Food products fermented by lactic acid bacteria have long been used for their proposed health promoting properties. In recent years, selected probiotic strains have been thoroughly investigated for specific health effects. Properties like relief of lactose intolerance symptoms and shortening of rotavirus diarrhoea are now widely accepted for selected probiotics. Some areas, such as the treatment and prevention of atopy hold great promise. However, many proposed health effects still need additional investigation. In particular the potential benefits for the healthy consumer, the main market for probiotic products, requires more attention. Also, the potential use of probiotics outside the gastrointestinal tract deserves to be explored further. Results from well conducted clinical studies will expand and increase the acceptance of probiotics for the treatment and prevention of selected diseases.
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Aso Y, Akaza H, Kotake T, Tsukamoto T, Imai K, Naito S&BLP Study Group (1995) Preventive effect of a Lactobacillus casei preparation on the recurrence of superficial bladder cancer in a double-blind trial. Eur. J. Urol. 27: 104–109.
Barefoot SF&Ratnam P (1998) Composition for treating acne. US patent WO98/10743.
Benno Y, Mitsuoka T& Kanazawa K (1991) Human faecal flora in health and colon cancer. Acta Chirurgica Scand. 521: 15–23.
Benno Y, He F, Hosoda M, Hashimoto H, Kojima T, Yamazaki K, Iino H, Mykkänen H& Salminen S (1996) Effects of Lactobacillus GG yogurt on human intestinal microecology in Japanese subjects. Nutrition Today 31: 9S-11S.
Black FT, Andersen PL, Örsskov J, Örskov F, Gaarslev K& Laulund S (1989) Prophylactic efficacy of lactobacilli on travelers diarrhea. Travel Med. 7: 333–335.
Black F, Einarsson K, Lidbeck A, Orrhage K& Nord CE (1991) Effect of lactic acid producing bacteria on the human intestinal microflora during ampicillin treatment. Scand. J. Infect. Dis. 2: 247–254.
Brandtzaeg P (1995) Molecular and cellular aspects of the secretory immunoglobulin system. APMIS 103: 1–19.
Brigidi P, Vitali B, Swennen E, Bazzocchi G& Matteuzzi D (2001) Effects of probiotic administration upon the composition and enzymatic activity of human fecal microbiotia in patients with irritable bowel syndrome or functional diarrhea. Res. Microbiol. 152: 735–741.
Bunthof CJ, Bloemen K, Breeuwer P, Rombouts FM& Abee T (2001) Flow cytometric assessment of viability of lactic acid bacteria. Appl. Environ. Microbiol. 67: 2326–2335.
Cangemi de Gutierrez R, Santos V& Nader-Macías, ME (2001) Protective effect of intranasally inocculated Lactobacillus fermentum against Streptococcus pneumoniae challenge on the mouse respiratory tract. FEMS Immunol. Med. Microbiol. 31: 187–195.
Claeson M& Merson MH (1990) Global progress in the control of diarrheal disease. Pediatr. Infect. Dis. J. 9: 345–355.
Ducroc R, Heyman M, Beaufrere B, Morgat JL& Desjeux JF (1983) Horseradish peroxidase transport across rabbit jejunum and Peyer's patches in vitro. Am. J. Physiol. 245: G54–G58.
Elo S, Saxelin M& Salminen S (1991) Attachment of Lactobacillus casei strain GG to human colon carcinoma cell line Caco-2: comparison with other dairy strains. Lett. Appl. Microbiol. 13: 154–156.
Felley CP, Corthesy-Theulaz I, Rivero JL, Sipponen P, Kaufmann M, Bauerfeind P, Wiesel PH, Brassart D, Pfeifer A, Blum AL& Michetti P (2001) Favourable effect of an acidified milk (LC-1) on Helicobacter pylori gastritis in man. Eur. J. Gastroenterol. Hepatol. 13: 25–29.
Fuller R (1989) Probiotics in man and animals. J. Appl. Bacteriol. 66: 365–378.
Gill HS& Rutherfurd KJ (2001) Probiotic supplementation to enhance natural immunity in the elderly: effects of a newly characterized immunomodulatory strain Lactobacillus rhamnosus HN001 (DR20™) on leucocyte phagocytosis. Nutr. Res. 21: 183–189.
Gismondo MR, Drago L& Lombardi A (1999) Review of probiotics available to modify gastrointestinal flora. Int. J. Antimicrobial Agents 12: 287–292.
Goldin BR (1998) Health benefits of probiotics. Br. J. Nutr. 80: S203–S207.
Greenwald P, Clifford CK& Milner JA (2001) Diet and cancer prevention. Eur. J. Cancer 37: 948–965.
