Abstract
1. Cypridina luciferin analogues, 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one (MCLD) and 2-methyl-6-phenyl-3,7-dihydroimidazo[1,2-a]pyrazin-3-one(CLA), react with O2 − or 1O2 to emit light in visible region. Such chemiluminescences were used for the detection of O2 − or 1O2 in activated leukocyte systems and myeloperoxidase (granulocyte-extract) + Br− + H2O2 systems in vitro.
2. The mechanism of MCLA (CLA)-dependent luminescence is described in detail. Superoxide generated from sinusoidal cells in acute ethanol intoxication of rats was detected by MCLA-dependent luminescence from the surface of perfused rat liver (organ luminescence).
3. Furthermore, with alive animals, O2 − generated in the lung of rats with necrotized pancreatitis and that in the stomach of rats after ischemia/reperfusion were detected by their organ luminescences.
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Nakano, M. Detection of Active Oxygen Species in Biological Systems. Cell Mol Neurobiol 18, 565–579 (1998). https://doi.org/10.1023/A:1020621616389
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DOI: https://doi.org/10.1023/A:1020621616389