Abstract
Chimeric constructs were synthesized based on oligoribonucleotides modified at the 2"-position of the ribose (2"-O-tetrahydropyranyl- or 2"-O-methyl-) and at the 3"-terminus of the oligonucleotide chain (terminal 3"—3" internucleotide linkage), which are complementary to a region of MDR 1 mRNA. A comparative study of the properties of these chimeric constructs was performed. The chimeric oligomers with the modified 3"-terminus are characterized by high stability with respect to 3"-exonucleases, form stable complementary complexes with RNA, and can activate RNase H in a duplex with RNA.
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Novopashina, D.S., Kuznetsova, M.A., Komarova, N.I. et al. Chimeric oligonucleotides based on 2"-O-modified oligoribonucleotides with the terminal 3"—3" internucleotide linkage as potential inhibitors of MDR 1 gene expression. Russian Chemical Bulletin 51, 1217–1221 (2002). https://doi.org/10.1023/A:1020996328700
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DOI: https://doi.org/10.1023/A:1020996328700