Abstract
The stability of calf skin collagen (CSC) type I during thermal and chemical denaturation in the presence of glycerol was investigated. Thermal denaturation of type I collagen was performed in the presence of glycerol or in combination with urea and sodium chloride. The denaturation curves obtained in the presence of urea or sodium chloride retained their original shape without glycerol. These curves were shifted upward proportionally to the glycerol concentration in the reaction medium. This means that glycerol and the denaturants act independently. The explanation is based on the difference in the mechanism of their action on the collagen molecule.
Similar content being viewed by others
REFERENCES
Arakawa, T., and Timasheff, S. N. (1985). Biochemistry 47, 411-414.
Bachinger, H. P., and Morris, N. P. (1990). Matrix 10, 331-338.
Danielsen, C. C. (1982). Colloids Related Res. 2, 143-150.
Dyerberg, J., and Hjorne, N. (1966). Klin. Wochenschr. 44, 267-273.
Fietzek, P. P., and Kuhn, K. (1976). Int. Rev. Connect. Tiss. Res. 7, 1-60.
Fuji, T., and Kuhn, K. (1975). Hoppe-Seyler Z. Physiol. Chem. 356, 1793-1801.
Furthmayr, H., and Timpl, R. (1971). Anal. Biochem. 41, 510-516.
Gekko, K., and Koga, Sh. (1983). J. Biochem. 94, 199-205.
Gekko, K., and Timasheff, S. N. (1981). Biochemistry, 20, 4667-4676.
Hayashi, T., and Nagai, Y. (1972). J. Biochem. 72, 749-758.
Komsa-Penkova, R., Goshev, I., and Alexandrova, Tz. (1995). Sci. Works Univ. Pleven (Bulgaria) 15, 5-8.
Lim, V. J. (1981). FEBS Lett. 32, 1-5.
Na, G. C. (1986). Biochemistry 25, 967-973.
Santoro, M. M., Liu, Y., Khan, S. M. A., Hou, L., and Bolen, D. W. (1992). Biochemistry 31, 5278-5283.
Yancey, P. H., and Burg M. B. (1990). Am. J. Physiol. 258, R198-R204.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Penkova, R., Goshev, I., Gorinstein, S. et al. Stability of Collagen During Denaturation. J Protein Chem 18, 397–401 (1999). https://doi.org/10.1023/A:1020632424142
Published:
Issue Date:
DOI: https://doi.org/10.1023/A:1020632424142