Abstract
The term “pseudomutagens” is suggested for factors that increase the spontaneous mutation level not by induction of DNA molecule changes but as a result of inhibition of the repair of spontaneous pre-mutation lesions. The expected regularities of pseudomutagen effects, which are suitable as criteria for their identification, are considered. The experimental results on the effects of six modifiers of cell metabolism in human lymphocytes are analyzed. Four of the modifiers (5-fluorodeoxyuridine, sodium cyanide, sodium fluoride and monoiodoacetic acid) behaved as pseudomutagens according to all the criteria applied. 2,4-Dinitrophenol proved to be a true mutagen. Adenosine diphosphate revealed unusual properties: it inhibited the repair of spontaneous lesions but decreased the frequency of structural mutations induced by radiation. The significance of the phenomenon of the pseudomutagenesis for the problem of environmental mutagens/carcinogens is discussed.
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REFERENCES
Luchnik, N.V., The Phenomenon of False Mutagenesis, Tezisy dokladov V Sovetsko-Amerikanskogo simpoziuma (Pushchino-Baku, mai-iyun', 1978 g.) (Proc. V Sov.— Am. Symp. (Pushchino-Baku, May—June, 1978), Moscow, 1978, p. 23.
Fesenko, E.V. and Luchnik, N.V., The Pseudomutagenic Effect Produced by Metabolic Inhibitors in Crepis capillaris, Materialy XIV Ezhegodnoi konferentsii Evropeiskogo obshchestva po mutagenam vneshnei sredy (Proc. XIV Ann. Conf. of Eur. Soc. Environmental Mutagens), Moscow, 1984, pp. 165-166.
Luchnik, N.V. and Ovchinnikova, V.G., The Effect of DNA Synthesis Inhibitors Applied at Various Cell-Cycle Stages on the Frequency of Radiation-Induced Chromosome Aberrations, in Molekulyarnye mekhanizmy genet-icheskikh protsessov (Molecular Mechanisms of Genetic Processes), Moscow: Nauka, 1976, pp. 192-199.
Poryadkova, N.A., Izmailova, N.N., and Luchnik, N.V., Generation of Chromosome Aberrations in Human Lymphocytes Exposed to 5-Fluorodeoxyuridine Alone or in Combination with g-Irradiation: Changes of the Effect in the Course of the Cell Cycle, Genetika (Moscow), 1981, vol. 17, no. 12, pp. 2212-2219.
Luchnik, N.V., Poryadkova, N.A., and Izmailova, N.N., The Effect of Cell Respiration Inhibitors on Generation of Structural Mutations in Human Lymphocytes Irradiated at Various Stages of the Cell Cycle, Genetika (Moscow), 1985, vol. 21, no. 2, pp. 252-261.
Luchnik, N.V. and Sevankaev, A.V., Radiation-Induced Chromosomal Aberrations in Human Lymphocytes: 1. Dependence on the Dose of γ-Rays and an Anomaly at Low Doses, Mutat. Res., 1976, vol. 36, no. 2, pp. 363-378.
Luchnik, N.V., The Chromosome Cycle of DNA, Studia Biophys., 1971, vol. 27, no. 2, pp. 157-164.
Luchnik, N.V., Generation of Chromosome Aberrations in Cells Irradiated at Various Stages of the Cell Cycle, Radiobiologiya, 1973, vol. 13, no. 2, pp. 163-177.
Luchnik, N.V., Genetic Information Exchange in the Cell Cycle, Dokl. Akad. Nauk SSSr, 1973, vol. 212, no. 4, pp. 985-988.
Poryadkova, N.A., Kuz'mina, E.G., and Luchnik, N.V., Spontaneous Chromosome Aberrations in Clones Obtained from HEp-2 Cells, Vopr. Onkol., 1973, vol. 19, no. 3, pp. 80-86.
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Luchnik, N.V. Phenomenon Of Pseudomutagenesis: Experiments with Modifiers of Cell Metabolism in Human Lymphocytes. Russian Journal of Genetics 38, 1133–1139 (2002). https://doi.org/10.1023/A:1020692502428
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DOI: https://doi.org/10.1023/A:1020692502428