Molecular and Cellular Biochemistry

, Volume 240, Issue 1–2, pp 1–8 | Cite as

The physiological scavenger receptor function of hepatic sinusoidal endothelial and Kupffer cells is independent of scavenger receptor class A type I and II

  • Berit Hansen
  • Beatriz Arteta
  • Bård Smedsrød
Article

Abstract

This study was undertaken to determine the role of scavenger receptor class A type I and II (SR-AI/II) in the physiological scavenger function of hepatic sinusoidal endothelial cells (SEC) and Kupffer cells (KC). Following intravenous administration of radiolabelled SR-ligands, [advanced glycation end (AGE)-products, N-terminal propeptide of type III procollagen (PIIINP) and formaldehyde treated serum albumin (FSA)] in SR-AI/II-deficient and wild-type mice, radioactivity was removed equally rapidly from the circulation of both types of mice. The major site of uptake was the liver. Separation of liver cells showed that the population of SEC and KC were responsible for ∼55 and ∼25% of the uptake. There was no difference in plasma clearance, organ distribution or cell distribution in SR-AI/II-deficient and wild-type mice. Experiments performed to determine the specificity of endocytosis in cultured SEC showed that uptake of radiolabelled SR-ligands (AGE-protein, PIIINP or FSA) was inhibited equally well by unlabelled FSA and AGE-protein in SEC from receptor deficient and wild-type mice. We conclude from these findings that SR-AI/II is of minor importance in the plasma clearance of physiological as well as foreign SR-ligands.

hepatic sinusoidal endothelial cells advanced glycation end products N-terminal propeptide of type III procollagen receptor mediated endocytosis plasma clearance scavenger receptor 

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Berit Hansen
    • 1
    • 2
  • Beatriz Arteta
    • 1
    • 3
  • Bård Smedsrød
    • 1
  1. 1.Department of Experimental Pathology, Institute of Medical BiologyUniversity of TromsøTromsøNorway
  2. 2.Department of DermatologyUniversity Medical Center Mannheim, Ruprecht-Karls University HeidelbergMannheimGermany
  3. 3.Department of Cellular Biology and Morphological SciencesUniversity of Basque CountryVizcayaSpain

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