Abstract
Behaviors with high energetic costs may decrease in frequency in domestic animals as a response to selection for increased production. The aim of this study was to quantify production traits, foraging behavior, and social motivation in F2 progeny from a White Leghorn × red junglefowl intercross (n = 751–1046) and to perform QTL analyses on the behavioral traits. A foraging-social maze was used for behavioral testing, which consisted of four identical arms and a central box. In two arms there was ad libitum access to the birds' usual food, and in the other two there was novel food (sunflower seeds) mixed with cat litter. In one arm with each of the two food sources, social stimuli were simulated by the presence of a mirror. Each bird could therefore feed on novel or well known food either alone or in the perceived company of a conspecific. Egg production, sexual maturity (females), food intake, and growth were measured individually, and residual food intake and metabolic body weight were estimated using standard methods. A genome scan using 104 microsatellite markers was carried out to identify QTLs affecting behavioral traits. Phenotypic growth rates at different ages showed weak associations in both sexes. Sexual maturity and egg weight were not strongly correlated to growth, indicating that these traits are not genetically linked. Time spent in each arm and in the central part of the maze was analyzed using principal component analyses. Four principal components (PC) were extracted, each reflecting a pattern of behavior in the maze. Females with early onset of sexual maturity scored higher on the PC1 reflecting preference for free food without social stimuli, and females with higher egg production scored higher on the PC2 reflecting exploration. Males with an overall higher growth rate and higher residual food intake scored higher on the PC3, which possibly reflected fear of the test situation, and tended to score higher on the PC4 reflecting low contrafreeloading. Significant QTLs were found for PC1 and PC4 scores on chromosomes 27 and 7, respectively. The location of the QTLs coincided with known QTLs for growth rate and body weight. The results suggest a trade-off between energy-demanding behavior and high production and that some of this may be caused by genetic linkage or pleiotropic gene effects.
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REFERENCES
Andersson, L. (2001). Genetic dissection of phenotypic diversity in farm animals. Nature Genet. 2(2):130–138.
Beilharz, R. G. (1998). Environmental limit to genetic change. An alternative theorem of natural selection. J. Anim. Breed. Genet. 115:433–437.
Beilharz, R. G., Luxford, B. G., and Wilkinson, J. L. (1993). Quantitative genetics and evolution: Is our understanding of genetics sufficient to explain evolution? J. Anim. Breed. Genet. 110(3): 161–170.
Beilharz, R. G., and Nitter, G. (1998). The missing E: the role of the environment in evolution and animal breeding. J. Anim. Breed. Genet. 115:439–453.
Braastad, B. O., and Katle, J. (1989). Behavioral differences between laying hen populations selected for high and low efficiency of food utilization. Br. Poult. Sci. 30:533–544.
Bradshaw, R. H. (1992). Concpecific discrimination and social preference in the laying hen. Appl. Anim. Behav. Sci. 33(1):69–75.
Carder, B., and Berkowitz, K. I. (1970). Rats preference for earned in comparison with free food. Science. 167:1273–1274.
Carlborg, Ö., Andersson-Eklund, L., and Andersson, L. Parallel computing in interval mapping of Quantitative Trait Loci. J. Hered., in press.
Churchill, G. A., and Doerge, R. W. (1994). Empirical threshold values for quantitative trait mapping. Genetics 138:963–971.
Deerenberg, C., and Overkamp, G. J. F. (1999). Hard work impinges on fitness: an experimental study with zebra finches. Anim. Behav. 58:173–179.
Duncan, I. J. H., and Hughes, B. O. (1972). Free and operant feeding in domestic fowls. Anim. Behav. 20:775–777.
Flint, J., Corley, R., DeFries, J. C., Fulker, D. W., Gray, J. A., Miller, S., and Collins, A. C. (1995). A simple genetic basis for a complex psychological trait in laboratory mice. Science 269:1432–1435.
Forkman, B. A. (1993). Self-reinforced behavior does not explain contra-freeloading in the Mongolian gerbil. Ethology 94:109–112.
Green, P., Falls, K., and Crook, S. (1990). Documentation for CRIMAP, version 2.4., Washington University School of Medicine, St. Louis, MO.
Guhl, A. M. (1962). The behavior of chickens. The Behavior of Domestic Animals. E. S. E. Hafez, London, Bailliére, Tindall & Cox; pp. 491–530.
Gunnarsson, S. (2000). Laying hens in loose housing systems. Clinical, ethological and epidemiological aspects. Doctoral thesis at the Swedish University of Agricultural Sciences. Uppsala, Sweden. Haley, C. S., Knott, S. A., and Elsen, J. M. (1994). Mapping quantitative trait loci in crosses between outbred lines using least squares. Genetics 136:1195–1207.
