Abstract
Using Agrobacterium rhizogenes, Astragalus sinicus plants were transformed with the kidney bean pal5 gene coding for phenylalanine-ammonia lyase (PAL). The hairy root culture thus obtained manifested enhanced PAL activity and lignin content in the cell walls; in addition, the transformed cells differed from the wild-type ones in several electrophysiological indices. In particular, the diffusion component of the total membrane potential of plasmalemma increased in the pal-transformed roots. The authors presume that the volume density of the protein-related negative charge of the cytoplasm increases in the transformed root cells along with changes in the cytoplasmic pH and pCa2+, the extent of coupling of these two indices, and the hydraulic conductivity of plasmodesmata.
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REFERENCES
Cho, H.J., Widholm, J.M., Tanaka, N., Nakanishi, Y., and Murooka, Y., Agrobacterium rhizogenes-Mediated Transformation and Regeneration of the Legume Astragalus sinicus (Chinese Milk Vetch), Plant Sci., 1998, vol. 138, pp. 53-65.
Mano, Y., Variation among Hairy Root Clones and Its Application, Plant Tissue Cult. Lett., 1989, vol. 6, pp. 1-9.
Tepfer, D., Genetic Transformation Using Agrobacterium rhizogenes, Physiol. Plant., 1990, vol. 79, pp. 140-146.
Tolvonen, L., Utilization of Hairy Root Cultures for Production of Secondary Metabolites, Biotechnol. Prog., 1993, vol. 9, pp. 12-20.
Tarchevsky, I.A., Maksyutova, N.N., Yakovleva, V.G., and Grechkin, A.N., Succinic Acid Is a Mimetic of Salicylic Acid, Fiziol. Rast. (Moscow), 1999, vol. 46, pp. 23-28 (Russ. J. Plant Physiol., Engl. Transl.).
Edwards, K., Gramer, C.L., Bodwell, G.P., Dixon, R.A., Schuch, W., and Lamb, C.J., Rapid Transient Induction of Phenylalanine-Ammonia Lyase mRNA in Elicitor-Treated Bean Cells, Proc. Natl. Acad. Sci. USA, 1985, vol. 82, pp. 6731-6735.
Maniatis, T., Sambrook, J., and Fritsh, E.E., Molecular Cloning: A Laboratory Manual, Nolan, C.N.Y., Ed., Cold Spring Harbor: Cold Spring Harbor Lab., 1989.
Bradford, M.M., A Rapid and Sensitive Method of the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding, Anal. Biochem., 1976, vol. 72, pp. 248-254.
Bruce, P.J. and West, C.A., Elicitation of Lignin Biosynthesis and Isoperoxidase Activity by Pectic Fragments in Suspension Culture of Castor Bean, Plant Physiol., 1989, vol. 91, pp. 889-897.
Velikanov, G.A., Tsentsevitskii, A.N., Nuriev, I.Kh., Evarestov, A.S., and Gordon, L.Kh., Cell-to-Cell Transfer of Water and Ions in Wheat Seedling Roots, Fiziol. Rast. (Moscow), 1992, vol. 39, pp. 972-982 (Sov. Plant Physiol., Engl. Transl.).
Fedorov, G.E., Il'yushenko, A.S., Korolev, N.P., Mil'gram, V.D., Gus'kova, R.A., Kondakov, E.V., Osipov, V.I., and Ivanov, I.I., The Effect of Local Anesthetics on the Electrical Properties of Plate Black Bilayers, Izv. Akad. Nauk SSSR, Ser. Biol., 1990, no. 3, pp. 353-358.
Piruzian, E.S., Osnovy geneticheskoi inzhenerii (Basics of Plant Genetic Engineering), Moscow: Nauka, 1988.
Sewalt, V.J.H., Ni, W., Blount, J.W., Jung, H.G., Masoud, S.A., Howles, P.A., Lamb, C., and Dixon, R.A., Reduced Lignin Content and Altered Lignin Composition in Transgenic Tobacco Down-Regulated in Expression of L-Phenylalanine-Ammonia Lyase or Cinnamate 4-Hydroxylase, Plant Physiol., 1997, vol. 115, pp. 41-50.
Velikanov, G.A., Volobueva, O.V., and Khokhlova, L.P., The Study of the Hydraulic Conductivity of the Plasmodesmal Transport Channels by the Pulse NMR Method, Fiziol. Rast. (Moscow), 2001, vol. 48, pp. 375-383 (Russ. J. Plant Physiol., Engl. Transl.).
Kauss, H., Some Aspects of Calcium-Dependent Regulation in Plant Metabolism, Annu. Rev. Plant Physiol., 1987, vol. 38, pp. 47-72.
Lyalin, O.O., Ktitorova, I.N., Barmicheva, E.M., and Akhmedov, N.I., Intercellular Contacts of Submersed Trichomes of Salvinia, Fiziol. Rast. (Moscow), 1986, vol. 33, pp. 432-446 (Sov. Plant Physiol., Engl. Transl.).
Holdaway-Clarke, T.L., Walker, N.A., Hepler, P.K., and Overal, R.L., Physiological Elevations in Cytoplasmic Free Calcium by Cold or Ion Injection Result in Transient Closure of Higher Plant Plasmodesmata, Planta, 2000, vol. 210, pp. 329-335.
Perlin, D.S. and Spenswick, R.M., Characterization of ATPase Activity Associated with Corn Leaf Plasma Membrane, Plant Physiol., 1981, vol. 68, pp. 521-526.
Ketchum, K.A., Shrier, A., and Poole, R.J., Characterization of Potassium-Dependent Currents in Protoplasts of Corn Suspension Cells, Plant Physiol., 1989, vol. 89, pp. 1184-1192.
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Krasnyanskaya, V., Belova, L.P., Velikanov, G.A. et al. Expression of the Kidney Bean Phenylalanine-Ammonia Lyase Gene in the Hairy Roots of Astragalus sinicus. Russian Journal of Plant Physiology 49, 804–809 (2002). https://doi.org/10.1023/A:1020973831060
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DOI: https://doi.org/10.1023/A:1020973831060