Abstract
Protein sequence analysis using an adsorptive biphasic sequencing cartridge, a set of two coupled columns introduced by Hewlett-Packard for protein sequencing by Edman degradation, in an Applied Biosystems 473A protein sequencer has been demonstrated. Samples containing salts, detergents, excipients, etc. (e.g., formulated protein drugs) can be easily analyzed using the ABI sequencer. Simple modifications to the ABI sequencer to accommodate the cartridge extend its utility in the analysis of difficult samples. The ABI sequencer solvents and reagents were compatible with the HP cartridge for sequencing. Sequence information up to ten residues can be easily generated by this nonoptimized procedure, and it is sufficient for identifying proteins by database search and for preparing a DNA probe for cloning novel proteins.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Miller, C. G. (1994). Adsorptive biphasic column technology for protein sequence analysis and protein chemical modification, Methods 6:315–333.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tang, S., Mozdzanowski, J. & Anumula, K.R. Protein Sequence Analysis Using Hewlett-Packard Biphasic Sequencing Cartridges in an Applied Biosystems 473A Protein Sequencer. J Protein Chem 18, 75–77 (1999). https://doi.org/10.1023/A:1020651500943
Published:
Issue Date:
DOI: https://doi.org/10.1023/A:1020651500943