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Sequence-specific RNA cleavage by oligonucleotide-peptide conjugates

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Abstract

This paper considers the site-directed cleavage of the in vitro transcript of human tRNALys 3 by the conjugate CNH2Gly(ArgLeu)3ArgLeuN—p-DAG—5"CCCTGGACCCTCAGAT3" (conjugate pep-1A) of the oligodeoxyribonucleotide 1A (CCCTGGACCCTCAGAT) with a peptide [LeuArg]4Gly attached at the 5" terminus of the oligonucleotide through diethylene glycol as a linker. Under physiological conditions, the conjugate pep-1A cleaved the C56-A57 phosphodiester bond of the RNA target with high efficacy. In addition, the conjugate cleaved several other bonds in the D loop, at the basis of the amino-acceptor stem, and in the anticodon hairpin due, probably, to the formation of imperfect complexes of the conjugate with partially complementary sequences in the RNA structure.

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Authors and Affiliations

  1. Siberian Branch of the Russian Academy of Sciences, Novosibirsk Institute of Bioorganic Chemistry, 8 prosp. Akad. Lavrent"eva, 630090, Novosibirsk, Russian Federation

    N. L. Mironova, D. V. Pyshnyi, E. M. Ivanova, V. F. Zarytova, M. A. Zenkova & V. V. Vlassov

  2. Novosibirsk State University, 2 ul. Pirogova, 630090, Novosibirsk, Russian Federation

    N. L. Mironova & V. V. Vlassov

  3. Institute of Biochemistry, Biocenter, D-97074, Wurzburg, Germany

    H. J. Gross

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  1. N. L. Mironova
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  2. D. V. Pyshnyi
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  3. E. M. Ivanova
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  4. V. F. Zarytova
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  5. M. A. Zenkova
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  6. H. J. Gross
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  7. V. V. Vlassov
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Mironova, N.L., Pyshnyi, D.V., Ivanova, E.M. et al. Sequence-specific RNA cleavage by oligonucleotide-peptide conjugates. Russian Chemical Bulletin 51, 1177–1186 (2002). https://doi.org/10.1023/A:1020932009136

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