Hunt, G. J., Guzmán-Novoa, E., Fondrk, M. K., and Page, R. E. Jr. (1998). Quantitative trait loci for honey bee stinging behavior and body size. Genetics 148:1203–1213.
Inglis, I. R., and Ferguson, N. J. K. (1986). Starlings search for food rather than eat freely-available, identical food. Anim. Behav. 34:614–616.
Inglis, I. R., Forkman, B., and Lazarus, J. (1997). Free food or earned food? A review and fuzzy model of contrafreeloading. Anim. Behav. 53:1171–1191.
Liljedahl, L.-E., Kolstad, N., Soerensen, P., and Maijala, K. (1979). Scandinavian selection and crossbreeding experiment with laying hens, I. Background and general outline. Acta Agric. Scand. 29:273–285.
Luiting, P., and Urff, E. M. (1991). Residual feed consumption in laying hens. 1. Quantification of phenotypic variation and repeatabilities. Poult. Sci. 70:1655–1662.
Neuringer, A. J. (1969). Animals respond for food in the presence of free food. Science 16:399–401.
Page R. E., Jr., Fondrk, M. K., Hunt, G. J., Guzmán-Novoa, E., Humpshires, M. A., Nguyen, K., and Greene, A. S. (2000). Genetic dissection of Honeybee (Apis mellifera L.) foraging behavior. J. Hered. 91(6):474–479.
Price, E. O. (1999). Behavioral development in animals undergoing domestication. Appl. Anim. Behav. Sci. 65(3):245–271.
Ramos, A., Moisan, M.-P., Chaouloff, F., Mormède, C., and Mormède, P. (1999). Identification of female-specific QTL affecting an emotionality-related behavior in rats. Mol. Psych. 4:453–462.
Rauw, W. M., Kanis, E., Noordhuizen-Stassen, E. N., and Grommers, F. J. (1998). Undesirable side effects of selection for high production efficiency in farm animals: a review. Livest. Prod. Sci. 56:15–33.
Schmutz, S. M., Stookey, J. M., Winkelman-Sim, D. C., Waltz, C. S., Plante, Y., and Buchanan, F. C. (2001). A QTL study of cattle behavioral traits in embryo transfer families. J. Hered. 92:290–292.
Schütz, K. E., Forkman, B., and Jensen, P. (2001). Domestication effects on foraging strategy, social behavior and different fear responses: a comparison between the red junglefowl (Gallus gallus) and a modern layer breed. Appl. Anim. Behav. Sci. 74: 1–14.
Schütz, K. E., and Jensen, P. (2001). Effects of resource allocation on behavioral strategies: a comparison of red junglefowl (Gallus gallus) and two domesticated breeds of poultry. Ethology 107:753–765.
Talbot, C. J., Nicod, A., Cherny, S. S., Fulker, D. W., Collins, A. C., and Flint, J. (1999). High-resolution mapping of quantitative trait loci in outbred mice. Nat. Genet. 21:305–308.
Turri, M. G., Datta, S. R., DeFries, J., Henderson, N. D., and Flint, J. (2001a). QTL analysis identifies multiple behavioral dimensions in ethological tests of anxiety in laboratory mice. Curr. Biol. 11:725–734.
Turri, M. G., Henderson, N. D., DeFries, J. C., and Flint, J. (2001b). Quantitative trait locus mapping in laboratory mice derived from a replicated selection experiment for open-field activity. Genetics 158:1217–1226.
Wehner, J. M., Radcliff, R. A., Rossman, S. T., Christensen, S. C., Rasmussen, D. L., Fulker, D. W., and Wiles, M. (1997). Quantitative trait locus analysis of contextual fear conditioning in mice. Nat. Genet. 17:331–334.
Weller, J. I. (2001). Quantitative trait loci analysis in animals. Wallingford, CABI Publishing.
West, B., and Zhou, B.-X. (1989). Did chickens go north? New evidence for domestication. World's Poult. Sci. J. 45:205–218.
Wood-Gush, D. G. M. (1955). The behavior of the domestic chicken: a review of the literature. Br. J. Anim. Behav. 3:81–110.
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Schütz, K., Kerje, S., Carlborg, Ö. et al. QTL Analysis of a Red Junglefowl × White Leghorn Intercross Reveals Trade-Off in Resource Allocation Between Behavior and Production Traits. Behav Genet 32, 423–433 (2002). https://doi.org/10.1023/A:1020880211144
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DOI: https://doi.org/10.1023/A:1020880211144