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Enhancement of β-glucosidase stability and cellobiose-usage using surface-engineered recombinant Saccharomyces cerevisiae in ethanol production

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Abstract

To enhance the use of cellobiose by a recombinant Sachharomyces cerevisiae, the expressed β-glucosidase that hydrolyzes cellobiose was stabilized using a surface-display system. The C-terminal half of α-agglutinin was used as surface-display motif for the expression of β-glucosidase in the cell wall. The surface-displayed β-glucosidase had a half-life time (t 1/2) of 100 h in acidic culture broth conditions, while secreted β-glucosidase had a t 1/2 of 60 h. With such stabilization of β-glucosidase, the surface-engineered S. cerevisiae utilized 7.5 g cellobiose l−1 over 60 h, while S. cerevisiae secreting β-glucosidase into culture broth used 5.8 g cellobiose l−1 over the same period.

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Authors and Affiliations

  1. School of Chemical Engineering, Seoul National University, Seoul, 151-742, Korea

    Seung Pil Pack & Young Je Yoo

  2. Eugene Science, Inc., Donggyo-dong, Mapo-gu, Seoul, 121-754, Korea

    Kyungmoon Park

Authors
  1. Seung Pil Pack
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  2. Kyungmoon Park
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  3. Young Je Yoo
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Pack, S.P., Park, K. & Yoo, Y.J. Enhancement of β-glucosidase stability and cellobiose-usage using surface-engineered recombinant Saccharomyces cerevisiae in ethanol production. Biotechnology Letters 24, 1919–1925 (2002). https://doi.org/10.1023/A:1020908426815

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  • DOI: https://doi.org/10.1023/A:1020908426815

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