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Effects of Neonatal Cerebral Hypoxia-Ischemia on the In Vitro Phosphorylation of Synapsin 1 in Rat Synaptosomes

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Abstract

Synapsins are phosphoproteins related to the anchorage of synaptic vesicles to the actin skeleton. Hypoxia-ischemia causes an increased calcium influx into neurons through ionic channels gated by activation of glutamate receptors. In this work seven-day-old Wistar rats were submitted to hypoxia-ischemia and sacrificed after 21 hours, 7, 30, or 90 days. Synaptosomal fractions were obtained by Percoll gradients and incubated with 32P (10μCi/g). Proteins were analysed by SDS-PAGE and radioactivity incorporated into synapsin 1 was counted by liquid scintillation. Twenty-one hours after hypoxia-ischemia we observed a reduction on the in vitro phosphorylation of synapsin 1, mainly due to hypoxia, rather than to ischemia; this effect was reversed at day 7 after the insult. There was another decrease in phosphorylation 30 days after the event interpreted as a late effect of hypoxia-ischemia. No changes were observed at day 90. Our results suggest that decreased phosphorylation of synapsin 1 could be related to neuronal death that follows hypoxia-ischemia.

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Authors and Affiliations

  1. Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos 2600 - anexo, 90035-003, Porto Alegre- RS -, Brasil

    Maria Beatriz Moretto, Ângela de Mattos-Dutra, Nice Arteni, Renata Meirelles, Marta Sampaio de Freitas, Carlos Alexandre Netto & Regina Pessoa-Pureur

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  1. Maria Beatriz Moretto
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  2. Ângela de Mattos-Dutra
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  3. Nice Arteni
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Moretto, M.B., de Mattos-Dutra, Â., Arteni, N. et al. Effects of Neonatal Cerebral Hypoxia-Ischemia on the In Vitro Phosphorylation of Synapsin 1 in Rat Synaptosomes. Neurochem Res 24, 1263–1269 (1999). https://doi.org/10.1023/A:1020925107130

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