Abstract
In situ hybridization using oligonucleotide probes was used to study the effects of intrastriatal microinjection of corticoliberin on the expression of the early genes c-fos, jun B, c-jun, and NGFIA in the rat brain. Administration of corticoliberin (0.25 μg) into the neostriatum induced the expression of mRNA encoded by the early genes c-fos, jun B, and NGFIA in both the neostriatum itself and in its efferent structures, particularly the nucleus accumbens and various parts of the cortex. Intrastriatal microinjection of corticoliberin had no effect on the expression of mRNA for the oncogene c-jun in the brain. These results suggest that neuronal activation in the neostriatum and its projection targets manifest as the expression of early genes is one of the mechanisms underlying the adaptive effects of corticoliberin in stress.
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REFERENCES
K. V. Anokhin, A. É. Ryabina, and K. I. Sudakov, “Expression of the c-fos gene in the mouse brain during the acquisition of defensive behavior habits,” Zh. Vyssh. Nerv. Deyat., 50, No. 1, 88–93 (2000).
K. V. Anokhin and K. V. Sudakov, “The systems organization of behavior: novelty as a learning factor in the expression of early genes in the brain during learning,” Usp. Fiziol. Nauk., 24, No. 3, 1–53 (1993).
A. I. Gorbachevskaya and O. G. Chivileva, “The organization of the afferent connections of the striatum - the structural basis for their functional organization,” Ros. Fiziol. Zh. im. I. M. Sechenova, 80, No. 1, 80–93 (1994).
E. A. Rybnikova, V. V. Rakitskaya, and V. G. Shalyapina, “Involvement of the striatum in the central regulation of the hormonal functions of the gonads,” Ros. Fiziol. Zh. im. I. M. Sechenova, 85, No. 4, 594–597 (1999).
M. O. Samoilov, The Brain and Adaptation [in Russian], St. Petersburg (1999).
E. V. Turkina, E. A. Rybnikova, V. V. Rakitskaya, and V. G. Shalyapina, “Involvement of the neostriatum in mediating the action of corticoliberin on the hormonal function of the adrenal glands,” Ros. Fiziol. Zh. im. I. M. Sechenova, 82, No. 5-6, 109–112 (1996).
E. V. Turkina, E. A. Rybnikova, V. V. Rakitskaya, and V. G. Shalyapina, “Changes in the behavioral components of stress by intrastriate administration of corticoliberin,” Ros. Fiziol. Zh. im. I. M. Sechenova, 83, No. 1-2, 150–154 (1997).
V. G. Shalyapina, E. A. Rybnikova, V. V. Rakitskaya, and E. V. Turkina, “Dopaminergic mechanisms of the neostriatum in the corticotropin-mediated regulation of adaptive behavior,” Ros. Fiziol. Zh. im. I. M. Sechenova, 84, No. 10, 1146–1151 (1998).
A. F. Yakimovskii, “The role of GABA and the glutamatergic system of the caudate nucleus of dogs in the regulation of differential inhibition,” in: The Striate System and Behavior in Normal and Pathological Conditions [in Russian], Nauka, Leningrad (1988), pp. 144–147.
H. Bading, D. D. Ginty, and M. E. Greenberg, “Regulation of gene expression in hippocampal neurons by distinct calcium signalling pathways,” Science, 260, 181–186 (1993).
A. J. Cole, R. V. Bhat, C. Patt, P. F. Worley, and J. M. Baraban, “D1 dopamine receptor activation of multiple transcription factor genes in rat striatum,” J. Neurochem., 58, No. 4, 1420–1426 (1992).
C. R. Gerfen, “The neostriatal mosaic: multiple levels of compartmental organization in the basal ganglia,” Ann. Rev. Neurosci., 15, 285–320 (1992).
