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The construction of a linkage map of Alstroemeria aurea by AFLP markers

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Abstract

An AFLP based linkage map has been generated for the ornamental crop species Alstroemeria aurea. In view of the large genome size of Alstroemeria (25,000 Mb) the number of selective nucleotides for AFLP amplification was increased to EcoRI+4/MseI+4 to generate fingerprints of moderate complexity. In addition, markers were generated with the enzyme combination Sse/Mse, where Sse8387I is an8-cutter, thereby reducing AFLP template complexity. Segregation of 374AFLP polymorphisms was recorded in the F1 of an intraspecific A. aurea cross (A002 × A003). The map consisted of 8 A002 and 10A003 linkage groups with 122 and 214 markers covering 306.3 and605.6 cM, respectively. The two maps were integrated by using the21% of the AFLP markers that were heterozygous in both parents, and31% of the markers remained unlinked. Pollen color was assigned to linkage group A002-6. The enzyme combinations EcoRI+4/MseI+4 and Sse+2/MseI+3 generated 80 and 30 clear bands per lane with 16 and 9 polymorphic markers, respectively. Twenty percent of the EcoRI+4/MseI+4 primer combinations resulted in fingerprints that were disturbed by a few excessively thick bands (55 out of 288 primer combinations). We conclude that fingerprints and markers generated with the eight-cutter enzyme Sse8387I, in combination with+2/+3 selective nucleotides (Sse+2/MseI+3) are superior toEcoRI+4/MseI+4.

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References

  • Aker, S. & W. Healy, 1990. The phytogeography of the genus Alstroemeria. Herbertia 46: 76–87.

    Google Scholar 

  • Anastassopoulos, E. & M. Keil, 1996. Assessment of natural and induced genetic variation in Alstroemeria using random amplified polymorphic DNA (RAPD) markers. Euphytica 90: 235–244.

    Article  CAS  Google Scholar 

  • Bennett, M.D., A.V. Cox, & I.J. Leitch, 1998. Angiosperm DNA Cvalues database. http://www.rbgkew.org.uk/cval/database1.html.

  • Buitendijk, J.H., 1998. A cytological characterization of genomes of Alstroemeria, the production of interspecific hybrids, and their performance during micropropagation. Thesis Department of Plant Breeding, Wageningen, The Netherlands.

    Google Scholar 

  • Buntjer, J.B., 1999. Cross Checker Fingerprint analysis software v2.9, Wageningen University and Research Centre, The Netherlands.

    Google Scholar 

  • Bayer, E., 1987. Die Gattung Alstroemeria in Chile. Mitt. Bot. Staatssamml. München 24: 1–362.

    Google Scholar 

  • de Jeu, M.J., J. Lasschuit, A.G.J. Kuipers, S.A. Kamstra & R.G.F. Visser, 1997. Characterization and localization of repetitive DNA sequences in the ornamental Alstroemeria aurea Graham. Theor Appl Genet 94: 982–990.

    Article  CAS  Google Scholar 

  • Dubouzet, J.G., N. Murata & K. Shinoda, 1997. RAPD analysis of genetic relationships among Alstroemeria L. cultivars. Sci Hortic 68: 181–189.

    Article  CAS  Google Scholar 

  • Grattapaglia, D. & R. Sederoff, 1994. Genetic linkage maps of Eucalyptus grandis and E. urophylla using a pseudo-testcross: mapping strategy and RAPD markers. Genetics 137: 1121–1137.

    PubMed  CAS  Google Scholar 

  • Han, T.H., H.J. van Eck, M.J. de Jeu & E. Jacobsen, 1999. Optimization of AFLP fingerprinting of organisms with a large genome size: A study on Alstroemeria spp. Theor Appl Genet 98: 465–471.

    Article  Google Scholar 

  • Han, T.H., M.J. de Jeu, H. van Eck & E. Jacobsen, 2000. Genetic diversity of Chilean and Brazilian Alstroemeria species assessed by AFLP analysis. Heredity 84: 564–569.

    Article  PubMed  Google Scholar 

  • Hemmat, M., N.F. Weeden, A.G. Manganaris & D.M. Lawson, 1994. Molecular marker linkage map for apple. J Hered 85: 4–11.

    PubMed  CAS  Google Scholar 

  • Kamstra, S.A., A.G.J. Kuipers, M.J. de Jeu, M.S. Ramanna & E. Jacobsen, 1999. The extent and position of homoeologous recombination in a distant hybrid of Alstroemeria: a molecular cytogenetic assessment of first generation backcross progenies. Chromosoma 108: 52–63.

    Article  PubMed  CAS  Google Scholar 

  • Karlin, S. & J. Mrázek, 1997. Compositional differences within and between eukaryotic genomes. Proc Natl Acad Sci USA 94: 10227–10232.

    Article  PubMed  CAS  Google Scholar 

  • Lespinasse, D., M. Rodier-Goud, L. Grivet, A. Leconte, H. Legnate & M. Seguin, 2000. A saturated genetic linkage map of rubber tree (Hevea spp.) based on RFLP, AFLP, microsatellite, and isozyme markers. Theor Appl Genet 100: 127—138.

    Google Scholar 

  • Maliepaard, C., J. Jansen & J.W. van Ooijen, 1997. Linkage analysis in a full-sib family of an outbreeding plant species: overview and consequences for applications. Genet Res 70: 237–250.

