Abstract
Peptides corresponding to residues 75–86 (RPQQPYPQPQPQ) and 75–85 of the A-gliadin structure, which were shown to be active in an animal model of celiac disease, were digested in vitro with small intestinal mucosa from children with celiac disease in remission and with mucosa from normal children. The products of digestion were separated into two fractions by gel permeation chromatography. Undigested residues (Mr > 400 fraction) from both peptides contained mainly glutamine, proline, and tyrosine, while the digested materials (Mr < 400 fraction) contained mainly proline, glutamine and arginine. Much larger amounts of undigested peptides were obtained from digestion with celiac mucosa than from normal mucosa. The results with peptide 75–86 indicated that the octapeptide 77–84 (QQPYPQPQ) was the main residual component and this peptide was shown to be active in the assay. Peptide 77–84 was also obtained as a residue from digestion of peptide 75–85, together with heptapeptide 77–83. The results lend further support for a primary mucosal defect in celiac disease and indicate that residual peptides in the small intestine of patients with the disease still retain appreciable toxicity.
Similar content being viewed by others
REFERENCES
Auricchio, S. (1991). In Coeliac Disease--40 Years Gluten Free (Mearin, M. L., and Mulder, C. J. J., eds.), Kluwer, Dordrecht, pp. 21–26.
Cornell H. J. (1988). Clin. Chim. Acta 176, 279–290.
Cornell, H. J., and Mothes, T. (1993). Biochim. Biophys. Acta 1181, 169–173.
Cornell, H. J., and Mothes, T. (1995). Biochim. Biophys. Acta 1270, 168–172.
Cornell, H. J., and Rivett, D. E. (1995). J. Protein Chem. 14, 335–339.
Cornell, H. J., and Rolles, C. J. (1978). Gut 19, 253–259.
Cornell, H. J., Auricchio, R. S., De Ritis, G., De Vincenze, M., Maiuri, L., Raia, V., and Silano, V. (1988). Pediatr. Res. 24, 233–237.
Cornell, H. J., Wieser, H., and Belitz, H-D. (1992). Clin. Chim. Acta 213, 37–50.
Cornell, H. J., Skerritt, J. H, Puy, R., and Javadpour, M. (1994). Biochim. Biophys. Acta 1226, 126–130.
De Ritis, G., Occorsio, P., Auricchio, S., Gramenzi, F., Morisi, G., and Silano, V. (1979). Pediatr. Res. 13, 1255–1261.
De Ritis, G., Auricchio, S., Jones, H. W., Lew, E. J.-L., Bernardin, J. E., and Kasarda, D. D. (1988). Gastroenterology 94, 41–49.
Frazer, A. C., Fletcher, R. F., Ross, C. A. C, Shaw, B., Sammons H. G., and Schneider, R. (1959). Lancet 2, 252–255.
Graf, L., Horvath, K., Walcz, E., Berzetei, I., and Burnier, J. (1987). Neuropeptides 9, 113–122.
Kocna, P., Mothes, T., Krchnak, V., and Fric, P. (1991). Z. Lebensm. Unters. Forsch. 192, 116–119.
Mothes, T., Mühle, W., Müller, F., and Hekkens, W. T. J. M. (1985). Biol. Neonate 48, 59–64.
Sturgess, R., Day, P., Ellis, H. J., Lundin, K. E. A., Gjertsen, H. A., Kontakov, M., and Ciclitira, P. (1994). Lancet 343, 758–761.
Troncone, R. (1992). In Report of 7th Meeting of Working Group on Prolamin Analysis and Toxicity, November 4–6, pp. 39–42.
Walz, F., Wieser, H., and Stern, M. (1996). Scand. J. Gastroenterol. 31, 240–246.
Wieser, H., Belitz, H.-D., Idar, D., and Ashkenazi, A. (1986). Z. Lebensm. Unters. Forsch. 182, 115–117.
Zioudrou, C., Streaty, R. A., and Klee, W. A. (1979). J. Biol. Chem. 254, 2446–2449.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cornell, H.J. Partial In Vitro Digestion of Active Gliadin-Related Peptides in Celiac Disease. J Protein Chem 17, 739–744 (1998). https://doi.org/10.1023/A:1020765932203
Published:
Issue Date:
DOI: https://doi.org/10.1023/A:1020765932203