Abstract
The modulation of intracellular GABA activated 36Cl− in→out permeation across single Deiters' neuron membranes has been studied in a microchamber system. Addition of Mg2+/ATP on the membrane cytoplasmic side reduces strongly the GABA effect as does ATP alone. However, the greatest inhibition of the GABA effect is given by the addition of Mg2+ to the intracellular side buffer: a complete block of the stimulation by GABA of 36Cl− in→out permeation. This is interpreted as due to the presence in this case of a constant concentration of exogenous Mg2+ acting together with endogenous ATP in the small cytoplasmic layer on the membrane inner side. The addition of ADP to Mg2+/ATP increases the inhibitory effect of the latter. This is presumably due to an extra increase of ATP, locally under the membrane, due to phosphorylation of ADP by endogenous phosphocreatine. Overall, the data confirm that phosphorylating conditions impair the intracellular GABA action on 36Cl− in→out permeation.
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REFERENCES
Grillner, S., Hongo, T., and Lund, S. 1970. The vestibulo-spinal tract. Effect on alpha-motoneurons in the lumbo-sacral spinal cord in the cat. Exp. Brain Res. 10:94–120.
Grillner, S., and Hongo, T. 1972. Vestibulospinal effects on motoneurons and interneurons in the lumbosacral cord. Pages 243–262, in Brodal, A., and Pompeiano, O. (eds.), Progress in Brain Research. Basic Aspects of Central Vestibular Mechanisms, Elsevier, Amsterdam.
Hydén, H. 1967. The neuron, Elsevier, Amsterdam.
Ito, M., and Yoshida, M. 1964. The cerebellar evoked monosynaptic inhibition of Deiters' neurons. Experientia 20:515–516.
Obata, K., Ito, M., Ochi, R., and Sato, N. 1967. Pharmacological properties of the postsynaptic inhibition by Purkinje cell axons and the action of GABA on Deiters' neurons. Exp. Brain Res. 4:43–57.
Akaike, T., Fanardjian V. V., Ito, M., and Nakajina, H. 1973. Cerebellar control of the vestibulospinal tract cells in rabbit. Exp. Brain Res. 18:446–463.
Ito, M., Kawai, N., Udo, M., and Sato, N. 1968. Cerebellar-evoked disinhibition in dorsal Deiters' neurons. Exp. Brain Res. 6:247–264.
Okada, Y., and Shimada, C. 1976. GABA concentration in a single neuron-localization of GABA in Deiters' neuron. Brain Res. 107:658–662.
Hydén, H. 1959. Quantitative assay of compounds in isolated fresh nerve cells from control and stimulated animals. Nature 184:433–435.
Hydén, H., Lange, P. W., and Larsson, S. 1980. S-100 glia regulation of GABA transport across the nerve cell membrane. J. Neurol. Sci. 45:303–316.
Hydén, H., Cupello, A., and Palm, A. 1986. Gamma-amino butyric acid stimulates chloride permeability across microdissected Deiters' neuronal membranes. Brain Res. 379:167–170.
Rapallino, M. V., Cupello, A., and Hydén, H. 1988. Direct evidence for the presence of GABA receptors on the cytoplasmic side of the Deiters' neuron membrane. Brain Res. 462:350–353.
Cupello, A., Palm, A., Rapallino, M. V., and Hydén, H. 1991. Can Cl− ions be extruded from a γ-amino butyric acid (GABA)-acceptive nerve cell via GABAA receptors on the plasma membrane cytoplasmic side? Cell. Molec. Neurobiol. 11:333–346.
Rapallino, M. V., Cupello, A., and Hydén, H. 1993. The increase in Cl− permeation across the Deiters' neuron membrane by GABA on its cytoplasmic side is abolished by PKC activators. Cell. Molec. Neurobiol. 13:547–558.
Lauger, P. 1979. A channel mechanism for electrogenic ion pumps. Biochim. Biophys. Acta 552:143–161.
Lauger, P. (Ed). 1991. Electrogenic ionic pumps, Sinauer, Sunderland (Ma), Chapter 2.
Hydén, H., Cupello, A., and Rapallino, M. V. 1999. Chloride permeation across the Deiters' neuron plasma membrane. Activation by GABA on the membrane cytoplasmic side. Neuroscience 89:1391–1400.
Kawamoto, S., and Hidaka, H. 1984. 1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H-7) is a selective inhibitor of protein kinase C in rabbit platelets. Biochem. Biophys. Res. Comm. 125:258–264.
Garland, L. G., Bonser, R. W., and Thompson, N. T. 1987. Protein kinase C inhibitors are not selective. Trends Pharmacol. Sci. 8:334.
Cummins, J., and Hydén, H. 1962. Adenosine triphosphate levels and adenosine triphosphatases in neurons, glia and neuronal membranes of the vestibular nucleus. Biochem. Biophys. Acta 60:271–283.
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Rapallino, M.V., Cupello, A. & Hydén, H. Intracellular GABA-Activated In→Out Permeation of Chloride Across the Deiters' Neuron Membrane: Modulation by Phosphorylating Activities. Neurochem Res 24, 1089–1092 (1999). https://doi.org/10.1023/A:1021073230464
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DOI: https://doi.org/10.1023/A:1021073230464