Abstract
Recently, there has been very rapid progress in understanding the transport of mineral nutrients across plant membranes. Genes for a number of primary ion pumps, cotransporters and ion channels have been cloned and the characteristics of their function are being investigated. While these advances have yet to produce cultivars that are better able to cope with nutrient deficient soils, they provide powerful tools to address some important gaps in our knowledge, particularly the regulation of transporter genes. While the current focus is on nutrient influx into roots, other processes are also significant in determining nutrient acquisition. This is illustrated by research on the relationship between the high affinity phosphate absorption mechanism and phosphate transport to the shoot, and with data on relating to the importance of phosphate efflux relative to influx. Molecular biology and plant physiology are providing information and technology that will be valuable in improving the suitability of crops for nutrient deficient environments. However, there is an urgent need for new ways to integrate this information so that the significance of individual processes to the performance of the whole system can be understood.
Similar content being viewed by others
References
Bieleski R L and Läuchli A 1992 Phosphate uptake, efflux and deficiency in the water fern, Azolla. Plant Cell Environ. 15, 665-673.
Cammack J N and Schwartz E A 1996 Channel behavior in a ?-aminobutyrate transporter. Proc. Natl. Acad. Sci. USA 93, 723-727.
Clark G T, Dunlop J and Phung H T 2000 Phosphate absorption by Arabidopsis thaliana: Interactions between phosphorus status and inhibition by arsenate. Aust. J. Plant Physiol. 27, (in press).
Chrispeels M J, Crawford N M and Schroeder J I 1999 Proteins for transport of water and mineral nutrients across the membranes of plant cells. Plant Cell 11, 661-675.
Clarkson D T and Grignon C 1991 The phosphate transport system and its regulation in roots. In Phosphorus Nutrition of Grain Legumes in the Semi-arid Tropics. Eds. C Johansen, KK Lee and KL Sarawat. pp. 49-62. ICRISAT, Pancheru, A.P., India.
Cornish-Bowden A 1999 Metabolic control analysis in biotechnology and medicine. Nature Biotech. 17, 641-643.
Crawford N M and Glass A D M 1998 Molecular and physiological aspects of nitrate uptake in plants. Trends Plant Sci. 3, 389-395.
Dong B, Rengel Z and Delhaize E 1998 Uptake and translocation of phosphate by pho2 mutant and wild-type seedlings of Arabidopsis thaliana. Planta 205, 251-256.
Dreyer I, Horeau C, Lemaillet G, Zimmerman S, Bush D R, Rodriguez-Navarro A, Schachtman D P, Spalding E P, Sentenac H and Gaber R 1999 Identification and characterisation of plant transporters using heterologous expression systems. J. Exp. Bot. 50, 1073-1087.
Dunlop J 1999 The physiology of phosphorus nutrition in plants-can we assemble the components into a practical model? In Plant Nutrition: Molecular Biology and Genetics. Eds. G Gissel-Nielsen and A Jensen. pp. 371-377. Kluwer Academic Publishers, Dordrecht, The Netherlands.
Dunlop J and Phung H T 1999 Efflux and influx as factors determining the relative abilities of ryegrass and white clover to compete for phosphate. In Plant Nutrition: Molecular Biology and Genetics. Eds. G Gissel-Nielsen and A Jensen. pp. 105-110. Kluwer Academic Publishers, Dordrecht, The Netherlands.
Epstein E 1966 Dual pattern of ion absorption by plant cells and by plants. Nature 212, 1324-1327.
Fairman W A, Vandenberg R J, Arriza J L, Kavanaugh M P and Amara S G 1995 An excitatory amino-acid transporter with the properties of a ligand-gated chloride channel. Nature 375, 599-603.
Fox T C and Guerinot M L 1998 Molecular biology of cation transport in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 49, 669-696.
Gassmann W, Rubio F and Schroeder J I 1996 Alkali cation selectivity of the wheat root high affinity potassium transporter HKT1. Plant J. 10, 869-882.
Hedrich R and Schroeder J I 1989 The physiology of ion channels and electrogenic pumps in higher plants. Ann. Rev. Plant Physiol. 40, 539-569.