    Article  Google Scholar 

  • Maliepaard, C., F.H. Alston, G. Van Arkel, L.M. Brown, E. Chevreau, F. Dunemann, K.M. Evans, S. Gardiner, P. Guilford, A.W. van Heusden, J. Janse, F. Laurens, J.R. Lynn, A.G. Manganaris, A.P.M. den Nijs, N. Periam, E. Rikkerink, P. Roche, C. Ryder, S. Sansavini, H. Schmidt, S. Tartarini, J.J. Verhaegh, M. Vrielinkvan Ginkel & G.J. King, 1998. Aligning male and female linkage maps of apple (Malus pumila Mill.) using multi-allelic markers. Theor Appl Genet 97: 60–73.

    Article  CAS  Google Scholar 

  • Picton, D.D. & H.G. Hughes, 1997. Characterization of Alstroemeria species using Random Amplified Polymorphic DNA (RAPD) analysis. Hort Science 32: 482, Abstract: 323.

    Google Scholar 

  • Qi, X. & P. Lindhout, 1997. Development of AFLP markers in barley. Mol Gen Genet 254: 330–336.

    Article  PubMed  CAS  Google Scholar 

  • Rogers, S.O. & A.J. Bendich, 1988. Extraction of DNA from plant tissues. Plant Mol Bio Manual 6: 1–10.

    Google Scholar 

  • Rouppe van der Voort, J.N.A.M., P. van Zandvoort, H.J. van Eck, R.T. Folkertsma, R.C.B. Hutten, J. Draaistra, F.J. Gommers, E. Jacobsen, J. Helder & J. Bakker, 1997. Use of allele specificity of comigrating AFLP markers to align genetic maps from different potato genotypes. Mol Gen Genet 255: 438–447.

    Article  PubMed  CAS  Google Scholar 

  • Stam, P., 1993. Construction of integrated genetic linkage maps by means of a new computer package: JoinMap. Plant J 3: 739–744.

    Article  CAS  Google Scholar 

  • Stam, P. & J.W. Van Ooijen, 1995. JoinMapTM version 2.0: software for the calculation of genetic linkage maps. CPRO-DLO, Wageningen.

    Google Scholar 

  • Tsuchiya, T., A. Hang, W.E. Healy & H. Hughes, 1987. Chromosome studies in the genus Alstroemeria. I. Chromosome numbers in 10 cultivars. Bor Gaz 148: 519–524.

    Article  Google Scholar 

  • Uphof, J.C.T., 1952. A review of the genus Alstroemeria. Plant Life 8: 37–53.

    Google Scholar 

  • Van Berloo, R., 1999. Computer note. GGT: software for the display of graphical genotypes. J Hered 90: 328–329.

    Article  Google Scholar 

  • Van Eck, H.J., J.R. van der Voort, J. Draaistra, P. van Zandvoort, E. van Enckevort, B. Segers, J. Peleman, E. Jacobsen, J. Helder & J. Bakker, 1995. The inheritance and chromosomal localization of AFLP markers in a non-inbred potato offspring. Mol Breeding 1: 397–410.

    Article  CAS  Google Scholar 

  • Van Heusden, A.W., J.W. van Ooijen, R. Vrielink-van Ginkel, W.H.J. Verbeek, W.A. Wietsma & C. Kik, 2000. A genetic map of an interspecific cross in Allium based on amplified fragment length polymorphism (AFLPTM) markers. Theor Appl Genet 100: 118–126.

    Article  CAS  Google Scholar 

  • Van Os, H., J. Buntjer, P. Stam & H.J. van Eck, 2000. Evaluation of Two Algorithms for the Construction of Genetic Linkage Maps. Poster. Plant & Animal Genome VIII Conference, San Diego, CA, January 9- 12, 2000.

  • Vos, P., R. Hogers, M. Bleeker, M. Reijans, T. van de Lee, M. Hornes. A. Frijters, J. Pot, J. Peleman, M. Kuiper & M. Zabeau, 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23: 4407–4414.

    PubMed  CAS  Google Scholar 

  • Waugh, R., N. Bonar, E. Baird, B. Thomas, A. Graner, P. Hayes & W. Powell, 1997. Homology of AFLP products in three mapping populations of barley. Mol Gen Genet 255: 311–321.

    Article  PubMed  CAS  Google Scholar 

  • Zabeau, M. & P. Vos, 1993. Selective restriction fragment amplification: a general method for DNA fingerprinting. European Patent Application 92402629.7; Publ no. EP 0534858 A1.

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  1. Laboratory of Plant Breeding, The Graduate School Experimental Plant Sciences Wageningen University, P.O. Box 386, NL-6700 AJ, Wageningen, The Netherlands

    Tae-Ho Han, Herman van Eck, Marjo de Jeu & Evert Jacobsen

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  1. Tae-Ho Han
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  2. Herman van Eck
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  3. Marjo de Jeu
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  4. Evert Jacobsen
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Han, TH., van Eck, H., de Jeu, M. et al. The construction of a linkage map of Alstroemeria aurea by AFLP markers. Euphytica 128, 153–164 (2002). https://doi.org/10.1023/A:1020921103374

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