Grönlund MM, Arvilommi H, Kero P, Lehtonen OP& Isolauri E (2000) Importance of intestinal colonisation in the maturation of humoral immunity in early infancy: a prospective follow up study of healthy infants aged 0-6 months. Arch. Dis. Childhood 83: F186–F192.
Groux H& Powrie F (1999) Regulatory T cells and inflammatory bowel disease. Immunol. Today 20: 442–446.
Guandalini S, Pensabene L, Zikri MA, Dias JA, Casali LG, Hoekstra H, Kolacek S, Massar K, Micetic-Turk D, Papadopoulou A, de Sousa JS, Sandhu B, Szajewska H& Weizman Z (2000) Lactobacillus GG administered in oral rehydration solution to children with acute diarrhea: a multicenter European trial. J. Ped. Gastroenterol. Nutr. 30: 54–60.
Guarner F& Schaafsma GJ (1998) Probiotics. Int. J. Food Microbiol. 39: 237–238.
Gupta P, Andrew H, Kirschner BS& Guandalini S (2000) Is Lactobacillus GG helpful in children with Crohn's disease? Results of a preliminary, open-label study. J. Ped. Gastroenterol. Nutr. 31: 453–457.
Guslandi M, Mezzi G, Sorghi M& Testoni PA (2000) Saccharomyces boulardii in maintenance treatment of Crohn's disease. Digestive Dis. Sci. 45: 1462–1464.
Haller D, Bode C, HammesWP, Pfeifer AMA, Schiffrin EJ& Blum S (2000) Non-pathogenic bacteria elicit differential cytokine response by intestinal epithelial cell/Leukocyte co-cultures. Gut 47: 79–87.
Hamilton-Miller JMT (2001) A review of clinical trials of probiotics in the management of inflammatory bowel diesease. Infect. Dis. Rev. 3: 83–87.
Hamilton-Miller JMT& Shah S (2002) Deficiencies in microbiological quality and labellinf of probiotic supplements. Int. J. Food Microbiol. 72: 175–176.
Hatakka K, Savilahti E, Pönkä A, Meurman JH, Poussa T, Näse L, Saxelin M& Korpela R (2001) Effect of long term consumption of probiotic milk on infections in children attending day care centres: double blind randomised trial. Br. Med. J. 322: 1327–1329.
Helgeland L, Vaage JT, Rolstad B, Midtvedt T& Brandtzaeg P (1996) Microbial colonization influences composition and T-cell receptor V beta repertoire of intraepithelial lymphocytes in rat intestine. Immunology 89: 494–501.
Heyman M& Desjeux JF (2000) Cytokine-induced alteration of the epithelial barrier to food antigens in disease. Ann. NY Acad. Sci. 915: 304–311.
Hilton E, Isenberg HD, Alperstein P, France K& Borenstein MT (1992) Ingestion of yoghurt containing Lactobacillus acidophilus as prophylaxis for candidal vaginitis. Ann. Int. Med. 116: 353–357.
Hirayama K& Rafter J (2000) The role of probiotic bacteria in cancer prevention. Microbes Infect. 2: 681–686.
Isolauri E (1999) Probiotics and gut inflammation. Curr. Opin. Gastroenterol. 15: 534–537.
Isolauri E (2001) Probiotics in human disease. Am. J. Clin. Nutr. 73: S1142–S1146.
Isolauri E, Kaila M, Arvola T, Majamaa H, Rantala I, Virtanen E& Arvilommi H (1993) Diet during rotavirus enteritis affects jejunal permeability to macromolecules in suckling rats. Ped. Res. 33: 548–553.
Isolauri E, Arvola T, Sütas Y, Moilanen E& Salminen S (2000) Probiotics in the management of atopic eczema. Clin. Exp. Allergy 30: 1605–1610.
Isolauri E, Sütas Y, Kankaanpää P, Arvilommi H& Salminen S (2001) Probiotics: effects on immunity. Am. J. Clin. Nutr. 73: S444–S445.
Kaila M, Isolauri E, Soppi E, Virtanen E, Laine S& Arvilommi H (1992) Enhancement of the circulating antibody secreting cell response in human diarrhea by a human Lactobacillus strain. Ped. Res. 32: 141–144.
Kaila M, Isolauri E, Saxelin M, Arvilommi H& Veskari T (1995) Viable versus inactivated Lactobacillus strain GG in acute rotavirus diarrhoea. Arch. Dis. Childhood 72: 51–53.
Kalliomäki M, Kirjavainen P, Eerola E, Kero P, Salminen S& Isolauri E (2001a) Distinct patterns of neonatal gut microflora in infants in whom atopy was and was not developing. J. Allergy Clin. Immunol. 107: 129–134.
