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The Activities of Six Exo- and Endopeptidases in the Substantia Nigra, Neostriatum, and Cortex of the Rat Brain

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Abstract

We determined the enzymatic activity and crude subcellular distribution of four exopeptidases: Dipeptidylaminopeptidase IV (DAP-IV), Alanyl aminopeptidase (AAP), Prolyl aminopeptidase (PAP) and γ-Glutamyl transpeptidase (γGTP), and two endopeptidases: Postproline endopeptidase (PEP) and “Trypsin-like” peptidase (“T-L” P) in pars compacta (SNPC) and pars reticulata (SNPR) of substantia nigra, caudate-putamen (CAU) and cerebral cortex (CC) of the rat brain. We found: 1) DAP-IV activity is comparatively higher in SNPC and it is equally distributed in the postmitochondrial precipitate (PR) and supernatant (SN) fractions of SNPC, CAU and CC but higher in the SN from SNPR. 2) CC shows the highest activity of AAP and its activity is mainly located in the SN from all areas. 3) The activity of PAP is comparatively higher in SNPC and it is exclusively located in the SN from all areas. 4) γGTP activity is similar in all areas but its predominance is in the SN for SNPC and SNPR, and in the PR for CAU and CC. 5) CAU has higher PEP activity (higher in the PR) than CC (higher in the SN); no activity is detected in the substantia nigra. 6) The activity of a “Trypsin-like” peptidase is the highest in SNPC and SNPR; this activity have some predominance in the SN and higher predominance in the same fraction from CAU and CC.

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Authors and Affiliations

  1. Departamento de Farmacología y Toxicología, Mexico

    M. Elisabeth Hernández Gallegos, Ma. Magdalena Ibarra Zannatha, Eduardo García Osornio & Francisco A. Posadas del Rio

  2. Departamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y de Estudios Avanzados del IPN, Apartado Postal 14-740, 07000, México, D.F., Mexico

    Arturo Sierra Sánchez

  3. Departamento de Fisiología y Farmacología, Centro de Estudios Básicos, Universidad Autónoma de Aguascalientes, Aguascalientes, AGS, Mexico

    Francisco A. Posadas del Rio

Authors
  1. M. Elisabeth Hernández Gallegos
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  2. Ma. Magdalena Ibarra Zannatha
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  3. Eduardo García Osornio
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  4. Arturo Sierra Sánchez
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  5. Francisco A. Posadas del Rio
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Gallegos, M.E.H., Zannatha, M.M.I., Osornio, E.G. et al. The Activities of Six Exo- and Endopeptidases in the Substantia Nigra, Neostriatum, and Cortex of the Rat Brain. Neurochem Res 24, 1557–1561 (1999). https://doi.org/10.1023/A:1021156216661

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