Abstract
The activity of yeast alcohol dehydrogenase is markedly enhanced by Eu3+ ions. At pH 7.0 two binding constants for Eu3+, 1.0 × 10−2 and 2.0 × 10−3 μM, were obtained using a Scatchard plot. The presence of Zn2+ ions restricts the Eu3+-induced increase in the activity of yeast alcohol dehydrogenase. Studies on the tryptophan fluorescence of the enzyme in the absence and presence of Eu3+ or Zn2+ ions showed that Eu3+ affects tertiary or quaternary structures, which is consistent with its activation of the enzyme. The presence of Zn2+ reverses the conformational changes caused by Eu3+. Comparison of the effects of Eu3+ with Zn2+ for apo-yeast alcohol dehydrogenase indicates that their binding sites on the protein are different.
Similar content being viewed by others
REFERENCES
Beaucamp, K., Bergmeyer, H. U., and Beutler, H. O. (1974). In Methoden der Enzymatischen Analyse, 3rd ed. (Bergmeyer, H. U., ed.), Verlag Chemie, Weinheim, Germany, p. 580.
Bille, V., and Remacle, J. (1986). Eur. J. Biochem. 160, 343–348.
Buenzli, J. C. G. (1987). J. Phys. Coll. C 7, 607–610.
Coleman, P. L., and Weiner, H. (1973). Biochem. 12, 3466–3472.
Ganzhorn, A. J., and Plapp, B. V. (1988). J. Biol. Chem. 263, 5446–5454.
Hayes, J. E., Jr., and Valick, S. F. (1954). J. Biol. Chem. 207, 225–244.
Hunston, D. L. (1975). Anal. Biochem. 63, 99–106.
Jornvall, H., et al. (1978). J. Biol. Chem. 253, 8414–8419.
Kinman, J. P., and Welsh, K. (1976). Biochem. Biophsys. Res. Commun. 70, 874–884.
Klakamp Scott, L., and Horrocks, W. D., Jr. (1990). Biopolymers 30, 33–43.
Magonet, E., Hayen, P., Delforge, D., et al. (1992). Biochem. J. 287, 361–365.
McNemar, C. W., and Horrocks, W. D., Jr. (1990). Biochim. Biophys. Acta 1040, 229–236.
Veillon, C., and Sytkowski, A. J. (1975). Biochem. Biophsys. Res. Commun. 67, 1494–1500.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zhang, YX., Duan, CL. & Zhou, HM. Interaction of Eu3+ with Yeast Alcohol Dehydrogenase. J Protein Chem 18, 97–101 (1999). https://doi.org/10.1023/A:1020607818690
Published:
Issue Date:
DOI: https://doi.org/10.1023/A:1020607818690