleral tissue was emphasized by the rich catecholaminergic innervation. A decrease of catecholaminergic nerve fibers and norepinephrine occurs when intraocular pressure is elevated. The modifications of these parameters, involved in the sympathetic control of aqueous humor outflow, may support the hypothesis of a possible relevant role for the human uveoscleral tissue in different pathological conditions.

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Authors and Affiliations

  1. Department of Human Anatomy, University “La Sapienza”, Rome, Italy

    Carlo Cavallotti & Marco Artico

  2. Eye Clinic University “La Sapienza”, Rome, Italy

    Nicola Pescosolido & Elena Pacella

  3. 2nd Neurologic Clinic University “La Sapienza”, Rome, Italy

    Daniela Cavallotti

Authors
  1. Carlo Cavallotti
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  2. Nicola Pescosolido
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  3. Marco Artico
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  4. Elena Pacella
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  5. Daniela Cavallotti
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Cavallotti, C., Pescosolido, N., Artico, M. et al. Occurrence of catecholaminergic nerve fibers in the human uveoscleral tissue in conditions of normal and raised intraocular pressure. Int Ophthalmol 24, 133–139 (2001). https://doi.org/10.1023/A:1021173115883

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