Abstract
Hydrophilic benzo[b]thiophene metabolites produced by Rhodococcus sp. strain T09 were isolated by a solid-phase extraction and a derivatization method, which prevented the dehydration that often occurs during liquid-liquid extraction (LLE), and were identified by GC with atomic emission detection and GC-MS. As a result, cis- or trans-2-(2′-hydroxyphenyl) ethen-1-ol, previously reported as the tautomer 2-(2′-hydroxyphenyl) ethan-1-al, and 2-(2′-hydroxyphenyl) ethen-1-sulfenic acid and its isomer, 1-(1′-hydroxyethenyl) benzene-2-sulfenic acid, were identified. Benzothiophene desulfurization in culture broth at neutral pH by the strain T09 was modified as a branched metabolism from benzothiophene S-oxide based on the above compounds.
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Onaka, T., Matsui, T. & Maruhashi, K. Metabolite detection in Rhodococcus benzothiophene desulfurization using reversed-phase solid-phase extraction and a derivatization technique. Biotechnology Letters 24, 1961–1966 (2002). https://doi.org/10.1023/A:1021154528924
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DOI: https://doi.org/10.1023/A:1021154528924