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Degradation of 2,4,6-trinitrotoluene by Klebsiella sp. isolated from activated sludge

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Abstract

Klebsiella sp. strain C1 isolated from activated sludge metabolized 2,4,6-trinitrotoluene (TNT) by two different pathways. The typical metabolites in the nitro group reduction pathway of TNT, such as hydroxylamino-dinitrotoluenes and amino-dinitrotoluenes, were detected. Dinitrotoluenes and nitrite were also detected, possibly produced by a denitration pathway. After incubation of [U-14C]TNT for 28 and 77 d, 2.4 and 6.24%, respectively, were released as 14CO2. This mineralization rate was higher than those reported by any other TNT degrading bacteria and might be due to the dual pathways of degradation in this bacterium.

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Authors and Affiliations

  1. Division of Biological Sciences, Kangwon National, University, Chuncheon, 200-701, Korea

    Hyoun-Young Kim & Hong-Gyu Song

  2. Department of Biochemistry and Cell Biology, Rice, University, Houston, TX, 77005-1892, USA

    George N. Bennett

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  1. Hyoun-Young Kim
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  2. George N. Bennett
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  3. Hong-Gyu Song
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Kim, HY., Bennett, G.N. & Song, HG. Degradation of 2,4,6-trinitrotoluene by Klebsiella sp. isolated from activated sludge. Biotechnology Letters 24, 2023–2028 (2002). https://doi.org/10.1023/A:1021127201608

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