Kalliomäki M, Salminen S, Arvilommi H, Kero P, Koskinen P& Isolauri E (2001b) Probiotics in primary prevention of atopic disease: a randomised placebo-controlled trial. Lancet 357: 1076–1079.
Lasser RB, Bond JH& Levitt MD (1975) The role of intestinal gas in functional abdominal pain. New Engl. J. Med. 293: 524–526.
Launiala K (1968) The effect of unabsorbed sucrose and mannitol on the small intestinal flow rate and mean transit time. Scand. J. Gastroenterol. 3: 665–671.
Lee Y-K, Nomoto K, Salminen S& Gorbach SL (1999) Handbook of Probiotics. John Wiley&Sons, Inc., New York.
Link-Amster H, Rochat F, Saudan KY, Mignot O, Aeschlimann JM (1994) Modulation of a specific humoral immune response and changes in intestinal flora mediated through fermented mik intake. FEMS Immunol. Med. Microbiol. 10: 55–64.
MacDonald T (2001) The reaction of the immune system to pathogens but not food antigens and commensal bacteria. Seminars Immunol. 13: 159–161.
Majamaa H& Isolauri E (1997) Probiotics: a novel approach in the management of food allergy. J. Allergy Clin. Immunol. 99: 179–186.
Malchow HA (1997) Crohn's disease and Echerichia coli. J. Clin. Gastroenterol. 25: 653–658.
Mattila-Sandholm T, Blum S, Collins JK, Crittenden R, de Vos W, Dunne C, Fondén R, Grenov G, Isolauri E, Kiely B, Marteau P, Morelli L, Ouwehand A, Reniero R, Saarela M, Salminen S, Saxelin M, Schiffrin E, Shanahan F, Vaughan E& von Wright A (1999) Probiotics: towards demonstrating efficacy. Trends Food Sci. Technol. 10: 393–399.
Meurman JH, Antila H& Salminen S (1994) Recovery of Lactobacillus strain GG (ATCC 53103) from saliva of healthy volunteers after consumption of yoghurt prepared with the bacterium. Microbial Ecol. Health Dis. 7: 295–298.
Nagao F, Nakayama M, Muto T& Okumura K (2000) Effects of a fermented milk drink containing Lactobacillus casei strain Shirota on the immune system in healthy human subjects. Biosci. Biotechnol. Biochem. 64: 2706–2708.
Niedzielin K, Kordecki H& Birkenfeld B (2001) A controlled, double-blind, randomized study on the efficacy of Lactobacillus plantarum 299v in patients with irritable bowel syndrome. Eur. J. Gastroenterol. Hepatol. 13: 1143–1147.
Ouwehand AC& Salminen SJ (1998) The health effects of cultured milk products with viable and non-viabel bacteria. Int. Dairy J. 8: 749–758.
Ouwehand AC, Isolauri E, Kirjavainen PV& Salminen SJ (1999) Adhesion of four Bifidobacterium strains to human intestinal mucus from subjects in different age groups. FEMS Microbiol. Lett. 172: 61–64.
Ouwehand AC, Tölkkö S& Salminen S (2001) The effect of digestive enzymes on the adhesion of probiotic bacteria in vitro. J. Food Sci. 66: 856–859.
Ouwehand AC, Salminen S, Tölkkö S, Roberts PJ, Ovaska J&Salminen E (2002) Disease dependent adhesion of lactic acid bacteria to colonic tissue in vitro. Microecol. Ther. In press.
Pessi T, Isolauri E, Sütas Y, Kankaanranta H, Moilanen E& Hurme M (2000a) Suppression of T cell activation by Lactobacillus rhamnosus GG-degraded bovine casein. Immunopharmacology 1: 211–218.
Pessi T, Sütas Y, Hurme M& Isolauri E (2000b) Interleukin-10 generation in atopic children following oral Lactobacillus rhamnosus GG. Clin. Exp. Allergy 30: 1804–1808.
Petti S, Tarsitani G& Simonetti D'Arca A (2001) A randomized clinical trial of the effect of yoghurt on the human salivary microflora. Arch. Oral Biol. 46: 705–712.
Redondo-Lopez V, Cook RL& Sobel JD (1990) Emerging role of lactobacilli in the control and maintenance of the vaginal bacterial microflora. Rev. Infect. Dis. 12: 856–872.
Reid G, Bruce AW& Taylor M (1992) Influence of three day antimicrobial therapy and Lactobacillus vaginal suppositories on recurrence of urinary tract infections. Clin. Therapy 14: 11–16.