C. R. Gerfen, K. A. Keefe, and E. B. Gauda, “D1-and D2-dopamine receptor function in the striatum: coactivation of D1-and D2-dopamine receptors on separate populations of neurons results in potentiated immediate early gene response in D1-containing neurons,” J. Neurosci., 15, No. 12, 8167–8176 (1995).
C. R. Gerfen, K. A. Keefe, and H. Steiner, “Dopamine-mediated gene regulation in the striatum,” Adv. Pharmacol., 25, 294–303 (1994).
K. S. Khaude, K. M. Nunley, and M. R. Bollnow, “Induction of c-fos mRNA in the brain and anterior pituitary gland by a novel environment,” Neuroreport, 4, 1079–1085 (1993).
D. J. Herrera and H. A. Robertson, “Activation of c-fos in the brain,” Progr. Neurol., 50, No. 2-3, 83–107 (1996).
P. Hughes and M. Dragunow, “Induction of immediate early genes and the control of neurotransmitter-regulated gene expression within the nervous system,” Pharmacol. Rev., 47, No. 1, 133–178 (1995).
L. Kaczmarek and A. Chandhari, “Sensory regulation of immediate-early gene expression in mammalian visual cortex: implications for functional mapping and neural plasticity,” Brain Res. Rev., 23, No. 3, 237–256 (1997).
G. F. Koob and F. E. Bloom, “Corticotropin-releasing factor and behavior,” Fed. Proc., 44, No. 1, Part 2, 259–263.
J. Kononen and M. Pelto-Huikko, “Recycling of tissue section with a sample and sensitive mRNA in situ hybridization technique TIGS,” Technical Tips Online, http/tto.trends.com/ (1997).
J. Milbrandt, “Nerve growth factor rapidly induces c-fos mRNA in PC12 rat pheocromocytoma cells,” Proc. Natl. Acad. Sci. USA, 83, 4789–4793 (1986).
J. P. Morgan, D. R. Cohen, J. L. Hemstead, and T. Curran, “Mapping patterns of c-fos expression in the central nervous system after seizure,” Science, 237, No. 4811, 192–197 (1987).
G. Paxinos and C. Watson, The Rat Brain in Stereotaxic Coordinates, Academic Press, Sydney (1986).
M. Pelto-Huikko, A. Dagerlind, J. Kononen, J. M. Lundberg, M. Villar, J. Koistinaho, R. Bravo, and T. Hokfelt, “Neuronal regulation of c-fos, c-jun, and jun B immediate-early genes in rat adrenal medulla,” J. Neurosci., 15, No. 3, Part 1, 1854–1868.
M. Sheng and M. E. Greenberg, “The regulation and function of c-fos and other immediate early genes in the nervous system,” Neuron, 4, 477–485 (1990).
D. J. Sirinathsinghji and R. P. Heavens, “Stimulation of GABA release from the rat neostriatum and globus pallidus in vivo by corticotropinreleasing factor,” Neurosci. Lett., 100, No. 1-3, 203–209 (1989).
D. J. Sirinathsinghji, F. E. Nikolarakis, and A. Herz, “Corticotropinreleasing factor stimulates the release of methionine-enkephalin and dynorphin from the neostriatum and globus pallidus of the rat: in vitro and in vivo studies,” Brain Res., 490, No. 2, 276–291 (1989).
D. J. Sirinathsinghji, K. E. Nikolarakis, S. Reimer, and A. Herz, “Nigrostriatal dopamine mediates the stimulatory effects of corticotropin releasing factor on methionine-enkephalin and dynorphin release from the rat neostriatum,” Brain Res., 526, No. 1, 172–176 (1990).
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Rybnikova, E.A., Pelto-Huikko, M. & Shalyapina, V.G. Expression of Early Genes in the Rat Brain after Administration of Corticoliberin into the Neostriatum. Neurosci Behav Physiol 33, 81–84 (2003). https://doi.org/10.1023/A:1021135516562
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DOI: https://doi.org/10.1023/A:1021135516562