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Directed cleavage of RNA within an imperfect complementary complex by oligonucleotide—bleomycin A5 conjugates

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Abstract

It was demonstarted for the first time that RNA can be subjected to site-specific oxidative cleavage induced by the glycopeptide antibiotic bleomycin A5 (Blm) covalently linked to the 3"-terminus of an oligodeoxyribonucleotide through two, three, or four residues of hexaethylene glycol phosphate (p-heg) n . The oligonucleotide conjugate with bleomycin forms an imperfect complementary complex with the RNA to be cleaved (5"-prCGGAGUUGGAAAACAAUGAAAAGGCCCCCA/Blm-(p-heg) n -3"-pdGCCTCACCTTTTGTTA). The cleavage occurs at the only nucleotide residue (U) preceding a one-nucleotide bulge in the RNA chain, which is formed due to imperfect complementarity between the oligodeoxyribonucleotide and the RNA to be cleaved.

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Authors and Affiliations

  1. Siberian Branch of the Russian Academy of Sciences, Novosibirsk Institute of Bioorganic Chemistry, 8 prosp. Akad. Lavrent"eva, 630090, Novosibirsk, Russian Federation

    P. E. Vorobjev, I. A. Pyshnaya & V. F. Zarytova

  2. Thomas Jefferson University, USA, Philadelphia, PA, 19107, Walnut Street 1025, Suite 425

    E. Wickstrom

Authors
  1. P. E. Vorobjev
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  2. I. A. Pyshnaya
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  3. E. Wickstrom
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  4. V. F. Zarytova
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Vorobjev, P.E., Pyshnaya, I.A., Wickstrom, E. et al. Directed cleavage of RNA within an imperfect complementary complex by oligonucleotide—bleomycin A5 conjugates. Russian Chemical Bulletin 51, 1187–1189 (2002). https://doi.org/10.1023/A:1020984025975

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  • DOI: https://doi.org/10.1023/A:1020984025975

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