Jackson P, Robertson R, Cooper M and Hammer G et al. 1996 The role of physiological understanding in plant breeding: from a breeding perspective. Field Crops Res. 49, 11-37.
Lappartient A G, Vidmar J J, Leustek T, Glass A D M and Touraine B 1999 Inter-organ signalling in plants: Regulation of ATP sulfurylase and sulfate transporter genes expression in roots mediated by phloem-translocated compound. Plant J. 18, 89-95.
Lauter R-R, Ninneman O, Bucher M, Riesmeier J W and Frommer W B 1996 Preferential expression of an ammonium transporter and two putative nitrate transporters in root hairs of tomato. Proc. Natl. Acad. Sci. 93, 8138-8144.
Lucas W J 1997 Application of microinjection techniques in plants. In Plant Nutrition for Sustainable Food Production and Environment. Developments in Plant and Soil Sciences, Volume 78. Eds. T Ando, K Fujita, T Mae, H Matsumoto, S Mori and J Sekiya. pp. 25-39. Kluwer Academic Publishers, Dordrecht, The Netherlands.
Macklon A E S, Lumsdon D G, Sim A and McHardy W J 1996 Phosphate fluxes, compartmentation and vacuolar speciation in root cortex cells of intact Agrostis capillaris seedlings: Effects of non-toxic levels of aluminium. J. Exp. Bot. 47, 793-803.
McPharlin I R and Bieleski R L 1989 Pi efflux and influx by P-adequate and P-deficient Spirodela and Lemna. Aust. J. Plant Physiol. 16, 391-399.
Maranville J W 1993 Adaptation of plants to soil stresses. Proceedings of a workshop at University of Nebraska, Lincoln, Nebraska. INTSORMIL Publication No. 94-2.
Rubio F, Gassmann W and Schroeder J I 1995 Sodium driven potassium uptake by the plant potassium transporter HKT1 and mutations conferring salt tolerance. Science 270, 1660-1663.
Scachtman D and Liu W 1999 Molecular pieces to the puzzle of the interaction between potassium and sodium uptake in plants. Trends Plant Sci. 4, 281-287.
Schachtman D P, Schroeder J I, Lucas W J, Anderson J A and Gaber R F 1992 Expression of an inward-rectifying potassium channel by the Arabidopsis KAT1 cDNA. Science 258, 1654-1658.
Sentenac H, Bonneaud N, Minet M, Lacroute F and Salmon J-M 1992 Cloning and expression in yeast of a plant potassium ion transport system. Science 256, 663-665.
Smith F W, Ealing P M, Hawkesford M J and Clarkson D T 1995a Plant members of a family of sulfate transporters reveal functional subtypes. Proc. Natl. Acad. Sci. USA 92, 9373-9377.
Smith F W, Ealing P M, Dong B and Delhaize E 1997 The cloning of two Arabidopsis genes belonging to a phosphate transporter family. Plant J. 11, 83-92.
Somerville C and Somerville S 1999 Plant functional genomics. Science 285, 380-383.
Sze H, Li X and Palmgren M J 1999 Energization of plant cell membranes by H+-pumping ATPases: Regulation and biosynthesis. Plant Cell 11, 677-689.
Taber R 1991 Knowledge processing with fuzzy cognitive maps. Exp. Syst. Appl. 2, 83-87.
Tanner W and Caspari T 1996 Membrane transport carriers Annu. Rev. Plant Physiol. Plant Mol. Biol. 47, 595-626.
Trewavas A 1986 Understanding the control of plant development and the role of growth substances. Aust. J. Plant Physiol. 13, 447-457.
Wallace D H and Yan W 1998 Plant breeding and whole-system crop physiology. CAB International, Wallingford, Oxon, UK. 631 p.
Wang R-L, Stec A, Hey J, Lukens L and Doebley J 1997 The limits of selection during maize domestication. Nature 398, 236-239.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Dunlop, J., Phung, T. Transporter genes to enhance nutrient uptake: Opportunities and challenges. Plant and Soil 245, 115–122 (2002). https://doi.org/10.1023/A:1020614111720
Issue Date:
DOI: https://doi.org/10.1023/A:1020614111720