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Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding

Abstract

Inter simple sequence repeat (ISSR)-PCR is a technique, which involvesthe use of microsatellite sequences as primers in a polymerase chainreaction to generate multilocus markers. It is a simple and quick methodthat combines most of the advantages of microsatellites (SSRs) andamplified fragment length polymorphism (AFLP) to the universality ofrandom amplified polymorphic DNA (RAPD). ISSR markers are highlypolymorphic and are useful in studies on genetic diversity, phylogeny, genetagging, genome mapping and evolutionary biology. This review providesan overview of the details of the technique and its application in geneticsand plant breeding in a wide range of crop plants.

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Correspondence to N. Sarla.

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Pradeep Reddy, M., Sarla, N. & Siddiq, E. Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica 128, 9–17 (2002). https://doi.org/10.1023/A:1020691618797

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  • anchored primer
  • DNA marker
  • genome mapping
  • gene tagging
  • genetic diversity
  • ISSR-PCR