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Salinity stress-tolerant and -sensitive rice (Oryza sativa L.) regulate AKT1-type potassium channel transcripts differently

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Abstract

In the indica rice (Oryza sativa L.) a cDNA was characterized that encoded OsAKT1 homologous to inward-rectifying potassium channels of the AKT/KAT subfamily. Transcript analysis located OsAKT1 predominantly in roots with low abundance in leaves. Cell-specificity of OsAKT expression was analyzed by in situ hybridizations. In roots, strongest signals were localized to the epidermis and the endodermis, whereas lower transcript levels were detected in cells of the vasculature and the cortex. In leaves, expression was detected in xylem parenchyma, phloem, and mesophyll cells. Transcriptional regulation and cell specificity of OsAKT1 during salt stress was compared in rice lines showing different salinity tolerance. In the salt-tolerant, sodium-excluding varieties Pokkali and BK, OsAKT1 transcripts disappeared from the exodermis in plants treated with 150 mM NaCl for 48 h but OsAKT1 transcription was not repressed in these cells in the salt-sensitive, sodium-accumulating variety IR29. Significantly, all lines were able to maintain potassium levels under sodium stress conditions, while sodium concentrations in the leaves of IR29 increased 5–10-fold relative to the sodium concentration in BK or Pokkali. The divergent, line-dependent and salt-dependent, regulation of this channel does not significantly affect potassium homeostasis under salinity stress. Rather, repression in Pokkali/BK and lack of repression in IR29 correlate with the overall tolerance character of these lines.

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Author notes

  1. Hans J. Bohnert

    Present address: Departments of Plant Biology and Crop Sciences, University of Illinois, ERML 196, 1201 W. Gregory Drive, Urbana, IL, 61801, USA

Authors and Affiliations

  1. Germany

    Dortje Golldack, Francoise Quigley & Hans J. Bohnert

  2. Lehrstuhl für Stoffwechselphysiologie und Biochemie der Pflanzen, Universität Bielefeld, Bielefeld, Germany

    Dortje Golldack

  3. Laboratoire de Genétique Moléculaire des Plantes, UMR CNRS 5575, Université Joseph Fourier, Grenoble, France

    Francoise Quigley

  4. Department of Biochemistry, University of Arizona, Tucson, AZ, 85721, USA

    Christine B. Michalowski & Uma R. Kamasani

  5. Department of Plant Sciences, USA

    Hans J. Bohnert

  6. Department of Molecular and Cellular Biology, University of Arizona, Tucson, AZ, 85721, USA

    Hans J. Bohnert

Authors
  1. Dortje Golldack
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  2. Francoise Quigley
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  3. Christine B. Michalowski
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  4. Uma R. Kamasani
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  5. Hans J. Bohnert
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Correspondence to Hans J. Bohnert.

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Golldack, D., Quigley, F., Michalowski, C.B. et al. Salinity stress-tolerant and -sensitive rice (Oryza sativa L.) regulate AKT1-type potassium channel transcripts differently. Plant Mol Biol 51, 71–81 (2003). https://doi.org/10.1023/A:1020763218045

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