Abstract
Linear Alkylbenzene Sulfonates (LAS) are the most widely used synthetic anionicsurfactants. They are anthropogenic, toxic compounds and are found in the primarysludge generated in municipal wastewater treatment plants. Primary sludge is usuallystabilized anaerobically and therefore it is important to investigate the effect of thesexenobiotic compounds on an anaerobic environment. The inhibitory effect of LinearAlkylbenzene Sulfonates (LAS) on the acetogenic and methanogenic step of theanaerobic digestion process was studied. LAS inhibit both acetogenesis from propionate and methanogenesis from acetate and hydrogen and it is shown that the propionate-utilising bacteria are more sensitive to the presence of LAS than the acetoclastic methanogens. It has been proven that the inhibition intensity depends on the solids concentration and thus the term ``biomass specific LAS concentration'' has been introduced in order to describe the phenomenon better. Conclusively, it is believed that the inhibitory effect of LAS is the main reason that anaerobic microbial enrichments on LAS have not been succeeded yet. Also, the inhibition caused by LAS on the acetogenic and methanogenic step of the anaerobic digestion process should be seriously taken into consideration when wastewater from a surfactant producing industry is to be treated biologically or enter a municipal wastewater treatment plant that employs anaerobic technology. The upper allowable biomass specific LAS concentration should be 14 mg LAS (gVSS)-1.
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References
Aeckersberg F, Bak F & Widdel F (1991) Anaerobic oxidation of saturated hydrocarbons to CO2 by a new type of sulfate-reducing bacterium. Arch. Microbiol. 156: 5-14
Andrews JF (1969) Dynamic model of the anaerobic digestion process. J. Sanit. Engng. Div. Am. Soc. Civ. Engrs. SA 1: 95-116
Angelidaki I & Ahring BK (1992) Effects of free long-chain fatty acids on thermophilic anaerobic digestion. Applied Microbiology and Biotechnology 37: 808-812
Angelidaki I, Mogensen AS & Ahring BK (2000) Degradation of organic contaminants found in organic waste. Biodegradation 11: 377-383
APHA (American Public Health Association, American Water Works Association, Water Pollution Control Federation) (1989) Standard methods for the examination of water and wastewater, 17th edition, Washington
Cavalli L, Cassani G & Lazzarin M (1996) Biodegradation of linear alkylbenzene sulphonate (LAS) and alcohol ethoxylate (AE). Tenside Surfact. Deterg. 33: 158-165
Cook AM (1998) Sulfonated surfactants and related compounds: facets of their desulfonation by aerobic and anaerobic bacteria. Tenside Surfact. Deterg. 35: 52-56
Federle TW & Schwab BS (1992) Mineralization of surfactants in anaerobic sediments of a laundromat wastewater pond.Wat. Res. 26: 123-127
Grbic-Galic D & Vogel TM (1987) Transformation of toluene and benzene by mixed methanogenic cultures. Appl. Envir. Microbiol. 53: 254-260
Hamilton WA (1971) Membrane active antibacterial compounds. In: WB Hugo (Ed), Inhibition and destruction of the microbial cell, Academic Press, London
Henderson C (1973) The effects of fatty acids on pure cultures of rumen bacteria. J. Agric. Sci. 81: 107-112
Hofer R, Jeney Z & Bucher F (1995) Chronic effects of Linear Alkylbenzene Sulfonate (LAS) and ammonia on rainbow trout (Oncorhynchus Mykiss) fry at water criteria limits Wat. Res. 29: 2725-2729
Ierusalimsky ND (1967) Bottle-necks in metabolism as growth rate controlling factors. In: Microbial Physiology and continuous culture, third international Symposium, EO Powell, CGT
Evans, RE Strange & DW Tempest (eds), Her Majesty's Stationery Office, London, 23-33
Lewis MA (1991) Chronic and sublethal toxicities of surfactants to aquatic animals: a review and risk assessment. Wat. Res. 25: 101-103
Mackay D, Di Guardo A, Paterson S, Kicsi G, Cowan CE & Kane DM (1996) Assessment of chemical fate in the environment using evaluative, regional and local-scale models: illustrative applications to chlorobenzene and linear alkylbenzene sulfonates. Environ. Toxicol. Chem. 15(9): 1638-1648
Magoarou P (2000) Urban wastewater in Europe-what about the sludge? Proceedings of the EU workshop-on problems around sludge, 28-19 November 1999, Stresa, Italy, 9-16
Mogensen AS & Ahring BK (2002) Formation of metabolites during biodegradation of linear alkylbenzene sulfonate in an up-flow anaerobic sludge bed reactor under thermophilic conditions. Biotechnol. Bioengng 77(5): 483-488
Mogensen AS, Haagensen F & Ahring BK (2002) Anaerobic degradation of Linear Alkylbenzene Sulfonate. Environmental Toxicology and Chemistry, accepted for publication
Rueter P, Rabus R, Wilkes H, Aeckersberg F, Rainey FA, Jannasch HW & Widdel F (1994) Anaerobic oxidation of hydrocarbons in crude oil by new types of sulphate-reducing bacteria. Nature 372: 455-458
Schulze K (1996) Der westereuropäische Tensidmarkt 1994/1995, Tenside Surf. Det. 33:94-95
So Chi Ming & Young LY (1999) Isolation and characterization of a sulfate-reducing bacterium that anaerobically degrades alkanes. Appl. Environ. Microbiol. 65: 2969-2976
Takada H, Mutoh K, Tomita N, Miyadzu T & Ogura N (1994) Rapid removal of Linear Alkylbenzene Sulfonates (LAS) by attached biofilm in an urban shallow stream. Wat. Res. 28: 1953-1960
Van der Meeren P & Verstraete W (1996) Surfactants in relation to bioremediation and wastewater treatment. Current Opinion in Colloid and Interface Science 1(5): 624-634
Wilson BH, Smith GB & Rees JF (1986) Biotransformation of selected alkylbenzenes and halogenated aliphatic hydrocarbons in methanogenic aquifer material: a microcosm study. Envir. Sci. Technol. 20: 997-1002
Wolin EA, Wolin MJ & Wolfe RS (1963) Formation of methane bacterial extracts. J Biol Chem 238: 2882-2886
Zeyer J, Kuhn EP & Schwarzenbach RP (1986) Rapid microbial mineralization of toluene and 1,3-dimethylbenzene in the absence of molecular oxygen. Appl. Envir. Microbiol. 52: 944-947
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Gavala, H.N., Ahring, B.K. Inhibition of the anaerobic digestion process by linear alkylbenzene sulfonates. Biodegradation 13, 201–209 (2002). https://doi.org/10.1023/A:1020860027176
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DOI: https://doi.org/10.1023/A:1020860027176