Reid G (2001) Probiotic agents to protect the urogenital tract against infection. Am. J. Clin. Nutr. 73: 437S–443S.
Reid G, Bruce AW, Fraser N, Heinemann C, Owen J& Henning B (2001) Oral probiotics can resolve urogenital infections. FEMS Immunol. Med. Microbiol. 30: 49–52.
Saavedra JM, Bauman NA, Oung I, Perman JA& Yolken RH (1994) Feeding of Bifidobacterium bifidum and Streptococcus thermophilus to infants in hospital for prevention of diarrhoea and shedding of rotavirus. Lancet 344: 1046–1049.
Salim AF, Phillips AD& Farthing MJ (1990) Pathogenesis of gut virus infection. Baillières Clin. Gastroenterol. 4: 593–607.
Salminen S, Isolauri E& Salminen E (1996) Clinical uses of probiotics for stabilizing the gut mucosal barrier: successful strains and future challenges. Antonie Van Leeuwenhoek 70: 347–358.
Salminen S, Bouley C, Boutron-Ruault M-C, Cummings JH, Franck A, Gibson GR, Isolauri E, Moreau M-C, Roberfroid M& Rowland I (1998) Functional food science and gastrointestinal physiology and function. Br. J. Nutr. 80: S147–S171.
Salminen S, Ouwehand A, Benno Y& Lee YK (1999) Probiotics: how should they be defined? Trends Food Sci. Technol. 10: 107–110.
Sanderson IR& Walker WA (1993) Uptake and transport of macromolecules by the intestine: possible role in clinical disorders (an update). Gastroenterology 104: 622–639.
Shimoyama T, Hori S, Tamura K, Yamamura M, Tanaka M& Yamazaki K (1984) Microflora of patients with stool abnormality. Bifidobacteria and Microflora 3: 35–42.
Shornikova A-V, Casas I, Mykkänen H, Salo E& Vesikari T (1997) Bacteriotherapy with Lactobacillus reuteri in rotavirus gastroenteritis. Ped. Infect. Dis. J. 16: 1103–1107.
Spanhaak S, Havenaar R& Schaafsma G (1998) The effect of consumption of milk fermented by Lactobacillus casei strain Shirota on the intestinal microflora and immune parameters in humans. Eur. J. Clin. Nutr. 52: 899–907.
Strachan DP (1989) Hay fever, hygiene, and household size. Br. Med. J. 299: 1259–1260.
Sudo N, Sawamura S, Tanaka K, Aiba Y, Kubo C& Koga Y (1997) The requirement of intestinal bacterial flora for the development of an IgE production system fully susceptible to oral tolerance induction. J. Immunol. 159: 1739–1745.
Sugita T& Togawa M (1994) Efficacy of Lactobacillus preparation Biolactis powder in children with rotavirus enteritis. Jpn. J. Pediatr. 47: 2755–2762.
Surawicz CM, Elmer GW, Spleeman P, McFarland LV, Chinn J& van Belle G (1989) Prevention of antibiotic associated diarrhoea by Saccharomyces boulardii: a prospective study. Gastroenterology 96: 981–988.
Sütas Y, Hurme M& Isolauri E (1996) Downregulation of antiCD3 antibody-induced IL-4 production by bovine caseins hydrolysed with Lactobacillus GG-derived enzymes. Scand. J. Immunol. 43: 687–689.
Temmerman R, Huys G, Pot B&Swings J (2001) Identification and antibiotic resistance of isolates from probiotic products. Abstracts of 101st ASM General Meeting, C–289.
Tuomola EM, Ouwehand AC& Salminen SJ (2000) Chemical, physical and enzymatic pre-treatments of probiotic lactobacilli alter their adhesion to human intestinal mucus glycoproteins. Int. J. Food Microbiol. 60: 75–81.
Tuomola E, Crittenden R, Playne M, Isolauri E& Salminen S (2001) Quality assurence criteria for probiotic bacteria. Am. J. Clin. Nutr. 73: S393–S398.
Vesa T, Marteau P& Korpela R (2000) Lactose intolerance. J. Am. Coll. Nutr. 19: 165S–175S.
Virta M, Lineri S, Kankaanpää P, Karp M, Peltonen K, Nuutila J& Lilius E-M (1998) Determination of complement-mediated killing of bacteria by viability staining and bioluminescence. Appl. Environ. Microbiol. 64: 515–519.
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Ouwehand, A.C., Salminen, S. & Isolauri, E. Probiotics: an overview of beneficial effects. Antonie Van Leeuwenhoek 82, 279–289 (2002). https://doi.org/10.1023/A:1020620607611
- health effects
- lactic acid bacteria