Abstract
The quality of nucleic acid solution structures can be significantly improved using residual dipolar coupling data. However, many of the one-bond couplings that could be used for this purpose are difficult to measure. Conventional 2D experiments are often unable to reveal one-bond H2′-C2′ and H3′-C3′ couplings in large RNA molecules due to spectral overlap. Here we show how to use 3D HCcH-COSY and Relay HCcH-COSY to measure one-bond H2′-C2′ and H3′-C3′ couplings which improved the precision of the structures obtained recently for a 42 nucleotide RNA.
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Bax, A., Kontaxis, G. and Tjandra, N. (2001) Meth. Enzymol., 339, 127–174.
Brutscher, B., Boisbouvier, J., Pardi, A., Marion, D. and Simorre, J.P. (1998) J. Am. Chem. Soc., 120, 11845–11851.
de Alba, E. and Tjandra, N. (2002) Prog. NMR Spectrosc., 40(2), 175–197.
Geen, H. and Freeman, R. (1991) J. Magn. Reson., 93, 93–141.
Goddard, T. D. and Kneller, D. G. (2001) SPARKY 3, University of California, San Francisco.
Hansen, M.R., Muller, L. and Pardi, A. (1998) Nat. Struct. Biol., 5, 1065–1074.
Hoogstraten, C.G. and Pardi, A. (1998) J. Magn. Reson., 133, 236–240.
Marion, D., Ikura, M., Tschudin, R. and Bax, A. (1989) J. Magn. Reson., 85, 393–399.
Moore, P. B. (1995). Acc. Chem. Res., 28, 251–256.
Ottiger, M., Delaglio, F. and Bax, A. (1998) J. Magn. Reson., 131, 373–378.
Pardi, A. (1995) Meth. Enzymol., 261, 350–380.
Pardi, A. and Nikonowicz, E.P. (1992) J. Am. Chem. Soc., 114, 9202–9203.
Prestegard, J.H., Al-Hashimi, H.M. and Tolman, J.R. (2000) Q. Rev. Biophys., 33, 371–424.
Sibille, N., Pardi, A., Simorre, J.P. and Blackledge, M. (2001) J. Am. Chem. Soc., 123, 12135–12146.
Tian, F., Al-Hashimi, H.M., Craighead, J.L. and Prestegard, J.H. (2001) J. Am. Chem. Soc., 123, 485–492.
Tjandra, N. and Bax, A. (1997) J. Magn. Reson., 124, 512–515.
Tjandra, N., Tate, S., Ono, A., Kainosho, M. and Bax, A. (2000) J. Am. Chem. Soc., 122, 6190–6200.
Varani, G., Aboul-ela, F. and Allain, F.H.T. (1996) Prog. NMR Spectrosc., 29, 51–127.
Vermeulen, A., Zhou, H.J. and Pardi, A. (2000) J. Am. Chem. Soc, 122, 9638–9647.
Warren, J.J. and Moore, P.B. (2001) J. Biomol. NMR, 20,311–323.
Yan, J., Copora, T., Pradhan, P. and Bushweller, J.H. (2002) J. Biomol. NMR, 22, 9–20.
Zidek, L., Wu, H., Feigon, J. and Sklenar, V. (2001) J. Biomol. NMR, 21, 153–160.
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Vallurupalli, P., Moore, P.B. Measurement of H2′-C2′ and H3′-C3′ dipolar couplings in RNA molecules. J Biomol NMR 24, 63–66 (2002). https://doi.org/10.1023/A:1020620016891
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DOI: https://doi.org/10.1023/A:1020620016891